Reproductive biology of middle Mississippi River shovelnose sturgeon: insights from seasonal and age variation in plasma sex steroid and calcium concentrations

Whitledge, Gregory W.; Kelly, Anita M.

doi:10.1111/j.1439-0426.2009.01334.x

Cited by 15 publications

(14 citation statements)

References 34 publications

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“…Variations in several blood parameters have been reported to be characteristic of pre‐spawning and spawning periods, and significantly different levels were found among sex and maturity stages. A physiological increase in plasma Mg ++ concentration, as observed in this study, was reported for example as a function of vitellogenesis in A. transmontanus , A. persicus and Scaphirhynchus platarynchus (Linares‐Casenave et al., 2003; Asadi et al., 2006b; Stahl et al., 2009). Similarly, an increase in plasma albumin concentration may be linked to increased steroid hormone transportation (Shahsavani et al., 2010).…”

Section: Discussionsupporting

confidence: 82%

Assessment of blood chemistry reference values for cultured sturgeon hybrids (Acipenser naccarii female × Acipenser baerii male)

Marco

Finoia

Petochi

et al. 2011

Journal of Applied Ichthyology

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Summary Blood chemistry profiles of cultured sturgeon hybrids Acipenser naccarii female × Acipenser baerii male were assessed in order to acquire basic data for evaluating fish health status on‐farm. Sixty healthy fish (11.0 ± 3.2 Kg weight; 117.8 ± 8.6 cm total length) were randomly captured from 600 m3 concrete tanks and sampled from April to June. Blood samples were analyzed for the following 22 biochemical parameters using standard clinical methods: cortisol, glucose, lactate, non esterified fatty acids, triglycerides, cholesterol, albumin, creatinine, urea, total bilirubin, total protein, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, creatine phosphokinase, lactate dehydrogenase, calcium, phosphorus, magnesium, sodium, potassium and chloride. Biochemical values were found to be consistent with data from the parental species A. naccarii. Cortisol, cholesterol, albumin, urea, total bilirubin, CK, LDH and magnesium were significantly affected by sampling time and the differences are probably related to sexual maturation of the fish. Blood chemistry analyses afforded an accurate evaluation of sturgeons’ health status, also providing information on their reproductive conditions. Evidence resulting from cortisol and lactate values indicated that pre‐sampling operations, including capture and confinement of these large‐sized fish, must be carefully managed in order to minimize handling stress. This study provides knowledge on blood chemistry of hybrid sturgeon A. naccarii × A. baerii and the results corroborate the validity of the test panel performed in evaluating the physiological functions of sturgeons. Reference values for 22 biochemical parameters were identified for the purpose of evaluating health and welfare in cultured sturgeons.

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Section: Discussionsupporting

confidence: 82%

Assessment of blood chemistry reference values for cultured sturgeon hybrids (Acipenser naccarii female × Acipenser baerii male)

Marco

Finoia

Petochi

et al. 2011

Journal of Applied Ichthyology

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“…Coincident with these changes in [Ca 2+ ] were declines in circulating [K + ] and [Na + ], which were resolved by day 7. Plasma [Ca 2+ ] is known to be associated with vitellogenesis and can be used as a predictor of reproductive status in the Acipenserid species examined to date (Van Eenennaam et al., ; Linares‐Casenave et al., ; Stahl et al., ). Thus, the observed increase in [Ca 2+ ] is likely related to reproductive status, and furthermore the concentration measured is in accord with plasma [Ca 2+ ] during spawning in other Acipenser species (McEnroe and Cech, ; Williot et al., ).…”

Section: Discussionmentioning

confidence: 99%

Induced spawning of wild-caught adult lake sturgeon: assessment of hormonal and stress responses, gamete quality, and survival

Genz

McDougall²,

Burnett

et al. 2014

J. Appl. Ichthyol.

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SummaryThis research assessed the endocrine and physiological effects of Ovaprim TM and synthetic homologous sturgeon gonadotrophic releasing hormone (GnRH) on spawning male and female adult lake sturgeon, Acipenser fulvescens (Rafinesque, 1817). Wild-caught adults were administered Ovaprim, GnRH, or Ringers solution (control), and serial blood samples were collected for up to 27 days following capture and hormone administration. Circulating cortisol levels appeared to increase following administration of both hormonal treatments. However, circulating cortisol was higher and plasma pH lower at capture than at any other time for all treatments, indicating that capture and transport stress were the largest disrupting factors, more so than the effects of induced spawning. Plasma concentrations of estradiol and testosterone in both sexes were similar to previously reported values. In all treatments the circulating estradiol and testosterone never significantly exceeded the values at capture prior to endocrine manipulation of the reproductive axis. Similarly, testosterone in males and estradiol in females in the muscle tissue were consistently at the same or lower concentrations than at capture. The apparent survival of all of the study animals following return to their native river indicated that the stress effects from capture and induced spawning were not severe. In concert, our results suggest that hormonal injections can be used to aid in gamete collection from wild A. fulvescens, without negative effects on longterm broodstock survival. Hatching success, larval survival to the juvenile stage, egg diameter, and concentrations of protein, triglyceride, and glucose in the egg, sperm, and ovarian fluid (but not seminal plasma), were the same or greater in GnRH-treated fish than gametes from the Ovaprim-treated group, suggesting that utilization of GnRH may be preferable to other common methods of spawning induction.

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“…The ratio of ovarian follicles achieving ovulatory competence could therefore be used effectively to estimate the timing of hormonal treatment by evaluating follicular maturity. Some species such as white sturgeon and shovelnose sturgeon ( Scaphirhynchus platorynchus ) may also spawn in autumn (Conte et al., ; Stahl et al., ). In fact, peaks in plasma estradiol‐17β, Ca 2+ and testosterone in shovelnose sturgeon were observed not only in April but also in autumn (Stahl et al., ).…”

Section: Discussionmentioning

confidence: 99%

“…Some species such as white sturgeon and shovelnose sturgeon ( Scaphirhynchus platorynchus ) may also spawn in autumn (Conte et al., ; Stahl et al., ). In fact, peaks in plasma estradiol‐17β, Ca 2+ and testosterone in shovelnose sturgeon were observed not only in April but also in autumn (Stahl et al., ). The ratio of follicles that acquired ovulatory competence first increased in November or January; thus, eggs may be collected during those months.…”

Section: Discussionmentioning

confidence: 99%

Seasonal changes in oocyte maturational competence, ovulatory competence and gonadotropin sensitivity of ovarian follicles in hybrid sturgeon, bester (Huso huso × Acipenser ruthenus )

et al. 2014

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Seasonal changes in oocyte maturational competence, ovulatory competence and gonadotropin sensitivity of ovarian follicles in hybrid sturgeon, bester (Huso huso 3 Acipenser ruthenus) SummaryOocyte maturation and ovulation in sturgeon species are induced by injection of hormones such as the luteinizing hormone-releasing hormone (LHRH). Appropriate timing for the LHRH injection is determined by monitoring the maturational stage of the ovarian follicles. In the present study, ovarian follicles from a group of female bester sturgeon (Huso huso 9 Acipenser ruthenus) were sampled continuously from September to May and incubated in 100% L-15 medium (pH 8.2) containing 17a-hydroxyprogesterone or salmon pituitary extract (SPE) for 48 h at 15°C. After incubation, ratios of oocyte maturational competence (OMC; ability of oocytes to undergo final oocyte maturation in response to maturation-inducing steroid, MIS), ovulatory competence (OC; ability of ovarian follicles to ovulate under MIS stimulation), and GTH sensitivity (ability of ovarian follicles to respond to GTH) were examined. Almost all oocytes maintained OMC throughout the sampling period. However, OC was acquired in circa December and May. The ratio of follicles that acquired ovulatory competence showed wide variation among individuals. Most females first acquired a higher degree of ovulatory competence in May, although some only did so around December. SPE-induced oocyte maturation was relatively high throughout the entire experiment, and the ovulation rate showed a tendency similar to that of the ovulatory competence. LHRH-a injections in May successfully induced ovulation in all females. Higher hatching rates resulted in females whose ovarian follicles showed an increasing trend of ovulatory competence and GTH sensitivity for ovulation in May. These results indicate that ovulatory competence and GTH sensitivity can be used as indicators to determine the timing of LHRH injections for sturgeon. U.S.

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Reproductive biology of middle Mississippi River shovelnose sturgeon: insights from seasonal and age variation in plasma sex steroid and calcium concentrations

Abstract: Additional research is needed to elucidate fall spawning by shovelnose sturgeon in the MMR.3

Cited by 15 publications

References 34 publications

Assessment of blood chemistry reference values for cultured sturgeon hybrids (Acipenser naccarii female × Acipenser baerii male)

Assessment of blood chemistry reference values for cultured sturgeon hybrids (Acipenser naccarii female × Acipenser baerii male)

Induced spawning of wild-caught adult lake sturgeon: assessment of hormonal and stress responses, gamete quality, and survival

Seasonal changes in oocyte maturational competence, ovulatory competence and gonadotropin sensitivity of ovarian follicles in hybrid sturgeon, bester (Huso huso × Acipenser ruthenus )

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