Reproductive Ability After Uterine Artery Embolization in a Sheep Model: Observation Over 2 Seasons

Yamagami, Takuji; Yoshimatsu, Rika; Matsumoto, Tomohiro; Anzai, H.; Yoshizawa, Masahiro; Fukui, Yutaka

doi:10.1177/1933719110369010

Cited by 2 publications

(2 citation statements)

References 18 publications

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“…aries ) not only provides meat, milk, wool, cashmere and fur but is also an important model organism for cardiology research [1,2], reproductive medicine [3], respiratory medicine [4,5] and many other fields. Understanding the genetic principles of meat traits would be helpful for promoting sheep growth and for basic biology and human medicine.…”

Section: Introductionmentioning

confidence: 99%

Characterization and Comparative Analyses of Muscle Transcriptomes in Dorper and Small-Tailed Han Sheep Using RNA-Seq Technique

Zhang

Wang

et al. 2013

PLoS ONE

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The sheep is an important domestic animal and model for many types of medically relevant research. An investigation of gene expression in ovine muscle would significantly advance our understanding of muscle growth. RNA-seq is a recently developed analytical approach for transcriptome profiling via high-throughput sequencing. Although RNA-seq has been recently applied to a wide variety of organisms, few RNA-seq studies have been conducted in livestock, particularly in sheep. In this study, two cDNA libraries were constructed from the biceps brachii of one Small-tailed Han sheep (SH) and one Dorper sheep (DP). The Illumina high-throughput sequencing technique and bioinformatics were used to determine transcript abundances and characteristics. For the SH and DP libraries, we obtained a total of 50,264,608 and 52,794,216 high quality reads, respectively. Approximately two-thirds of the reads could be mapped to the sheep genome. In addition, 40,481 and 38,851 potential coding single nucleotide polymorphisms (cSNPs) were observed, respectively, of which a total of 59,139 cSNP coordinates were different between the two samples. Up to 5,116 and 5,265 respective reference genes had undergone 13,827 and 15,684 alternative splicing events. A total of 6,989 reference genes were extended at the 5’, 3’ or both ends, and 123,678 novel transcript units were found. A total of 1,300 significantly differentially expressed genes were identified between the two libraries. These results suggest that there are many differences in the muscle transcriptomes between these two animals. This study addresses a preliminary analysis and offers a foundation for future genomic research in the sheep.

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Section: Introductionmentioning

confidence: 99%

Characterization and Comparative Analyses of Muscle Transcriptomes in Dorper and Small-Tailed Han Sheep Using RNA-Seq Technique

Zhang

Wang

et al. 2013

PLoS ONE

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“…The sheep is an established model organism for medically relevant research in cardiology [1,2], reproductive medicine [3], respiratory medicine [4,5] and many other fields. The sheep is particularly important in the field of orthopedics, because the dimensions of ovine long bones allow the use of implants designed for application in humans, and the bone mineral composition as well as the metabolic and remodeling rates are similar to those in humans [6-10].…”

Section: Introductionmentioning

confidence: 99%

Composite transcriptome assembly of RNA-seq data in a sheep model for delayed bone healing

et al. 2011

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BackgroundThe sheep is an important model organism for many types of medically relevant research, but molecular genetic experiments in the sheep have been limited by the lack of knowledge about ovine gene sequences.ResultsPrior to our study, mRNA sequences for only 1,556 partial or complete ovine genes were publicly available. Therefore, we developed a composite de novo transcriptome assembly method for next-generation sequence data to combine known ovine mRNA and EST sequences, mRNA sequences from mouse and cow, and sequences assembled de novo from short read RNA-Seq data into a composite reference transcriptome, and identified transcripts from over 12 thousand previously undescribed ovine genes. Gene expression analysis based on these data revealed substantially different expression profiles in standard versus delayed bone healing in an ovine tibial osteotomy model. Hundreds of transcripts were differentially expressed between standard and delayed healing and between the time points of the standard and delayed healing groups. We used the sheep sequences to design quantitative RT-PCR assays with which we validated the differential expression of 26 genes that had been identified by RNA-seq analysis. A number of clusters of characteristic expression profiles could be identified, some of which showed striking differences between the standard and delayed healing groups. Gene Ontology (GO) analysis showed that the differentially expressed genes were enriched in terms including extracellular matrix, cartilage development, contractile fiber, and chemokine activity.ConclusionsOur results provide a first atlas of gene expression profiles and differentially expressed genes in standard and delayed bone healing in a large-animal model and provide a number of clues as to the shifts in gene expression that underlie delayed bone healing. In the course of our study, we identified transcripts of 13,987 ovine genes, including 12,431 genes for which no sequence information was previously available. This information will provide a basis for future molecular research involving the sheep as a model organism.

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Reproductive Ability After Uterine Artery Embolization in a Sheep Model: Observation Over 2 Seasons

Abstract: The mid-term influence of UAE on reproductive ability in sheep was minimal.

Cited by 2 publications

References 18 publications

Characterization and Comparative Analyses of Muscle Transcriptomes in Dorper and Small-Tailed Han Sheep Using RNA-Seq Technique

Characterization and Comparative Analyses of Muscle Transcriptomes in Dorper and Small-Tailed Han Sheep Using RNA-Seq Technique

Composite transcriptome assembly of RNA-seq data in a sheep model for delayed bone healing

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