Reproductive ability of second generation ornamental (koi) carp ( Cyprinus carpio L.) × goldfish ( Carassius auratus L.) hybrids and characteristics of their offspring

Abstract

The purpose of this study was to investigate the reproductive ability of second generation (F 2 ) koi (Cyprinus carpio L.) 9 goldfish (Carassius auratus L.) hybrids. Only

How low can they go: A comparison between dog (Canis familiaris) and environmental DNA detection of invasive koi carp (Cyprinus rubrofuscus)

“…False positive errors (false alarms) occurred on both non-target sample types but were more common with goldfish samples. This indication bias is not surprising given that carp and goldfish are sufficiently related to be able to produce hybrid offspring and thus likely have similar scent profiles (Chistiakov and Voronova, 2009;Banks et al, 2010;Hernández-Roldán et al, 2014;Warner et al, 2018). False positive errors could have also been due to cross-contamination, or the high ratio of target and non-target samples (9 targets to 8 non-targets; 53% chance of a target sample) (Lazarowski et al, 2020;Edwards et al, 2022).…”

Growth and survival of diploid and triploid bata, Labeo bata (Hamilton, 1822)

“…Although an equivalent amount of FI was observed (no significant difference; p > 0.05) between diploid and triploid groups, triploids exhibited significantly higher performance (p < 0.05) for all other growth parameters (Table 3). These results Differences in growth performance between triploids and diploids (superior growth performance of triploids in most cases) are hypothesised to be due to: differences in cell volume and heterozygosity, presence of an extra chromosome set and the induced sterility in triploids due to an imbalanced distribution of chromosomes during meiosis (Carter et al, 1994;Malison et al, 1993;O'Keefe & Benfey, 1997;Rahi et al, 2017;Warner et al, 2018;Withler et al, 1998;Yamazaki & Goodier, 1993). Triploids usually contain 33% additional genetic material in each nucleus (Rahi & Shah, 2012a;Tave, 1992;Vargas et al, 2015).…”

“…Triploidy (three chromosome sets) is a ploidy manipulation technique, successfully applied in improving aquaculture production and fisheries management goals over the last few decades (Rahi & Shah, 2012a;Shah et al, 1999;Warner et al, 2018). Triploidy induces sterile populations of fish (Gheyas et al, 2001), and triploids are produced directly by the retention of the second polar body during the second meiotic division.…”

“…Triploidy is often induced by heat shock, cold shock, pressure shock and chemical shock, but it may also be generated indirectly by the crossing of tetraploid and diploid individuals (Rahi & Shah, 2012b;Tave, 1992). Theoretically, triploid fishes are expected to grow faster than the normal diploids, as triploid cells contain 33% more genetic material due to the presence of an extra chromosome set (Rahi & Shah, 2012a;Warner et al, 2018). Moreover, sterility is another important factor for the faster growth of triploids over their diploid counterparts, as triploids can easily divert their reproductive energy towards somatic growth (Wasow et al, 2004).…”

Reproductive ability of triploid ornamental (koi) carp ( Cyprinus carpio L.) × goldfish ( Carassius auratus L.) hybrids and characteristics of their offspring

“…Flow cytometry was used in this study for determination of fish ploidy instead of karyotyping since the accuracy and convenience of this method for analysis of both euploid and aneuploid cyprinid fish were shown earlier (Delomas, Gomelsky, Anil, Schneider, & Warner, 2017;Gomelsky, Delomas, & Warner, 2016;Gomelsky, Schneider, Anil, & Delomas, 2015;Gomelsky, Warner, Delomas, Novelo, & Kramer, 2019;Warner, Gomelsky, Delomas, Kramer, & Novelo, 2018). Ploidy of broodstock and juveniles obtained from triploid females was determined by flow cytometric analysis of nuclear DNA content in erythrocyte nuclei using an Accuri C6 flow cytometer (BD Biosciences) according to a technique described by Delomas et al (2017) and Warner et al (2018). Blood samples were taken from brood fish and some juveniles using a 3.0-ml Vacutainer tube containing lithium heparin (Becton, Dickenson and Co.).…”