Reproductive Biology

Aiken, D. E.; Waddy, S. L.

doi:10.1016/b978-0-12-177401-1.50011-9

Cited by 106 publications

(77 citation statements)

References 90 publications

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“…The reproductive stage of each animal was determined by the method of Aiken & Waddy (1980), in which a combination of weight, colour and developmental stage of the ovary is used as the criterion.…”

Section: Animalsmentioning

confidence: 99%

“…The eyestalks were then ablated for extirpation of the X-organ and the sinus gland. Finally the ovaries were dissected and their exact developmental stage was determined using weight, colour and ovary factor (Aiken & Waddy 1980) as parameters.…”

Section: Experimental Set Upmentioning

confidence: 99%

“…While all CHH isoforms have a hyperglycaemic effect, CHH-B can also stimulate oocyte growth (Tensen et al 1989) and CHH-A may display molt-inhibiting activity (Chang et al 1991). Moreover, the effects of eyestalk ablation and implantation of thoracic/abdominal ganglia indicated the presence of a vitellogenic-stimulating hormone (Otsu 1963, Gomez & Nayar 1965, Aiken & Waddy 1980). These results, in combination with recent studies showing that CHH-A and -B mRNAs are present in parts of the nervous system other than the optic ganglia, indicate that CHH may have an additional role in the control of reproduction and molting (De Kleijn et al 1995).…”

Section: Introductionmentioning

confidence: 99%

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Expression of the crustacean hyperglycaemic hormones and the gonad-inhibiting hormone during the reproductive cycle of the female American lobster Homarus americanus

Kleijn

Janssen²,

Waddy³

et al. 1998

Journal of Endocrinology

103

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Crustacean reproduction is regulated by a complex chain of hormonal interactions in which the crustacean hyperglycaemic hormones A and B (CHH-A and CHH-B) and the gonad-inhibiting hormone (GIH) play a primary role. These neurohormones are produced in the same neuroendocrine cells of the X-organ sinus gland complex, situated in the eyestalks of the American lobster, Homarus americanus. In order to obtain more information on the synthesis, storage, release and function of these three neuropeptides during the reproductive cycle, we studied the levels of their mRNAs in the X-organ, their peptide storage in the sinus gland and their concentration in the haemolymph at different stages of the female reproductive cycle. A high CHH-A mRNA level was found only in the previtellogenic stage, while elevated mRNA levels were determined for CHH-B in the mature as well as the previtellogenic stage. High CHH storage levels in the sinus gland were found during previtellogenesis. The total amount of CHH (CHH-A plus -B) in the haemolymph was significantly higher during maturation. A low level of GIH mRNA in the X-organ and a low amount of the GIH I isoform in the sinus gland were found only in the immature stage. In contrast, GIH haemolymph levels were high during the immature and previtellogenic stages. We conclude that CHH-A and -B are involved in triggering the onset of vitellogenesis and that CHH-B in particular is responsible for stimulating oocyte maturation before spawning, while GIH prevents the start of vitellogenesis in the ovary. Moreover, our results show that the balance between the haemolymph levels of the CHHs and GIH may tune the synchronization of reproduction and molting during the biannual reproductive cycle of the American lobster.

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Section: Animalsmentioning

confidence: 99%

Section: Experimental Set Upmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Expression of the crustacean hyperglycaemic hormones and the gonad-inhibiting hormone during the reproductive cycle of the female American lobster Homarus americanus

Kleijn

Janssen²,

Waddy³

et al. 1998

Journal of Endocrinology

103

View full text Add to dashboard Cite

show abstract

“…Males become sexually functional at a slightly smaller size. Mating (between a newly molted female and a hard shelled male) and oviposition are independent events separated by as little as one month to as much as two years (Aiken and Waddy, 1980). Fertilization· occurs at oviposition.…”

Section: Background Information Life History and Development Of The Amentioning

confidence: 99%

“…The larval and postlarval lobsters were molt staged by the method of Sasaki (1984) which was based on the method described by Aiken (1973Aiken ( , 1980 for molt staging adult lobsters.…”

Section: Experimental Animals: Gravid Femalesmentioning

confidence: 99%