Reproducibilidad de pruebas serológicas para el diagnóstico de infección por Trypanosoma cruzi en gestantes de zona endémica de Santander, Colombia

Castellanos, Yeny Zulay; Cucunubá, Zulma M.; Flórez, Astrid Carolina; Orozco-Vargas, Luís Carlos

doi:10.7705/biomedica.v34i2.1571

Cited by 6 publications

(5 citation statements)

References 24 publications

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“…On the other hand, in an analysis of the external quality evaluation program carried out during 15 years in Chile, it was reported that the sensitivity of the total ELISA methods was in the range of 96.9 % to 100 % with a specificity that oscillated between 76.4 % and 100 %. As for recombinant ELISA methods, the sensitivity was 100 %, with a specificity ranging from 94 % to 98.8 %, ranges in which our results are found 23 .…”

Section: Discussionsupporting

confidence: 62%

Diagnostic evaluation of two enzyme-linked immunosorbent assay tests for Trypanosoma cruzi infection in a Colombian population

Carrero

Monroy-Díaz

Tadger-Viloria³

et al. 2022

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Objective: To evaluate the diagnostic accuracy of two enzyme-linked immunosorbent assay tests to determine Trypanosoma cruzi infection in a Colombian population. Material and methods: Analytical study with case-control design of a diagnostic evaluation. Sensitivity, specificity and diagnostic efficacy of total CHAGATEK ELISA and recombinant CHAGATEK ELISA were determined. Positive predictive value, negative predictive value, and positive and negative likelihood ratios were calculated. The kappa coefficient was found to estimate the concordance between the index and reference tests. The discriminatory capacity of the tests was expressed by the area under the ROC curve. Results: Of the 184 samples included in the study, 99 were negative for T. cruzi and 85 were positive according to the reference tests used. Sensitivity for total and recombinant ELISA was 97.6 % and 98.8 %, respectively; while specificity was 97.0% and 94.9%. The level of concordance of the total and recombinant ELISA was almost perfect and the discriminatory ability of the two tests were good. Conclusions: It was demonstrated that the two tests can confirm and exclude the diagnosis of Chagas disease and have a good diagnostic performance.

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Section: Discussionsupporting

confidence: 62%

Diagnostic evaluation of two enzyme-linked immunosorbent assay tests for Trypanosoma cruzi infection in a Colombian population

Carrero

Monroy-Díaz

Tadger-Viloria³

et al. 2022

Infect

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“…Furthermore, a recent study in Colombia compared the intra-reproducibility of serological tests for identification of T. cruzi infection in pregnant women (ELISA, IFI and indirect hemagglutination-HAI in serum and ELISA on filter paper). This study concluded that the ELISA reproducibility in serum was 0.98, higher than the reproducibility of the other serological tests and well above the ELISA reproducibility on filter paper, which was 0.55 25 . the Stat-Pack test in population from 5 to 18 years of age in the department of Casanare, with a sensitivity of 85.4% and specificity of 98% when compared with conventional serological tests and 46.7% when compared to ELISA on filter paper, showing a lower sensitivity in relation to the studies 31 .…”

Section: Section 2 Diagnosis In Pregnant Womenmentioning

confidence: 60%

First Colombian consensus on congenital Chagas and clinical approach for women of child-bearing age diagnosed with Chagas

Cucunubá¹,

Valencia‐Hernández²,

Puerta

et al. 2017

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Cómo citar este artículo: Z.M. Cucunubá, et al. First Colombian consensus on recommendations for diagnosis and treatment of congenital Chagas and clinical approach for diagnosis in child-bearing age women diagnosed with Chagas. Infectio 2017; 21(4): [255][256][257][258][259][260][261][262][263][264][265][266] AbstractCongenital transmission of Chagas disease has not been extensively studied in Colombia, and there are no standardized processes in the health system regarding the specific diagnosis, treatment and follow-up of this disease. To generate recommendations on congenital Chagas disease and Chagas in women of childbearing age in Colombia, a consensus of experts was developed. An extensive literature search through the Medline database was carried out using the MeSH terms: «Chagas disease/congenital», «prevention and control», «diagnosis», «therapeutics» and «pregnancy». Appropriate abstracts were selected and the full texts were analyzed. The relevant information was synthesized, classified, and organized into tables and figures and was presented to a panel of experts, which was composed of 30 professionals from various fields. Based on the Delphi methodology, three rounds of consultation were conducted. The first and second rounds were based on electronic questionnaires that measured the level of consensus of each question among the participants. The third round was based on a face-to-face discussion focusing on those questions without consensus in the previous consultations. The evidence was adapted to national circumstances on a case-by-case basis, and the content the final document was approved. These recommendations are proposed for use in routine medical practice by health professionals in Colombia. Primer consenso colombiano sobre Chagas congénito y orientación clínica a mujeres en edad fértil con diagnóstico de Chagas ResumenLa transmisión congénita de la enfermedad de Chagas ha sido poco estudiada en Colombia y existen pocos procedimientos rutinarios en el sistema de salud para el manejo de esta enfermedad. Por ello se desarrolló un consenso de expertos dirigido a generar recomendaciones de diagnóstico y tratamiento de Chagas congénito y orientación a mujeres en edad fértil. Con ese propósito se realizó una búsqueda extensiva de la literatura, empleando una combinación de términos Mes (Chagas, Chagas congénito, prevención, control, diagnóstico, tratamiento y embarazo) para reflejar el estado del arte en cada tema de interés. Después de ello, se leyeron los resúmenes y aquellos seleccionados para análisis del texto completo. La literatura relevante se sintetizo, clasifico y organizo en tablas y se presentó al panel de expertos, el cual estaba constituido por 30 profesionales en diferentes áreas. Mediante la metodología Delphi se realizaron 2 rondas de cuestionarios virtuales y una reunión presencial en los cuales se evaluaron los niveles de acuerdo entre los participantes. Los puntos con falta de consenso durante las 2 rondas virtuales se expusieron durante las mesas de discusión en la ronda prese...

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“…In blood bank screening, the most commonly-used methods are those based on enzyme-linked immunosorbent assays (ELISA) and chemiluminescent microparticle immunoassays (CMIA), with more complex techniques, such as indirect immunofluorescence (IFI) and immunoblotting, generally being used for confirmatory purposes. Previously, high specificity and sensitivity levels (up to 95% of IFI standards) have been reported for ELISA and CMIA assays, as well as a high reproducibility of ELISA, IFI, and indirect hemagglutination (IHA) (29,30), but they also exhibit limitations that should be considered and analyzed through comparative studies with modern complementary techniques (29). For example, the false seropositive results reported in these tests have been explained by the crossreactivity with other hemoflagelate protozoa such as Leishmania spp.…”

Section: Discussionmentioning

confidence: 99%

Disagreement between PCR and serological diagnosis of Trypanosoma cruzi infection in blood donors from a Colombian endemic region

et al. 2021

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Introduction: Chagas’ disease is the leading cause of infectious myocarditis worldwide. This infection caused by Trypanosoma cruzi is usually life-long and asymptomatic; however, the third part of infected people can develop severe or even fatal cardiomyopathy. As the parasitemia in the chronic phase is both low-grade and intermittent, T. cruzi infection is principally detected by serology, although this method has sensitivity and specificity limitations.Objective: To determine the level of agreement between serologic and molecular tests in 658 voluntary blood donors from six provinces in the Colombian department of Santander.Materials and methods: We evaluated an array of diagnostic technologies by cross-section sampling performing a serological double diagnostic test for T. cruzi antibody detection (Chagas III ELISA™, BiosChile Group, and ARCHITECT Chagas CMIA™, Abbott), and DNA detection by polymerase chain reaction (PCR). We collected the demographic, clinical, and epidemiological information of participants. The sample size was calculated using Epidat™ and the statistical analysis was done with Stata 12.1™.Results: PCR was six times more sensitive in detecting T. cruzi infection than ELISA/CMIA with prevalence values of 1.8% (12/658) and 0.3% (2/658), respectively, and kappa=0.28 (95%CI: -0.03 - 0.59). In contrast, serology showed a sensitivity of 16.7% (95%CI: 2.09 - 48.4) and a specificity of 100% (95%CI: 99.4 - 100). All seropositive samples were found to be positive by PCR.Conclusions: The implementation of PCR as a complementary method for screening donors could reduce the probability of false negative and the consequent risk of transfusional-transmission of Chagas’ disease, especially in endemic regions.

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Reproducibilidad de pruebas serológicas para el diagnóstico de infección por Trypanosoma cruzi en gestantes de zona endémica de Santander, Colombia

Cited by 6 publications

References 24 publications

Diagnostic evaluation of two enzyme-linked immunosorbent assay tests for Trypanosoma cruzi infection in a Colombian population

Diagnostic evaluation of two enzyme-linked immunosorbent assay tests for Trypanosoma cruzi infection in a Colombian population

First Colombian consensus on congenital Chagas and clinical approach for women of child-bearing age diagnosed with Chagas

Disagreement between PCR and serological diagnosis of Trypanosoma cruzi infection in blood donors from a Colombian endemic region

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