Reporter gene imaging using radiographic contrast from nonradioactive iodide sequestered by the sodium–iodide symporter

Brown, Stephen L.; Freytag, Svend O.; Barton, Kenneth; Flynn, Michael J.; Peck, Donald J.; Dragovic, Aleksandar F.; Jin, Ryan; Yeni, Yener N.; Fyhrie, David P.; Les, Clifford M.; Zhu, Guopei; Kolozsvary, Andrew; Pitchford, W. C.; Nathanson, David; Fenstermacher, Joseph D.; Kim, Jae Ho

doi:10.1002/cmmi.150

Cited by 4 publications

(4 citation statements)

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“…An array of iodine concentrations found in human tumor breast tissue could be analyzed, although determining this, or even some average or maximum concentration may be challenging and requires thorough validation. Another potential study could use animal models, similar to that done by Brown et al [14], implanting the animals with an array NIS expressing breast tumor cell lines and imaging them. Instead of transducing the cells with an NIS expressing adenovirus, this study would analyze the level of naturally occurring iodine.…”

Section: Experimental Evaluation Of the Hypothesismentioning

confidence: 96%

“…It has furthermore been shown that iodine collecting in breast tumor cells may be sufficient to result in a noticeable radiographic contrast. Brown et al grew human cancer cell tumors in the gastrocnemius muscles of mice, which were then transduced with an NIS expressing adenovirus [14]. Radiographs of these mice showed observable radiographic contrast in areas exhibiting an enhanced concentration of iodine, verified through tissue sections [14].…”

Section: Physical Basismentioning

confidence: 98%

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The potential of iodine for improving breast cancer diagnosis and treatment

et al. 2013

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Section: Experimental Evaluation Of the Hypothesismentioning

confidence: 96%

Section: Physical Basismentioning

confidence: 98%

The potential of iodine for improving breast cancer diagnosis and treatment

et al. 2013

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“…The human sodium iodide symporter can catalyze uptake of negatively charged ions like iodide, technetium etc. and has several substrates that can be detected using CT, PET or SPECT imaging [ 122 , 123 ]. Following intraprostatic injection of the Ad5-yCD/ mut TK SR39 rep -hNIS adenoviral vector in men, non-invasive imaging of NIS gene expression by Na 99m TcO 4 uptake in cells infected with the vector was performed by SPECT.…”

Section: Gene Therapy Clinical Trials At Henry Ford Healthmentioning

confidence: 99%

Oncolytic virus-based suicide gene therapy for cancer treatment: a perspective of the clinical trials conducted at Henry Ford Health

et al. 2023

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Gene therapy manipulates or modifies a gene that provides a new cellular function to treat or correct a pathological condition, such as cancer. The approach of using gene manipulation to modify patient’s cells to improve cancer therapy and potentially find a cure is gaining popularity. Currently, there are 12 gene therapy products approved by US-FDA, EMA and CFDA for cancer management, these include Rexin-G, Gendicine, Oncorine, Provange among other. The Radiation Biology Research group at Henry Ford Health has been actively developing gene therapy approaches for improving clinical outcome in cancer patients. The team was the first to test a replication-competent oncolytic virus armed with a therapeutic gene in humans, to combine this approach with radiation in humans, and to image replication-competent adenoviral gene expression/activity in humans. The adenoviral gene therapy products developed at Henry Ford Health have been evaluated in more than 6 preclinical studies and evaluated in 9 investigator initiated clinical trials treating more than100 patients. Two phase I clinical trials are currently following patients long term and a phase I trial for recurrent glioma was initiated in November 2022. This systematic review provides an overview of gene therapy approaches and products employed for treating cancer patients including the products developed at Henry Ford Health.

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“…μ CT imaging has also been employed to image the expression of sodium-iodide symporter (a reporter gene) in mouse tumors. 54 Perfusion DSA has been employed to investigate vasculature flow patterns. 55,56 Pulmonary blood volume, blood flow, and mean transit times can be calculated from DSA images 4,7,56 (see Figure 2).…”

Section: Computed Tomographymentioning

confidence: 99%

Whole animal imaging

Sandhu

Solorio

Broome

et al. 2010

WIREs Mechanisms of Disease

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Translational research plays a vital role in understanding the underlying pathophysiology of human diseases, and hence development of new diagnostic and therapeutic options for their management. After creating an animal disease model, pathophysiologic changes and effects of a therapeutic intervention on them are often evaluated on the animals using immunohistologic or imaging techniques. In contrast to the immunohistologic techniques, the imaging techniques are noninvasive and hence can be used to investigate the whole animal, oftentimes in a single exam which provides opportunities to perform longitudinal studies and dynamic imaging of the same subject, and hence minimizes the experimental variability, requirement for the number of animals, and the time to perform a given experiment. Whole animal imaging can be performed by a number of techniques including x-ray computed tomography, magnetic resonance imaging, ultrasound imaging, positron emission tomography, single photon emission computed tomography, fluorescence imaging, and bioluminescence imaging, among others. Individual imaging techniques provide different kinds of information regarding the structure, metabolism, and physiology of the animal. Each technique has its own strengths and weaknesses, and none serves every purpose of image acquisition from all regions of an animal. In this review, a broad overview of basic principles, available contrast mechanisms, applications, challenges, and future prospects of many imaging techniques employed for whole animal imaging is provided. Our main goal is to briefly describe the current state of art to researchers and advanced students with a strong background in the field of animal research.

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Reporter gene imaging using radiographic contrast from nonradioactive iodide sequestered by the sodium–iodide symporter

Cited by 4 publications

References 16 publications

The potential of iodine for improving breast cancer diagnosis and treatment

The potential of iodine for improving breast cancer diagnosis and treatment

Oncolytic virus-based suicide gene therapy for cancer treatment: a perspective of the clinical trials conducted at Henry Ford Health

Whole animal imaging

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