Reproductive changes in male rats treated perinatally with an aromatase inhibitor

Gerardin, Daniela Cristina Ceccatto; Pereira, Olga

doi:10.1016/s0091-3057(01)00667-0

Cited by 45 publications

(19 citation statements)

References 19 publications

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“…Indeed, the proliferation of rat gonocytes is induced by 17 -oestradiol (Li et al 1997). Moreover, male rats treated perinatally with an aromatase inhibitor showed a decrease in the number of spermatozoa in the testis (Gerardin et al 2002). Hypogonadal mice, in which germ cell development never progresses beyond the pachytene stage, developed elongated spermatids after treatment with oestradiol (Ebling et al 2000).…”

Section: Discussionmentioning

confidence: 99%

Human immature germ cells and ejaculated spermatozoa contain aromatase and oestrogen receptors

Lambard¹,

Galeraud-Denis²,

Saunders³

et al. 2004

Journal of Molecular Endocrinology

122

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It is now well established that oestrogens play a part in germ cell function. These hormones are synthesised by the cytochrome P450 aromatase (P450 arom) and act via two kinds of receptor (ER and ER ). Although the presence of aromatase and oestrogen receptors in mammalian testis is now well documented, the localisation of these proteins in human germ cells is not yet clear. The primary purpose of the current study was to look for the expression of aromatase and oestrogen receptors in human germ cells. Human immature germ cells were collected from semen samples with an excess of rounds cells (>20%) and purified spermatozoa were obtained after sedimentation on a discontinuous PureSperm gradient. Expression of aromatase and oestrogen receptors was determined by RT-PCR with specific primers, and by Western blot using monoclonal antibodies. RT-PCR products for aromatase, ER and ER were amplified from total RNA isolated from human germ cells and spermatozoa. We identified an ER isoform variant that lacks exon 4 in human germ cells and visualised P450 arom as a single band of 49 kDa in germ cells, as we have already reported for human ejaculated spermatozoa. By Western blot, we identified two proteins for ER at ∼ 50 and ∼ 60 kDa, which could correspond to the long and short forms of ER formed from the use of alternative start sites. In human ejaculated spermatozoa, ER protein was not detected, even though we could amplify mRNA. Using Western blot analysis and a monoclonal antibody specific for ER , we detected two proteins in human immature germ cells: one of the expected size (66 kDa) and a second one of 46 kDa. In mature spermatozoa, only the 46 kDa band was observed and we speculate it may be related to the ER isoform lacking exon I. In conclusion, we have identified P450 arom and ER proteins (full-length and variant) in human germ cells. Further studies are now required to elucidate the mechanism of action of oestrogens on human male germ cells, in terms of both genomic and 'non-genomic' pathways.

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Section: Discussionmentioning

confidence: 99%

Human immature germ cells and ejaculated spermatozoa contain aromatase and oestrogen receptors

Lambard¹,

Galeraud-Denis²,

Saunders³

et al. 2004

Journal of Molecular Endocrinology

122

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“…There was a decrease in the number of spermatozoa found in the testes and in the daily sperm production. Only fifty percent of these males were capable of presenting male sexual behavior, while twenty five percent of the males did not present male sexual behavior, showing female sexual behavior when castrated and pretreated with estrogen (Gerardin et al, 2002). These results, plus data from Dohler (1991), demonstrated that the differentiation of the male rat hypothalamus is not exclusively estrogen dependent and that, during differentiation of the brain, estrogen is supportive of the primary actions of androgens.…”

Section: Long-termentioning

confidence: 66%

“…Conducts and/or exposure to some drugs in the perinatal period that apparently do not endanger the neonate may lead to later side effects (Carlos et al, 1996;Arena & Pereira, 2002;Gerardin & Pereira, 2002;Pereira et al, 2003 a,b,c ). Thus, their impact upon health and ecosystems is debated.…”

Section: Introduction Introduction Introduction Introduction Introducmentioning

confidence: 99%

<b>Endocrine Disruptors and Hypothalamic Sexual Differentiation<b/>

Pereira

2006

Annu Rev Biomed Sci

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Abstract Abstract AbstractThe so-called "endocrine disruptors" have been described as compounds which interfere with the estrogen action in their receptors and may exert a crucial role in the development of the reproductive tract and in the brain sexual differentiation. Thus, conducts and/or exposure to these drugs in the perinatal period that apparently do not endanger the neonate may cause side effects. During embrionary development, the gonads, through discharge of a small quantity of reproductive hormones, will guarantee the phenotype of male or female at birth, as well as actuate in specific areas sexual differentiation of the central nervous system. Several experimental models have shown an interference of drugs acting as endocrine disruptors in hypothalamic sexual differentiation. Thus, reproductive function is impaired by exposure to estrogen in the perinatal life of rats and the mechanisms involved in this effect are distinct for males and females. Perinatal exposure to drugs which may be considered endocrine disrupters may induce an incomplete masculinization and defeminization of the central nervous system. Alterations in these processes, if present, generally are perceived only at puberty or adult reproductive life. These later alterations may include anomalies in the process of fertility or in sexual behavior.

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“…Após a detecção da fê-mea em cio, esta foi colocada na gaiola de comportamen--to junto com uma rata isca por um período de 15 minutos. Os parâmetros observados foram: 1) latência para primeira monta e 2) número total de lordoses (GERAR-DIN; PEREIRA, 2002;GERARDIN et al, 2005GERARDIN et al, , 2006.…”

Section: Avaliação Do Comportamento Homossexual De Ratas Fêmeasunclassified

Efeito da administração do citrato de clomifeno durante o período perinatal no comportamento sexual, peso dos órgãos e concentração hormonal de ratos Wistar machos e fêmeas

Oliani

Dias

Naves

et al. 2015

Braz. J. Vet. Res. Anim. Sci.

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ResumoForam investigados os efeitos da exposição perinatal ao citrato de clomifeno no comportamento sexual, peso dos órgãos e concentração hormonal de ratos machos e fêmeas. Os animais receberam quatro doses de 2 mg/mL de citrato de clomifeno, no período perinatal (21 dias de gestação -DG21), nos dias 1 (DN1), 2 (DN2) e 3 (DN3) após o nascimento dos filhotes. O tratamento causou desenvolvimento de ovário policístico em 70% das fêmeas, masculinização do comportamento sexual das fêmeas e alteração do comportamento sexual dos machos evidenciado pela redução no número de ejaculações. Em relação aos níveis hormonais, observou-se diminuição de FSH na prole masculina. Concluiu-se que o citrato de clomifeno interfere na capacidade reprodutiva de ratos machos e fêmeas, e na orientação sexual de fêmeas, quando administrado perinatalmente.Palavras-chave: Desruptores endócrinos. Antiestrogênio. Comportamento homossexual. Ejaculação. AbstractThe effects of prenatal exposure to clomiphene citrate in sexual behavior, organ weight and hormone concentrations of male and female rats was evaluated. The animals received four doses of clomiphene citrate 2 mg/mL each during the prenatal period (21 days of gestation -DG21) on days 1 (DN1), 2 (DN2) and 3 (DN3) after the birth of the puppies. The treatment led to the development of polycystic ovaries in 70% of the females, masculinization of female sexual behavior and changes in sexual behavior of males evidenced by the reduction in the number of ejaculations. In regards to hormone levels, a decrease in the FSH levels in male offspring was observed. It was concluded that clomiphene citrate interferes with the reproductive capacity of male and female rats and female sexual orientation when prenatally administered. Keywords

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Reproductive changes in male rats treated perinatally with an aromatase inhibitor

Cited by 45 publications

References 19 publications

Human immature germ cells and ejaculated spermatozoa contain aromatase and oestrogen receptors

Human immature germ cells and ejaculated spermatozoa contain aromatase and oestrogen receptors

<b>Endocrine Disruptors and Hypothalamic Sexual Differentiation<b/>

Efeito da administração do citrato de clomifeno durante o período perinatal no comportamento sexual, peso dos órgãos e concentração hormonal de ratos Wistar machos e fêmeas

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