Repression of Gurken translation by a meiotic checkpoint in<i>Drosophila</i>oogenesis is suppressed by a reduction in the dose of<i>eIF1A</i>

Li, Wei; Klovstad, Martha; Schüpbach, Trudi

doi:10.1242/dev.109306

Cited by 13 publications

(14 citation statements)

References 55 publications

(80 reference statements)

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“…One hundred sixty starved male flies were collected at each starvation period, quickly frozen in liquid nitrogen, homogenized with a mortar in liquid nitrogen and then polysome buffer was added, as described before [43]. Preparation of the extracts and sucrose gradient separation for the polysome analysis were carried out using a gradient master 107–201M and fractionator 152–002 (BioComp Instruments) by the methods of Inada and Aiba [44].…”

Section: Methodsmentioning

confidence: 99%

The Histone Deacetylase Gene Rpd3 Is Required for Starvation Stress Resistance

Nakajima¹,

Shimaji²,

Umegawachi³

et al. 2016

PLoS ONE

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Epigenetic regulation in starvation is important but not fully understood yet. Here we identified the Rpd3 gene, a Drosophila homolog of histone deacetylase 1, as a critical epigenetic regulator for acquiring starvation stress resistance. Immunostaining analyses of Drosophila fat body revealed that the subcellular localization and levels of Rpd3 dynamically changed responding to starvation stress. In response to starvation stress, the level of Rpd3 rapidly increased, and it accumulated in the nucleolus in what appeared to be foci. These observations suggest that Rpd3 plays a role in regulation of rRNA synthesis in the nucleolus. The RT-qPCR and ChIP-qPCR analyses clarified that Rpd3 binds to the genomic region containing the rRNA promoters and activates rRNA synthesis in response to starvation stress. Polysome analyses revealed that the amount of polysomes was decreased in Rpd3 knockdown flies under starvation stress compared with the control flies. Since the autophagy-related proteins are known to be starvation stress tolerance proteins, we examined autophagy activity, and it was reduced in Rpd3 knockdown flies. Taken together, we conclude that Rpd3 accumulates in the nucleolus in the early stage of starvation, upregulates rRNA synthesis, maintains the polysome amount for translation, and finally increases stress tolerance proteins, such as autophagy-related proteins, to acquire starvation stress resistance.

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Section: Methodsmentioning

confidence: 99%

The Histone Deacetylase Gene Rpd3 Is Required for Starvation Stress Resistance

Nakajima¹,

Shimaji²,

Umegawachi³

et al. 2016

PLoS ONE

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“…The number of eggs laid was counted when the plate was changed and egg phenotypes and fertility rates were assessed 2 days later. Ventralization phenotypes were scored as previously described (Li et al, 2014). …”

Section: Methodsmentioning

confidence: 99%

“…A ventralized egg displaying only one wider, fused dorsal appendage (V2/V3) (Li et al, 2014). A fully ventralized egg displaying no dorsal appendages (V4).…”

Section: Figurementioning

confidence: 99%

Zfrp8 forms a complex with fragile-X mental retardation protein and regulates its localization and function

Tan

Schauder

Naryshkina

et al. 2016

Developmental Biology

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Fragile-X syndrome is the most commonly inherited cause of autism and mental disabilities. The Fmr1 (Fragile-X Mental Retardation 1) gene is essential in humans and Drosophila for the maintenance of neural stem cells, and Fmr1 loss results in neurological and reproductive developmental defects in humans and flies. FMRP (Fragile-X Mental Retardation Protein) is a nucleo-cytoplasmic shuttling protein, involved in mRNA silencing and translational repression. Both Zfrp8 and Fmr1 have essential functions in the Drosophila ovary. In this study, we identified FMRP, Nufip (Nuclear Fragile-X Mental Retardation Protein-interacting Protein) and Tral (Trailer Hitch) as components of a Zfrp8 protein complex. We show that Zfrp8 is required in the nucleus, and controls localization of FMRP in the cytoplasm. In addition, we demonstrate that Zfrp8 genetically interacts with Fmr1 and tral in an antagonistic manner. Zfrp8 and FMRP both control heterochromatin packaging, also in opposite ways. We propose that Zfrp8 functions as a chaperone, controlling protein complexes involved in RNA processing in the nucleus.

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“…56 Another component shown to be involved in the control of grk translation is the heterogeneous nuclear ribonucleoprotein (hnRNP) Squid (Sqd). [58][59][60] Sqd and Cup have been shown to interact through both biochemical and genetic analysis where cup mutants enhance the dorsalised phenotype of sqd homozygotes. 56 However, whether this enhancement of sqd by cup is specifically due to loss of function of Cup independent of the role in the orb auto-regulatory loop remains to be fully investigated.…”

Section: Cpe-mediated Translational Controlmentioning

confidence: 99%

Translational control ofgurkenmRNA inDrosophiladevelopment

Derrick

Weil

2016

Cell Cycle

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Localized mRNA translation is a widespread mechanism for targeting protein synthesis, important for cell fate, motility and pathogenesis. In Drosophila, the spatiotemporal control of gurken/TGF-a mRNA translation is required for establishing the embryonic body axes. A number of recent studies have highlighted key aspects of the mechanism of gurken mRNA translational control at the dorsoanterior corner of the mid-stage oocyte. Orb/CPEB and Wispy/GLD-2 are required for polyadenylation of gurken mRNA, but unlocalized gurken mRNA in the oocyte is not fully polyadenylated.1 At the dorsoanterior corner, Orb and gurken mRNA have been shown to be enriched at the edge of Processing bodies, where translation occurs.2 Over-expression of Orb in the adjacent nurse cells, where gurken mRNA is transcribed, is sufficient to cause mis-expression of Gurken protein.3 In orb mutant egg chambers, reducing the activity of CK2, a Serine/Threonine protein kinase, enhances the ventralized phenotype, consistent with perturbation of gurken translation. 4 Here we show that sites phosphorylated by CK2 overlap with active Orb and with Gurken protein expression. Together with our new findings we consolidate the literature into a working model for gurken mRNA translational control and review the role of kinases, cell cycle factors and polyadenylation machinery highlighting a multitude of conserved factors and mechanisms in the Drosophila egg chamber.

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Repression of Gurken translation by a meiotic checkpoint inDrosophilaoogenesis is suppressed by a reduction in the dose ofeIF1A

Cited by 13 publications

References 55 publications

The Histone Deacetylase Gene Rpd3 Is Required for Starvation Stress Resistance

The Histone Deacetylase Gene Rpd3 Is Required for Starvation Stress Resistance

Zfrp8 forms a complex with fragile-X mental retardation protein and regulates its localization and function

Translational control ofgurkenmRNA inDrosophiladevelopment

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