Reproduction disturbances of Brachionus calyciflorus (rotifer) for the screening of environmental endocrine disrupters

Radix, P.; Severin, Gabriele F.; Schramm, K.‐W.; Kettrup, A.

doi:10.1016/s0045-6535(01)00335-6

Cited by 64 publications

(42 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Nevertheless, because only the asexual portion of the life cycle is commonly investigated in toxicity studies, the true vulnerability of zooplankton to toxicants is often underestimated (Preston and Snell 2001;Navis et al 2015). Several studies assessed the effects of pesticides, heavy metals, and endocrine-disrupting chemicals on sexual reproductive parameters of rotifers (Snell and Carmona 1995;Preston et al 2000;Yoshinaga et al 2000;Preston and Snell 2001;Radix et al 2002;Marcial et al 2005;Ríos-Arana et al 2007) and cladocerans (Tatarazako and Oda 2007;Navis et al 2013Navis et al , 2015. Their results showed that, depending on the species and contaminants tested, reproductive parameters such as mixis ratio (i.e., proportion of sexual females relative to all females, sexual and asexual) and fertilization, and diapause parameters such as resting egg production and resting egg hatchability are among the most sensitive endpoints to assess toxicity in zooplankton species.…”

Section: Introductionmentioning

confidence: 99%

Diapause as escape strategy to exposure to toxicants: response of Brachionus calyciforus to arsenic

Aránguiz-Acuña

Serra

2016

Ecotoxicology

View full text Add to dashboard Cite

Invertebrate organisms commonly respond to environmental fluctuation by entering diapause. Production of diapause in monogonont rotifers involves a previous switch from asexual to partial sexual reproduction. Although zooplankton have been used in ecotoxicological assays, often their true vulnerability to toxicants is underestimated by not incorporating the sexual phase. We experimentally analyzed traits involved in sexual reproduction and diapause in the cyclically parthenogenetic freshwater rotifer, Brachionus calyciflorus, exposed to arsenic, a metalloid naturally found in high concentrations in desert zones, focusing on the effectiveness of diapause as an escape response in the face of an adverse condition. Addition of sublethal concentrations of arsenic modified the pattern of diapause observed in the rotifer: investment in diapause with arsenic addition peaked earlier and higher than in non-toxicant conditions, which suggests that sexual investment could be enhanced in highly stressed environmental conditions by increased responsiveness to stimulation. Nevertheless, eggs produced in large amount with arsenic, were mostly low quality, and healthy-looking eggs had lower hatching success, therefore it is unclear whether this pattern is optimum in an environment with arsenic, or if rather arsenic presence in water bodies disturbs the optimal allocation of offspring entering diapause. We observed high accumulation of arsenic in organisms exposed to constant concentration after several generations, which suggests that arsenic may be accumulated transgenerationally. The sexual phase in rotifers may be more sensitive to environmental conditions than the asexual one, therefore diapause attributes should be considered in ecotoxicological assessment because of its ecological and evolutionary implications on lakes biodiversity.

show abstract

Section: Introductionmentioning

confidence: 99%

Diapause as escape strategy to exposure to toxicants: response of Brachionus calyciforus to arsenic

Aránguiz-Acuña

Serra

2016

Ecotoxicology

View full text Add to dashboard Cite

show abstract

“…Discussion Halbach (1984) was among the first person to use population growth as a tool for evaluating the effects of toxicants. Several workers later have used this approach for many species of rotifers such as B. plicatilis, B. calyciflorus and B. patulus to quantify the effects of pesticides (i.e., DDT, lindane, thiophanate-methyl, glyphosate, diazinon, fenitrothion, methoprene, isoprothiolane, cypermethrin, deltamethin, dicofol, endosulfan, atrazine and carbaryl) Sarma, 1986, 1990;Janssen et al, 1994;Xi and Feng, 2004;Chu et al, 2005;Marcial et al, 2005;Xi et al, 2007;Huang et al, 2011;Lu et al, 2012) and other pollutants (i.e., growth hormone, human chorionic gonadotropin, 17b-estradiol, triiodothyronine, 20-hydroxyecdysone, 5-hydroxytryptamine, c-aminobutyric acid, juvenile hormone, ethinylestradiol, nonylphenol, testosterone, phthalate acid esters and melamine) (Gallardo et al, 1997;Radix et al, 2002;Zhao et al, 2007;Wen et al, 2011).…”

Section: Resultsmentioning

confidence: 99%

“…However, the detailed reasons for their different effects need further research in the future. Radix et al (2002) found that nonylphenol at concentrations from 129.94 to 599.05 mg.L x1 significantly increased the OF/NOF in B. calyciflorus population, but the reverse was true for ethinylestradiol at concentrations higher than 201.55 mg.L x1 . Meanwhile, testosterone did not significantly influence it, which was similar to butyl benzyl phthalate, but both 5000 mg.L x1 di-n-butyl phthalate and 5-5000 mg.L x1 di (2-ethylhexyl) phthalate significantly increased the OF/NOF .…”

Section: Resultsmentioning

confidence: 99%

“…All the rotifers were continually cultured for another 24 h to count the number of RE (if any). Meanwhile, the population growth rate (r, r= (ln N t xln N 0 )/t, where N 0 and N t are 2nd and final rotifer population density, respectively, and t = 3), the ratio of ovigerous females to non-ovigerous females (OF/NOF), the ratio of mictic females to amictic females (MF/AF), the mictic rate (MR), and the fertilization rate (FR) were calculated according to Radix et al (2002).…”

Section: Methodsmentioning

confidence: 99%

“…However, only a portion of the rotifer life cycle or other endpoints are investigated in these studies, the true vulnerability of rotifer life cycle to toxicants is often underestimated (Preston and Snell, 2001). Hence, several studies assessed the effects of pesticides, heavy metals and endocrine disrupting chemicals on the entire life cycle of rotifers (Snell and Carmona, 1995;Preston et al, 2000;Preston and Snell, 2001;Radix et al, 2002;Xi et al, 2007). These results showed that sexual reproduction and resting egg production are among the most sensitive endpoints.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Responses in the population growth and reproduction of freshwater rotiferBrachionus calyciflorusto four organochlorine pesticides

Huang

Zha

et al. 2013

Ann. Limnol. - Int. J. Lim.

View full text Add to dashboard Cite

-In China, although the production and use of organochlorine pesticides (OCPs) have been banned for decades, relatively high levels of OCP residues have still been found in some water bodies, and can result in adverse acute and chronic effects on zooplankton including rotifers, which have caused public concern for many years. Responses in the population growth and reproduction of freshwater rotifer Brachionus calyciflorus to four OCPs including aldrin, dieldrin, b-hexachlorocyclohexane (b-HCH) and chlordecone were studied by 3-day population growth and 4-day resting eggs (RE) production tests. In comparison with control, aldrin at 10 mg.L x1 , b-HCH at 1000 mg.L x1 and chlordecone at 0.05 mg.L x1 significantly increased the population growth rate (r); but aldrin at 100 mg.L x1 , dieldrin at 0.001 and 0.1 mg.L x1 , b-HCH at 0.1-100 mg.L x1 and chlordecone at 50 mg.L x1 markedly decreased it. Aldrin at concentrations higher than 1 mg.L x1 , dieldrin at 0.01 and 1000 mg.L x1 , b-HCH at concentrations 0.1 and higher than 1 mg.L x1 , and chlordecone at concentrations 0.005 and higher than 0.5 mg.L x1 significantly decreased the ratio of ovigerous females to non-ovigerous females (OF/NOF), but the reverse was true for aldrin at 0.1 mg.L x1 and b-HCH at 0.001 mg.L x1 . Dieldrin at 0.001, 0.01 and 1000 mg.L x1 significantly decreased the ratio of mictic females to amictic females (MF/AF), but b-HCH at 1 and 10 mg.L x1 highly significantly increased it. Dieldrin at 1000 mg.L x1 and b-HCH at concentrations higher than 10 mg.L x1 markedly decreased the fertilization rate (FR). Both aldrin and chlordecone have no significant effect on the MF/AF and FR of rotifers. Aldrin at concentrations higher than 1 mg.L x1 , dieldrin at lower than 0.1 and higher than 10 mg.L x1 , b-HCH at 1000 mg.L x1 and chlordecone at 0.005, 0.05 and 50.0 mg.L x1 significantly decreased the mictic rate (MR) of rotifers, but the reverse was true for b-HCH at 1 mg.L x1 . Aldrin at 10 mg.L x1 , dieldrin at 0.001, 0.1 and 1000 mg.L x1 , b-HCH at concentrations higher than 1 mg.L x1 and chlordecone at concentrations higher than 0.005 mg.L x1 markedly decreased RE production of rotifers, but b-HCH at 0.01 mg.L x1 significantly increased it. A clear dose-response relationship existed between the RE and the concentration of dieldrin, b-HCH and chlordecone, and the OF/NOF and the aldrin concentration. The RE and OF/NOF in rotifer population might be suitable endpoints for monitoring the low concentration of three OCPs (dieldrin, b-HCH and chlordecone) and aldrin, respectively.

show abstract

Effects of Anthropogenic Estrogens Nonylphenol and 17?-Ethinylestradiol in Aquatic Model Ecosystems

Hense

Severin

Pfister

et al. 2005

Acta hydrochim. hydrobiol.

View full text Add to dashboard Cite

Microcosm tests were conducted to investigate the effects of the estrogenic substances nonylphenol (NP) and 17α‐ethinylestradiol (EE) on aquatic ecosystems. Maximum concentrations of 9 to 120 μg L—1 NP resp. 49 to 724 ng L—1 EE were induced by controlled release. The controlled release method allows the establishment of a continuous concentration course. The microcosms proved to run robustly with abiotic conditions close to natural. They developed biocenosis with similar characteristics as in natural ecosystems and, considering their given level of complexity, they can be used to describe possible risks for the environment. Both tested chemicals unveiled the potential to affect the plankton communities in the tested concentration range. NP exposure caused a reduction of Cladocera and Copepoda abundances and disturbed the phytoplankton structure. A NOECcommunity of 30 μg L—1 was calculated. In the first EE study, a flood in the lake where the microcosm water was collected caused additional stress and thereby a high variability, both between the microcosms and in each microcosm over time. Probably therefore the only effect found was a reduction of Copepoda abundance. In a second EE study Cladocera and Copepoda abundances were reduced, from which the phytoplankton benefited. Although a final interpretation is difficult for results of microcosm tests, there are indications that the found effects of EE and perhaps also NP may be caused at least partially by endocrine disruptive activity.

show abstract

Reproduction disturbances of Brachionus calyciflorus (rotifer) for the screening of environmental endocrine disrupters

Cited by 64 publications

References 9 publications

Diapause as escape strategy to exposure to toxicants: response of Brachionus calyciforus to arsenic

Diapause as escape strategy to exposure to toxicants: response of Brachionus calyciforus to arsenic

Responses in the population growth and reproduction of freshwater rotiferBrachionus calyciflorusto four organochlorine pesticides

Effects of Anthropogenic Estrogens Nonylphenol and 17?-Ethinylestradiol in Aquatic Model Ecosystems

Contact Info

Product

Resources

About