Reproduction effectiveness of carp (Cyprinus carpio L.) from the Hungarian W breeding line after stimulating ovulation with spawning inducing agents of natural (CPH, hCG, PMSG) and/or synthetic origin (Ovopel, Dagin, Ovaprim, mGnRH-a)

Brzuska, E

doi:10.1016/j.aquaculture.2020.736023

Cited by 17 publications

(7 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These doses refer to the published amount for the ovulation of Cyprinids. 30 Fourteen to 16 h after hormone injection, broodstock ovulation and spermiation occurred. Eggs were collected individually into a plastic vessel and spermatozoa were collected with sterile syringes.…”

Section: Methodsmentioning

confidence: 99%

Broodstock development, induced spawning and larval rearing of the bilih, Mystacoleucus padangensis (Bleeker, 1852), a vulnerable species, and its potential as a new aquaculture candidate

Syandri,

Azrita,

Thamrin

et al. 2023

F1000Res

View full text Add to dashboard Cite

Background: Mystacoleucus padangensis living in Lake Singkarak, Indonesia, has high potential market demand but is threatened by overfishing and has not been successfully cultured. This study describes the first broodstock development, induced breeding, and larval rearing of M. padangensis. Methods: A total of 1,000 female and 1,000 male broodfish were collected from the wild and reared in two concrete ponds (128 m2) at the Centre for Biodiversity Conservation, P.T. Semen Padang, Indonesia. The broodfish were fed commercial feed to satiation at 09:00 and 17:00 h. The females (average weight 7.56 ± 0.85 g) and males (4.86 ± 1.20 g) were selected at a ratio of 1:4 (female:male), and gonad maturation was induced with a single dose of GnRH analogue (Ovaprim) of 0.1 ml/fish. At 16 h after hormone injection, eggs were collected individually into a plastic vessel. Spermatozoa were collected with sterile syringes. Eggs were fertilized using the "dry" method, and 0.5 ml samples (equal to 100 eggs) were taken. The eggs were incubated in a plastic strainer with a water volume of 1.57 litres and placed in a tarpaulin pond with a volume of 150.72 litres. Results: The overall hatching rate was 78.93 ± 4.13%. The newly hatched larvae were 3900.81 µm long, with a yolk sac of 82881.480 µm2. The mouth opened at 72 days post hatching (DPH) with a gape measuring approximately 61.880 µm. The protocol of larval feeding started with artificial feed, followed by Artemia nauplii up to 30 DPH. Weaning of larvae started at 4 DPH. Larvae started metamorphosis by 15 DPH and ended by 22 DPH when the larvae reached 7430.27 µm. Larval rearing resulted in an average survival rate of 28.4 ± 3.04%. Conclusions: Its successful spawning induction and high larval hatching and survival rates make M. padangensis an excellent aquaculture candidate.

show abstract

Section: Methodsmentioning

confidence: 99%

Broodstock development, induced spawning and larval rearing of the bilih, Mystacoleucus padangensis (Bleeker, 1852), a vulnerable species, and its potential as a new aquaculture candidate

Syandri,

Azrita,

Thamrin

et al. 2023

F1000Res

View full text Add to dashboard Cite

show abstract

“…These doses refer to the published amount for the ovulation of Cyprinids. 25 Fourteen to 16 h after hormone injection, broodstock ovulation and spermiation occurred. Eggs were collected individually into a plastic vessel and spermatozoa were collected with sterile syringes.…”

Section: Assessment Of Oocyte Maturational Development and Artificial...mentioning

confidence: 99%

Broodstock development, induced spawning and larval rearing of the bilih, Mystacoleucus padangensis (Bleeker, 1852), a vulnerable species, and its potential as a new aquaculture candidate

Syandri

Azrita

Thamrin³

et al. 2023

F1000Res

View full text Add to dashboard Cite

Background: Mystacoleucus padangensis living in Lake Singkarak, Indonesia, has high potential market demand but is threatened by overfishing and has not been successfully cultured. This study describes the first broodstock development, induced breeding, and larval rearing of M. padangensis. Methods: A total of 1,000 female and 1,000 male broodfish were collected from the wild and reared in two concrete ponds (128 m2) at the Centre for Biodiversity Conservation, P.T. Semen Padang, Indonesia. The broodfish were fed commercial feed to satiation at 09:00 and 17:00 h. The females (average weight 7.56 ± 0.85 g) and males (4.86 ± 1.20 g) were selected at a ratio of 1:4 (female:male), and gonad maturation was induced with a single dose of GnRH analogue (Ovaprim) of 0.1 ml/fish. At 16 h after hormone injection, eggs were collected individually into a plastic vessel. Spermatozoa were collected with sterile syringes. Eggs were fertilized using the "dry" method, and 0.5 ml samples (equal to 100 eggs) were taken. The eggs were incubated in a plastic strainer with a water volume of 1.57 litres and placed in a tarpaulin pond with a volume of 150.72 litres. Results: The overall hatching rate was 78.93 ± 4.13%. The newly hatched larvae were 3900.81 µm long, with a yolk sac of 82881.480 µm2. The mouth opened at 72 DPH with a gape measuring approximately 61.880 µm. The protocol of larval feeding started with artificial feed, followed by Artemia nauplii up to 30 DPH. Weaning of larvae started at 4 DPH. Larvae started metamorphosis by 15 DPH and ended by 22 DPH when the larvae reached 7430.27 µm. Larval rearing resulted in an average survival rate of 28.4 ± 3.04%. Conclusions: Its successful spawning induction and high larval hatching and survival rates make M. padangensis an excellent aquaculture candidate.

show abstract

“…During the maturation stage of the ovary, E2 causes the synthesis of 17α,20β-DHP in the steroidogenic pathway, which induces the final maturation of the oocyte [3,4]. An oocyte maturates due to 17α,20β-DHP through the stimulation of maturation-promoting factor formation in oocytes [5]. A similar important role is played by genes in the regulation of ovarian development.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the Ovarian Development and Associated Factors during the Breeding Migration of Coilia nasus in the Yangtze River

Wei,

Hua,

Yang

et al. 2024

Fishes

View full text Add to dashboard Cite

Coilia nasus is a typical anadromous migratory fish found in the lower reaches of the Yangtze River. Every year, C. nasus clusters offshore and swims upstream along the Yangtze River into the tributaries and lakes in the middle and lower reaches of the Yangtze River to breed. In this study, female C. nasus were collected as study subjects from the Chongming section of Shanghai, the Taizhou section of Jiangsu, and the Anqing section of Anhui. Their ovaries were used to examine tissue sections and investigate gene expression, including the follicle-stimulating hormone receptor (fshr), the luteinizing hormone receptor (lhr), kisspeptin-1 (kiss1), and forkhead box l2 (foxl2), which are related to reproductive development, while the serum levels of estrogen (including estradiol, E2) and progestins (including 17α,20β-dihydroxy-4-pregenen-3-one, 17α,20β-DHP) were also analyzed. Our results showed that, first, the growth period of the oocytes was small in stage II of ovarian development, in which both E2 and 17α,20β-DHP levels and gene expression were low. Then, in stage III, the growth period of the oocytes became large, and the yolk granules and oil droplets began to appear. Simultaneously, E2 and the expression of kiss1 and foxl2 were significantly elevated. Finally, stage IV was the period of a large amount of accumulation of nutrients in the oocytes, and 17α,20β-DHP levels and the expression of fshr and lhr were significantly elevated. These results enrich the theoretical study of ovarian development in the natural population of C. nasus, supplementing the biological basis of C. nasus reproduction and scientifically supporting the study of C. nasus population ecology and resource conservation.

show abstract

Reproduction effectiveness of carp (Cyprinus carpio L.) from the Hungarian W breeding line after stimulating ovulation with spawning inducing agents of natural (CPH, hCG, PMSG) and/or synthetic origin (Ovopel, Dagin, Ovaprim, mGnRH-a)

Cited by 17 publications

References 37 publications

Broodstock development, induced spawning and larval rearing of the bilih, Mystacoleucus padangensis (Bleeker, 1852), a vulnerable species, and its potential as a new aquaculture candidate

Broodstock development, induced spawning and larval rearing of the bilih, Mystacoleucus padangensis (Bleeker, 1852), a vulnerable species, and its potential as a new aquaculture candidate

Broodstock development, induced spawning and larval rearing of the bilih, Mystacoleucus padangensis (Bleeker, 1852), a vulnerable species, and its potential as a new aquaculture candidate

Characterization of the Ovarian Development and Associated Factors during the Breeding Migration of Coilia nasus in the Yangtze River

Contact Info

Product

Resources

About