Reproducibility: changing the policies and culture of cell line authentication

Freedman, Leonard P.; Gibson, Mark C.; Ethier, Stephen P.; Soule, Howard R.; Neve, Richard M.; Reid, Yvonne

doi:10.1038/nmeth.3403

Cited by 120 publications

(102 citation statements)

References 40 publications

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“…An indispensable condition for fast implementation of new clinical approaches is that researchers worldwide use identical models to generate results that can validate each other's findings. Global Biological Resource Centres (BRCs) are equipped with authentication standards to ensure an optimal use of these models (Almeida et al, 2016;Barallon et al, 2010;Capes-Davis and Neve, 2016;Freedman et al, 2015). A recent survey revealed a reproducibility crisis in biomedical science, since more than 70% of researchers have failed to reproduce published experiments (Baker, 2016a), which in cancer research can be partly attributed to the use of different batches of FBS, as will be discussed below.…”

Section: Reliability Of Scientific Data Using In Vitro Models Role Omentioning

confidence: 99%

Fetal bovine serum (FBS): Past – present – future

Valk

Bieback

Buta³

et al. 2018

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Section: Reliability Of Scientific Data Using In Vitro Models Role Omentioning

confidence: 99%

Fetal bovine serum (FBS): Past – present – future

Valk

Bieback

Buta³

et al. 2018

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“…A key problem when using these methods is that quality control is too often lacking. A number of concerns have been increasingly discussed in recent years (Marx, 2014;Freedman et al, 2015). These have raised awareness of quality problems in cell culture experiments of which the most frequent with serious impact on the quality of research and products are cross-contamination and microbial infection.…”

Section: Introduction To Gccpmentioning

confidence: 99%

Good Cell Culture Practice for stem cells and stem-cell-derived models

Pamies

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Simeonov

et al. 2016

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SummaryThe first guidance on Good Cell Culture Practice (GCCP) dates back to 2005. This document expands this to include aspects of quality assurance for in vitro cell culture focusing on the increasingly diverse cell types and culture formats used in research, product development, testing and manufacture of biotechnology products and cell-based medicines. It provides a set of basic principles of best practice that can be used in training new personnel, reviewing and improving local procedures, and helping to assure standard practices and conditions for the comparison of data between laboratories and experimentation performed at different times. This includes recommendations for the documentation and reporting of culture conditions. It is intended as guidance to facilitate the generation of reliable data from cell culture systems, and is not intended to conflict with local or higher level legislation or regulatory requirements. It may not be possible to meet all recommendations in this guidance for practical, legal or other reasons. However, when it is necessary to divert from the principles of GCCP, the risk of decreasing the quality of work and the safety of laboratory staff should be addressed and any conclusions or alternative approaches justified. This workshop report is considered a first step toward a revised GCCP 2.0.

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“…Finally, our message to institutional hierarchy and other stakeholders such as funding agencies, reviewers, and editors of scientific journals is to continue to raise awareness of this problem and devise stricter guidelines and policies to change the current culture. 27,28 Corporation, Carpinteria, CA, USA) to determine epithelial cell content, a polyclonal antibody against the proliferation marker Ki-67 (F) (clone RB-1510-P1, Ab-4; Neomarkers, Fremont, CA, USA), a monoclonal Ab to the putative limbal epithelial SC marker K15 (G) (clone LHK15, Ab-1; Neomarkers), and appropriate isotype control Abs (G, inset). Some cells (>P23) were analyzed by RT-PCR for stem, corneal, and conjunctival marker mRNA expression (H) as previously described.…”

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confidence: 99%