Reporter Genes for Brain Imaging Using MRI, SPECT and PET

Gao, Tianxin; Wang, Pei; Gong, Teng; Zhou, Ying; Wang, Ancong; Tang, Xiaoying; Song, Xiaolei; Fan, Yingwei

doi:10.3390/ijms23158443

Cited by 6 publications

(4 citation statements)

References 91 publications

(137 reference statements)

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“…111,112 The main brain imaging techniques that can be used to track cells in vivo are MRI, X-ray computed tomography (CT), positron emission tomography (PET), and single-photon emission computed tomography (SPECT). [113][114][115] However, these techniques usually require appropriate contrast agents to label transplanted cells to enhance contrast. Nanomaterials are promising candidates for contrast agents because of their unique optical properties, cellular uptake, surface functionalization, and good biocompatibility.…”

Section: Nanoparticle-based Stem Cell Labeling and Trackingmentioning

confidence: 99%

Nanotherapeutic and Stem Cell Therapeutic Strategies in Neurodegenerative Diseases: A Promising Therapeutic Approach

Wei¹,

Yang²,

Li³

2023

IJN

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Neurodegeneration is characterized by progressive, disabling, and incurable neurological disorders with the massive loss of specific neurons. As one of the most promising potential therapeutic strategies for neurodegenerative diseases, stem cell therapy exerts beneficial effects through different mechanisms, such as direct replacement of damaged or lost cells, secretion of neurotrophic and growth factors, decreased neuroinflammation, and activation of endogenous stem cells. However, poor survival and differentiation rates of transplanted stem cells, insufficient homing ability, and difficulty tracking after transplantation limit their further clinical use. The rapid development of nanotechnology provides many promising nanomaterials for biomedical applications, which already have many applications in neurodegenerative disease treatment and seem to be able to compensate for some of the deficiencies in stem cell therapy, such as transport of stem cells/genes/drugs, regulating stem cell differentiation, and real-time tracking in stem cell therapy. Therefore, nanotherapeutic strategies combined with stem cell therapy is a promising therapeutic approach to treating neurodegenerative diseases. The present review systematically summarizes recent advances in stem cell therapeutics and nanotherapeutic strategies and highlights how they can be combined to improve therapeutic efficacy for the treatment of neurodegenerative diseases.

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Section: Nanoparticle-based Stem Cell Labeling and Trackingmentioning

confidence: 99%

Nanotherapeutic and Stem Cell Therapeutic Strategies in Neurodegenerative Diseases: A Promising Therapeutic Approach

Wei¹,

Yang²,

Li³

2023

IJN

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“…MRI reporter genes contain a specific cellular receptor, an enzyme coding gene, and an endogenous reporter gene ( 68 ). The advantages of this reporter gene are that the signal does not weaken with cell division and the reporter gene is only expressed in viable cells, making it possible to track target cells in vivo indefinitely.…”

Section: Indirect Cell Labeling Methodsmentioning

confidence: 99%

In Vivo Stem Cell Imaging Principles and Applications

Hong,

Lee,

Bae

et al. 2023

Int J Stem Cells

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Stem cells are the foundational cells for every organ and tissue in our body. Cell-based therapeutics using stem cells in regenerative medicine have received attracting attention as a possible treatment for various diseases caused by congenital defects. Stem cells such as induced pluripotent stem cells (iPSCs) as well as embryonic stem cells (ESCs), mesenchymal stem cells (MSCs), and neuroprogenitors stem cells (NSCs) have recently been studied in various ways as a cell-based therapeutic agent. When various stem cells are transplanted into a living body, they can differentiate and perform complex functions. For stem cell transplantation, it is essential to determine the suitability of the stem cell-based treatment by evaluating the origin of stem, the route of administration, in vivo bio-distribution, transplanted cell survival, function, and mobility. Currently, these various stem cells are being imaged in vivo through various molecular imaging methods. Various imaging modalities such as optical imaging, magnetic resonance imaging (MRI), ultrasound (US), positron emission tomography (PET), and single-photon emission computed tomography (SPECT) have been introduced for the application of various stem cell imaging. In this review, we discuss the principles and recent advances of in vivo molecular imaging for application of stem cell research.

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“…Molecular imaging technology in brain research can aid in diagnosis and treatment but can also be used to study brain function. At the molecular level, optical imaging has also been used with the support of reporter gene technology [ 2 ]. Machine learning (ML) has been implemented for cancer classification, diagnosis, genomic biomarker identification, progression detection, and survival prediction.…”

Section: Introductionmentioning

confidence: 99%

Multiple Cell Cultures for MRI Analysis

Bober

Aebisher²,

Olek³

et al. 2022

IJMS

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Magnetic resonance imaging (MRI) is an imaging method that enables diagnostics. In recent years, this technique has been widely used for research using cell cultures used in pharmaceutical science to understand the distribution of various drugs in a variety of biological samples, from cellular models to tissues. MRI’s dynamic development in recent years, in addition to diagnostics, has allowed the method to be implemented to assess response to applied therapies. Conventional MRI imaging provides anatomical and pathological information. Due to advanced technology, MRI provides physiological information. The use of cell cultures is very important in the process of testing new synthesized drugs, cancer research, and stem cell research, among others. Two-dimensional (2D) cell cultures conducted under laboratory conditions, although they provide a lot of information, do not reflect the basic characteristics of the tumor. To replicate the tumor microenvironment in science, a three-dimensional (3D) culture of tumor cells was developed. This makes it possible to reproduce in vivo conditions where, in addition, there is a complex and dynamic process of cell-to-cell communication and cell–matrix interaction. In this work, we reviewed current research in 2D and 3D cultures and their use in MRI studies. Articles for each section were collected from PubMed, ScienceDirect, Web of Science, and Google Scholar.

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Reporter Genes for Brain Imaging Using MRI, SPECT and PET

Cited by 6 publications

References 91 publications

Nanotherapeutic and Stem Cell Therapeutic Strategies in Neurodegenerative Diseases: A Promising Therapeutic Approach

Nanotherapeutic and Stem Cell Therapeutic Strategies in Neurodegenerative Diseases: A Promising Therapeutic Approach

In Vivo Stem Cell Imaging Principles and Applications

Multiple Cell Cultures for MRI Analysis

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