Reproduction of bovine keratoconjunctivitis with a purified haemolytic and cytotoxic fraction of Moraxella bovis

Beard, M.K. McG.; Moore, Lindsey

doi:10.1016/0378-1135(94)90074-4

Cited by 54 publications

(5 citation statements)

References 22 publications

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“…The MbxA secreted by M. bovis is implicated in the pathogenesis of infectious bovine keratoconjunctivitis (Brown et al , 1998), where it promotes corneal epithelial cell damage (Rogers et al , 1987; Beard & Moore, 1994;). The mbxCABD genes form a classical rtx operon that is absent from nonhemolytic strains (Angelos et al , 2003) and is part of a mobile genetic element designated as a pathogenicity island of M. bovis (Hess & Angelos, 2006).…”

Section: Classes Of Rtx Proteinsmentioning

confidence: 99%

RTX proteins: a highly diverse family secreted by a common mechanism

et al. 2010

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Repeats-in-toxin (RTX) exoproteins of Gram-negative bacteria form a steadily growing family of proteins with diverse biological functions. Their common feature is the unique mode of export across the bacterial envelope via the type I secretion system and the characteristic, typically nonapeptide, glycine- and aspartate-rich repeats binding Ca2+ ions. In this review, we summarize the current state of knowledge on the organization of rtx loci and on the biological and biochemical activities of therein encoded proteins. Applying several types of bioinformatic screens on the steadily growing set of sequenced bacterial genomes, over 1000 RTX family members were detected, with the biological functions of most of them remaining to be characterized. Activities of the so far characterized RTX family members are then discussed and classified according to functional categories, ranging from the historically first characterized pore-forming RTX leukotoxins, through the large multifunctional enzymatic toxins, bacteriocins, nodulation proteins, surface layer proteins, up to secreted hydrolytic enzymes exhibiting metalloprotease or lipase activities of industrial interest.

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Section: Classes Of Rtx Proteinsmentioning

confidence: 99%

RTX proteins: a highly diverse family secreted by a common mechanism

et al. 2010

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“…Pili enable M. bovis to adhere to the corneal surface (Annuar and Wilcox 1985; Moore and Rutter 1989; Ruehl et al 1993a). The M. bovis cytotoxin lyses bovine neutrophils, erythrocytes, lymphocytes, and corneal epithelial cells leading to corneal ulceration (Beard and Moore 1994; Gray et al 1995; Hoien-Dalen et al 1990; Kagonyera et al 1989a; Kagonyera et al 1989b). The M. bovis cytotoxin (MbxA) is an RTX (repeats in the structural toxin) toxin (Angelos et al 2001) that resides within an RTX operon on a pathogenicity island in M. bovis (Angelos et al 2003; Hess and Angelos 2006).…”

Section: Introductionmentioning

confidence: 99%

Recombinant Moraxella bovoculi cytotoxin-ISCOM matrix adjuvanted vaccine to prevent naturally occurring infectious bovine keratoconjunctivitis

et al. 2010

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A randomized, blinded, controlled field trial was conducted during summer 2006 in a northern California, USA, herd of beef cattle to evaluate the efficacy of a recombinant Moraxella bovoculi cytotoxin subunit vaccine to prevent naturally occurring infectious bovine keratoconjunctivitis (IBK; pinkeye). A convenience sample comprised of 127 steers were administered a subcutaneous dose of either adjuvant alone (ISCOM matrices; control group) or recombinant M. bovoculi cytotoxin carboxy terminus adjuvanted with ISCOM matrices (MbvA group) and were boostered 21 days later. The steers were examined once weekly for 15 weeks for evidence of IBK. No significant difference in the cumulative proportion of corneal ulcerations was detected between groups. Compared to the control calves, the MbvA vaccinates had significantly higher increases in serum neutralizing titers to M. bovoculi hemolysin between week 0 and week 6. The prevalence of M. bovis isolations was higher from ulcerated eyes of calves vaccinated with MbvA as compared to control calves. Vaccination of calves against the carboxy terminus of M. bovoculi RTX toxin resulted in significant increases in serum hemolysin neutralizing titers and may modulate organism type cultured from ulcerated eyes of calves in herds where both M. bovis and M. bovoculi exist. Use of M. bovoculi antigens alone in vaccines to prevent IBK may not be beneficial in herds where IBK is associated with both M. bovoculi and M. bovis.

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“…The M. bovis cytotoxin (hemolysin), MbxA [18] , is an RTX toxin that is also necessary for pathogenesis [19] , [20] , [21] . MbxA is conserved among geographically diverse isolates [22] and IBK-affected cattle develop antihemolysin antibodies that cross-neutralize hemolysin from diverse strains of M. bovis [23] .…”

Section: Introductionmentioning

confidence: 99%

Randomized controlled field trial to assess the efficacy of an intranasal Moraxella bovis cytotoxin vaccine against naturally occurring infectious bovine keratoconjunctivitis

Angelos,

Agulto,

Mandzyuk

et al. 2023

Vaccine: X

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Reproduction of bovine keratoconjunctivitis with a purified haemolytic and cytotoxic fraction of Moraxella bovis

Cited by 54 publications

References 22 publications

RTX proteins: a highly diverse family secreted by a common mechanism

RTX proteins: a highly diverse family secreted by a common mechanism

Recombinant Moraxella bovoculi cytotoxin-ISCOM matrix adjuvanted vaccine to prevent naturally occurring infectious bovine keratoconjunctivitis

Randomized controlled field trial to assess the efficacy of an intranasal Moraxella bovis cytotoxin vaccine against naturally occurring infectious bovine keratoconjunctivitis

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