Reporting long-term effects of metabolic stress on human ovary carcinoma

Nakhjavani, Maryam; Shirazi, Farshad H.

doi:10.12991/mpj.2018.52

Cited by 2 publications

(2 citation statements)

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“…The antiproliferative activity of all durian rind extracts was assessed against HepG‐2 and MCF‐7 cell lines using the Sulforhodamine B (SRB) assay, following a previously established protocol (Nakhjavani et al ., 2014). Cells were seeded at 5000 cells/well in 96‐well plates and serial dilutions of the extracts (50–500 μg mL −1 ) were prepared.…”

Section: Methodsmentioning

confidence: 99%

Extraction of flavonoids from durian (Durio zibethinus) fruit rinds and evaluation of their antioxidant, antidiabetic and anticancer properties

Tran,

Nguyen,

Nguyen

et al. 2023

Int J of Food Sci Tech

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SummaryDurian (Durio zibethinus) fruit rinds are a promising source of bioactive phytochemicals, but they are currently wasted. This study optimised extraction conditions for flavonoids from durian fruit rinds and evaluated the biological activities of the flavonoid‐rich extracts. The highest flavonoid content of 82.17 mg QE g−1 extract was achieved when performing the extraction at 60 °C using 75% ethanol and maceration time of 24 h. The crude ethanolic extract was then fractionated using liquid–liquid extraction to obtain three fractions (CHF, EAF and AQF). Among them, CHF demonstrated the highest amount of flavonoids (271.11 mg QE g−1 extract) and quercetin (1006.19 μg g−1 extract). This fraction also exhibited the highest ferric reducing power (IC50 of 307.88 μg vitamin C g−1), DPPH radical scavenging activity (IC50 of 38.72 μg mL−1), α‐amylase and α‐glucosidase inhibitory activity (IC50 of 685.76 μg mL−1 and 441.10 μg mL−1, respectively) and cytotoxicity against HepG‐2 and MCF‐7 cells with IC50 values of 490.30 μg mL−1 and 343.38 μg mL−1 respectively. The current work suggested that durian fruit rinds are a potential source of flavonoids and the CHF could be further used for pharmaceutical applications.

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Section: Methodsmentioning

confidence: 99%

Extraction of flavonoids from durian (Durio zibethinus) fruit rinds and evaluation of their antioxidant, antidiabetic and anticancer properties

Tran,

Nguyen,

Nguyen

et al. 2023

Int J of Food Sci Tech

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“…In our previous research, we studied human lung carcinoma cell lines' reaction against metabolic stress. [21][22][23] To the best of authors' knowledge, this is the first time that this cancer cell line's behaviour at different metabolic states and its ability to come back to life following metabolic stress is studied.…”

Section: Introductionmentioning

confidence: 99%

Long-Term Effects of Metabolic Stress on Human Malignant Melanoma Cell Line

Nakhjavani¹,

Shirazi²

2018

IJPER

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Introduction: Tumour is a heterogeneous tissue consisting of cells with different levels of metabolic activity. Often the outer layer cells of a tumour have more optimal conditions to grow/proliferate compared to the inner cells. Objective: The aim of the current study was to study the reactions of malignant melanoma A375 cells in exposure to different levels of metabolic stress and their ability to returning to life upon re-exposure to optimum nutritional conditions. Methods: A375 cells, at early plateau phase, were exposed to media containing 10% (control), 0.5, 0.25 and 0% serum for 1 to 6 days. At 24 h intervals, the cells were tested for morphology, cell cycle distribution, cell size changes, cell count, mitochondrial function and protein content. Also, after each day of starvation, the cells were re-exposed to optimum media (10% serum) and tested again. Results: The results showed that the cells made foci structures at long-term starvation. They primarily accumulated in G1-phase and at long-term starvation, most of them entered the apoptotic state. However, before death, cell count did not decrease, even though the mitochondrial function and protein content were less than the control cells. Releasing the cells in optimum conditions could trigger cells' proliferation ability, mitochondrial function and protein content. Conclusion: The results suggest that this model of malignant melanoma has a resistant feature against sub-optimal nutritional and metabolic status and the cells can return to life with even stronger internal engines, as evidenced by higher levels of mitochondrial function and protein content.

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Reporting long-term effects of metabolic stress on human ovary carcinoma

Cited by 2 publications

References 0 publications

Extraction of flavonoids from durian (Durio zibethinus) fruit rinds and evaluation of their antioxidant, antidiabetic and anticancer properties

Extraction of flavonoids from durian (Durio zibethinus) fruit rinds and evaluation of their antioxidant, antidiabetic and anticancer properties

Long-Term Effects of Metabolic Stress on Human Malignant Melanoma Cell Line

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