Reproductive biology of Actaea kashmiriana, an endemic medicinal plant species from the Himalaya: Implications for conservation and sustainable utilisation

Incidence and frequency of desynapsis in Eremurus persicus (Jaub. & Spach) Boiss.(Asphodelaceae) – A native and important medicinal plant species of Western Himalaya

“…In present study meiotic abnormalities were found in the target species, similarly meiotic abnormalities were also found in E, himalaicus growing in Indian Himalayan region (Kumari et al, 2016). The abnormalities are attributed to pervading environmental conditions in the Himalaya (Wani et al 2023). The phenomenon of desynapsis was also found in the PMCs of presently studied plant species, both extrinsic conditions such as temperature and fertilizer quality (Dhesi et al, 1975;Rao, 1975) and intrinsic conditions like gene action, loss of chromosome pair, apomixis and structural or numeral changes of chromosomes (Praaken, 1943) are responsible for the desynaptic behavior of plants.…”

“…In first method, mature anthers were squashed in 1% acetocarime and kept for 10 minutes and then slides were scanned under microscope, well stained and plump pollen grains were considered viable while as lightly stained and shriveled pollen grains were considered as non-viable. In second method, the ready-to-dehisce mature anthers were placed in 1% tetrazolium chloride for one hour and squashed to check for viability (Rashid et al 2023) and in third method pollen grains were mounted in Fluorescein diacetate (FDA) solution and incubated for 3-5 min. The pollens with fluorescent and non-fluorescent cytoplasm were treated as viable and nonviable, respectively (Rashid et al 2023).…”

“…In second method, the ready-to-dehisce mature anthers were placed in 1% tetrazolium chloride for one hour and squashed to check for viability (Rashid et al 2023) and in third method pollen grains were mounted in Fluorescein diacetate (FDA) solution and incubated for 3-5 min. The pollens with fluorescent and non-fluorescent cytoplasm were treated as viable and nonviable, respectively (Rashid et al 2023). In all the three methods, percentage of pollen viability was determined by following formula:…”

“…The meiotic abnormalities observed in E. persicus may be attributed to high fruit and seed abortion rates. Most conservation biologists believe that before setting up a conservation program for plant species it is better to know about its intrinsic reproductive constraints (Friedman & Ryerson, 2009;Wani et al,. 2023).…”

Global research progress on reproductive behavior and ethnobotany of the Saussurea genus: Literature review-based-bibliometric analysis

“…Information on reproductive biology of any plant species is crucial to their successful conservation and sustainable utilization (Rashid et al 2023). In the absence of data on species is considered to be threatened when mortality surpasses births for a significant period of time because of limitations on their reproduction.…”