Repression of Phenol Catabolism by Organic Acids in
            <i>Ralstonia eutropha</i>

Ampe, Frédéric; Leonard, D. R. A.; Lindley, Nic D.

doi:10.1128/aem.64.1.1-6.1998

Cited by 58 publications

(25 citation statements)

References 33 publications

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“…Changes in the distribution of phenol-degrading populations can result from adaptations to phenol degradation or interactions between community members (e.g., predation by protozoans, which were present in both reactors). Mutations in the promoter region of the phenol degradation genes (11), repression by organic acids (4,31), and activities of unknown gene repressors (6) are events that are possible in a mixed community and could affect the fitness of phenol-degrading populations, causing changes in community structure.…”

Section: Discussionmentioning

confidence: 99%

Correspondence between Community Structure and Function during Succession in Phenol- and Phenol-plus-Trichloroethene-Fed Sequencing Batch Reactors

Ayala-del-Rı́o

Callister

Criddle

et al. 2004

Appl Environ Microbiol

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The effects of more than 2 years of trichloroethene (TCE) application on community succession and function were studied in two aerobic sequencing batch reactors. One reactor was fed phenol, and the second reactor was fed both phenol and TCE in sequence twice per day. After initiation of TCE loading in the second reactor, the TCE transformation rates initially decreased, but they stabilized with an average second-order rate coefficient of 0.044 liter mg(-1) day(-1) for 2 years. In contrast, the phenol-fed reactor showed higher and unstable TCE transformation rates, with an average rate coefficient of 0.093 liter mg(-1) day(-1). Community analysis by terminal restriction fragment length polymorphism (T-RFLP) analysis of the 16S rRNA genes showed that the phenol-plus-TCE-fed reactor had marked changes in community structure during the first 100 days and remained relatively stable afterwards, corresponding to the period of stable function. In contrast, the community structure of the phenol-fed reactor changed periodically, and the changes coincided with the periodicity observed in the TCE transformation rates. Correspondence analysis of each reactor community showed that different community structures corresponded with function (TCE degradation rate). Furthermore, the phenol hydroxylase genotypes, as determined by restriction fragment length polymorphism analysis, corresponded to community structure patterns identified by T-RFLP analysis and to periods when the TCE transformation rates were high. Long-term TCE stress appeared to select for a different and stable community structure, with lower but stable TCE degradation rates. In contrast, the community under no stress exhibited a dynamic structure and dynamic function.

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Section: Discussionmentioning

confidence: 99%

Correspondence between Community Structure and Function during Succession in Phenol- and Phenol-plus-Trichloroethene-Fed Sequencing Batch Reactors

Ayala-del-Rı́o

Callister

Criddle

et al. 2004

Appl Environ Microbiol

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“…Cultures were incubated in Erlenmeyer flasks at 307C until complete substrate metabolization in a Gallenkamp (De Mori; Milan, Italy) oscillating shaker (210 rpm). Substrate consumption and product accumulation were monitored hourly by HPLC separation of the culture medium (L-6200A Intelligent Pump equipped with L-4500 diode array detector; Merck Hitachi, Tokyo, Japan) following the method of Ampe et al [15]. The pH variation of the growth medium during culture was also measured.…”

Section: Bacterial Strain and Culture Conditionsmentioning

confidence: 99%

Membrane proteome of Acinetobacter radioresistens S13 during aromatic exposure

Pessione¹,

Giuffrida²,

Prunotto³

et al. 2003

Proteomics

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Study of the bacterial membrane proteome, though in its early stages, is a field of growing interest in the search for information about nutrient transport and processing. We tested different strategies and chemical compounds to extract proteins from the membranes (inner and outer) of Acinetobacter radioresistens S13, a Gram-negative bacterium selected for its ability to degrade aromatics. A. radioresistens S13 was monitored under different growth substrate conditions, using acetate, benzoate or phenol as sole carbon source. Two-dimensional gel electrophoresis map analysis of membrane extracts from benzoate- and phenol-grown cells reveals differences versus controls (acetate-grown cultures). Primarily, a different pattern of spots was observed and, in particular, some proteins were only expressed in the presence of aromatic substrate. Among these, we detected a Na(+)/H(+) antiporter, whose function is likely to be regulation of intracellular pH, and an ABC type sugar transport system, probably involved in capsular polysaccharide translocation. We also identified other proteins, detectable in acetate-grown but over-expressed in aromatic-grown cells. These include: (1) an outer membrane protein ascribable to an OmpA-like protein, recently described in the literature as "alasan", a bioemulsifying agent involved in solubilizing and enhancing bioavailability of hydrocarbons; (2) a trimeric porin of the PhoE family also belonging to the outer membrane and involved in facilitating the transport of anions (especially phosphate); and (3) two glycosyl transferases probably involved in capsules and/or lipopolysaccharide biosynthesis. Study of the bacterial membrane proteome helps to elucidate the role of the membrane as modulable site enabling communication between internal and external environments.

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“…Several examples that binary AC mixtures are hierarchically degraded have been reported, i.e. one AC is a preferred substrate that is catabolized first in the mixture delaying degradation of the second component (Nichols and Harwood, 1995;Ampe et al, 1998;Brzostowicz et al, 2003;Choi et al, 2007;Zhan et al, 2009). The presence of a favourite, preferred substrate whose catabolism represses the expression of genes involved in degradation of the alternative, non-preferred substrate is a phenomenon known as catabolic repression by carbon (CRC).…”

Section: Introductionmentioning

confidence: 99%

Strict and direct transcriptional repression of the pobA gene by benzoate avoids 4‐hydroxybenzoate degradation in the pollutant degrader bacterium Cupriavidus necator JMP134

Donoso

Pérez‐Pantoja²,

González

2011

Environmental Microbiology

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As other environmental bacteria, Cupriavidus necator JMP134 uses benzoate as preferred substrate in mixtures with 4-hydroxybenzoate, strongly inhibiting its degradation. The mechanism underlying this hierarchical use was studied. A C. necator benA mutant, defective in the first step of benzoate degradation, is unable to metabolize 4-hydroxybenzoate when benzoate is also included in the medium, indicating that this substrate and not one of its catabolic intermediates is directly triggering repression. Reverse transcription polymerase chain reaction analysis revealed that 4-hydroxybenzoate 3-hydroxylase-encoding pobA transcripts are nearly absent in presence of benzoate and a fusion of pobA promoter to lacZ reporter confirmed that benzoate drastically decreases the transcription of this gene. Expression of pobA driven by a heterologous promoter in C. necator benA mutant, allows growth on 4-hydroxybenzoate in presence of benzoate, overcoming its repressive effect. In contrast with other bacteria, regulators of benzoate catabolism do not participate in repression of 4-hydroxybenzoate degradation. Moreover, the effect of benzoate on pobA promoter can be observed in heterologous strains with the sole presence of PobR, the transcriptional activator of pobA gene, indicating that PobR is enough to fully reproduce the phenomenon. This novel mechanism for benzoate repression is probably mediated by direct action of benzoate over PobR.

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Repression of Phenol Catabolism by Organic Acids in Ralstonia eutropha

Cited by 58 publications

References 33 publications

Correspondence between Community Structure and Function during Succession in Phenol- and Phenol-plus-Trichloroethene-Fed Sequencing Batch Reactors

Correspondence between Community Structure and Function during Succession in Phenol- and Phenol-plus-Trichloroethene-Fed Sequencing Batch Reactors

Membrane proteome of Acinetobacter radioresistens S13 during aromatic exposure

Strict and direct transcriptional repression of the pobA gene by benzoate avoids 4‐hydroxybenzoate degradation in the pollutant degrader bacterium Cupriavidus necator JMP134

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