Reproducibility of Thickness Measurements of Macular Inner Retinal Layers Using SD-OCT With or Without Correction of Ocular Rotation

Abstract

Citation: Hirasawa H, Araie M, Tomidokoro A, et al. Reproducibility of thickness measurements of macular inner retinal layers using SD-OCT with or without correction of ocular rotation. Invest Ophthalmol Vis Sci. 2013;54:256254: -257054: . DOI:10.1167 PURPOSE. To evaluate the intervisit reproducibility of spectral-domain optical coherence tomography (SD-OCT) measurement of the macular retinal nerve fiber layer thickness (mRNFLT); combined ganglion cell layer and inner plexiform layer (GCLþIPL) thickness; and ganglion cell complex (GCC) thicknesses (sum of mRNFLT and GCLþIPL thicknesses) compared with that of circumpapillary RNFLT (cpRNFLT) and the effect of ocular rotation on reproducibility.METHODS. SD-OCT imaging was performed twice on different days in one eye of 58 normal subjects and 73 glaucoma patients. The reproducibility was evaluated for the entire 4.8-mm 3 4.8-mm macular area and subareas (upper and lower halves, 2 3 2, 4 3 4, and 8 3 8 grids), and the 3608, upper, and lower halves mean cpRNFLT with and without correction of ocular rotation.RESULTS. The coefficients of variation (CVs) of GCLþIPL and GCC thickness measurements averaged below 1.0% for the entire and upper and lower half macular areas, and below 4.2% in the macular subareas in normal and glaucoma eyes, which were significantly smaller (P < 0.001) than those of mRNFLT measurements in the same areas of the same eyes. The CVs of mRNFLT measurements were significantly smaller than those of the cpRNFLT only in the lower half mean area in normal eyes. The reproducibility was minimally affected by correction of ocular rotation or presence of glaucoma.CONCLUSIONS. The reproducibility of the macular (GCLþIPL) and GCC thickness measurements was better than that of mRFNLT and cpRNFLT in normal and glaucoma eyes and minimally affected by correction of ocular rotation.

Reproducibility and Repeatability of Ganglion Cell-Inner Plexiform Layer Thickness Measurements in Healthy Subjects

“…Our intersession results were as excellent as in previous studies [2,9,10]. However, none of these studies had assessed interobserver intrasession reproducibility, which may be of some interest when comparing clinical trials data.…”

“…In a recent study [10] assessing the reproducibility of thickness measurements of macular inner retinal layers using SD-OCT with or without correction of ocular rotation, an SD-OCT device (3D OCT-1000, version 2.13; Topcon, Inc.) was used to evaluate the intervisit reproducibility of several parameters, including GC-IPL, using the CV, reproducibility coefficient and ICC. The authors reported better and excellent reproducibility of the macular GC-IPL and GCC thickness compared with that of the RNFL measurement in the macular and circumpapillary areas.…”

“…Recent studies [2,9,10] reported excellent intersession and intervisit reproducibility of macular GC-IPL measurement performed by a single operator both for healthy and glaucomatous eyes.…”

The Foveal Position Relative to the Optic Disc and the Retinal Nerve Fiber Layer Thickness Profile in Myopia

“…11 However, small amounts of changes in physiological ocular rotation exist among each test session, which may affect the intertest measurement reproducibility of peripapillary RNFL thicknesses. 27 Therefore, in this study, we compensated for the potential effect of the ocular rotation on the disc-foveal angle by using fundus photographs overlaid on spectral-domain (SD)-OCT images (Fig. 1).…”

Diagnostic ability of a new method for measuring haemoglobin levels in the optic nerve head in multiple sclerosis patients

“…Most authors consider that methods with a COV less than 10% have high reproducibility, while a COV less than 5% indicates very high reproducibility 13. To assess the reliability of the repeated measurements, the intraclass correlation coefficients for absolute agreement was calculated.…”