1979
DOI: 10.1016/0005-2787(79)90130-8
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Repression of rRNA synthesis induced by disaggregation in Dictyostelium discoideum

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Cited by 14 publications
(10 citation statements)
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“…We have previously shown that disaggregation of Dictyostelium discoideum slugs induces repression of rRNA synthesis [18]. In the present study, it was found that the addition of 1 mM cAMP to disaggregated cells had no suppressive effect on the repression of rRNA synthesis, despite the fact that cAMP is known to suppress many disaggregation-induced metabolic changes in this organism.…”
Section: Discussioncontrasting
confidence: 51%
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“…We have previously shown that disaggregation of Dictyostelium discoideum slugs induces repression of rRNA synthesis [18]. In the present study, it was found that the addition of 1 mM cAMP to disaggregated cells had no suppressive effect on the repression of rRNA synthesis, despite the fact that cAMP is known to suppress many disaggregation-induced metabolic changes in this organism.…”
Section: Discussioncontrasting
confidence: 51%
“…discoideum strain NC-4 was used. Procedures for cell growth, slug disaggregation, and RNA extraction are described in [18]. Labeling of cells was conducted in a liquid culture containing 5 p, Ci/ml [5-3H]uracil (30.1 Ci/mmol NEN) in the presence or absence of 1 mM cAMP (Sigma).…”
Section: Methodsmentioning
confidence: 99%
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“…The cells were grown in a liquid medium that contained the following per liter: 10 g bacto-peptone (Difco, Detroit, U.S.A.), 10g glucose, 0.96g Na2HPO4-12H2O, and 1.45 g KH2PO4 (22). Escherichia coli strain B/r was the food source for the myxoamoebae.…”
Section: Methodsmentioning
confidence: 99%
“…Cells of the cellular slime mold, Dictyostelium discoideum strains NC4 and Ax2, and Dictyostelium mucoroides, were grown for 48 h at 22 qC with Escherichia coli B/r as the source of food for the myxamoebae in a suspension culture that contained bacto-peptone, glucose, Na 2 HPO 4 and KH 2 PO 4 (UCHIYAMA et al 1979). Cells were harvested by centrifugation and washed three times to free them from bacteria with BONNER's salt solution that contained NaCl, KCl and CaCl 2 (BONNER 1947).…”
Section: Preparation Of Cells and The Cell-free Crude Extractmentioning
confidence: 99%