Report on the 13th International Society of Blood Transfusion Platelet Immunology Workshop

Foxcroft, Zyta Krystyna; Campbell, Kate; Mérieux, Yves; Urbaniak, S. J.; Brierley, Matthew; Rigal, Dominique; Ouwehand, Willem H.; Metcalfe, Paul

doi:10.1111/j.1423-0410.2007.00951.x

Cited by 11 publications

(11 citation statements)

References 7 publications

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“…The efforts to rule out HPA‐15 alloantibodies and to standardise an efficient detection technique should be strong because of its clinical relevance. The report on the 13th International Society of Blood Transfusion Platelet Immunology Workshop highlighted the persistence of variability between laboratories even though it has been reduced compared to previous years (Foxcroft et al ., 2007).…”

Section: Discussionmentioning

confidence: 99%

Severe neonatal alloimmune thrombocytopaenia with anaemia

Novelli

Canals

Nogués

et al. 2010

Transfusion Medicine

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Section: Discussionmentioning

confidence: 99%

Severe neonatal alloimmune thrombocytopaenia with anaemia

Novelli

Canals

Nogués

et al. 2010

Transfusion Medicine

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“…In another article in this edition, Foxcroft et al . [7] report the results of an international workshop exercise where an identical, shortened MAIPA assay was used by all participants and yet different results were still obtained. This would seem to indicate that the differences in reactivity are due to a combination of factors, each of which individually has little or no effect, or are due to laboratory expertise.…”

Section: Discussionmentioning

confidence: 99%

Interlaboratory variation in the detection of HPA‐specific alloantibodies and in molecular HPA typing

Allen¹,

Ouwehand

Haas

et al. 2007

Vox Sanguinis

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The ability to detect and to identify platelet-specific alloantibodies varied widely between laboratories and between various examples of antibodies issued. An increase in the number of laboratories screening for HPA-15 antibodies was seen, although detection and identification of these antibodies was problematic. The majority of examples of HPA-3a antibodies and some examples of HPA-1a and -5b were also difficult to detect and identify. In addition, this scheme has shown that despite the apparent reliability of molecular typing techniques, mistakes do occur, particularly with certain systems. Approximately one in five laboratories participating in the serology exercises and one in seven participating in the genotyping exercises were classified as poor performer at one point or more during the series of exercises.

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“…Methods for the desiccation and dry storage of human cells (RBCs, platelets, stem cells, and gametes) are not still available for clinical applications [28, 29]. However, freeze-dried cells have been used for some diagnostic applications [30, 31] and as biological controls for cell counting in blood cell analyzers and for flow cytometry assays [32]. …”

Section: Introductionmentioning

confidence: 99%

Stabilization of Transfected Cells Expressing Low-Incidence Blood Group Antigens: Novel Methods Facilitating Their Use as Reagent-Cells

et al. 2016

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BackgroundThe identification of erythrocyte antibodies in the serum of patients rely on panels of human red blood cells (RBCs), which coexpress many antigens and are not easily available for low-incidence blood group phenotypes. These problems have been addressed by generating cell lines expressing unique blood group antigens, which may be used as an alternative to human RBCs. However, the use of cell lines implies several drawbacks, like the requirement of cell culture facilities and the high cost of cryopreservation. The application of cell stabilization methods could facilitate their use as reagent cells in clinical laboratories.MethodsWe generated stably-transfected cells expressing low-incidence blood group antigens (Dia and Lua). High-expresser clones were used to assess the effect of TransFix® treatment and lyophilization as cell preservation methods. Cells were kept at 4°C and cell morphology, membrane permeability and antigenic properties were evaluated at several time-points after treatment.ResultsTransFix® addition to cell suspensions allows cell stabilization and proper antigen detection for at least 120 days, despite an increase in membrane permeability and a reduction in antigen expression levels. Lyophilized cells showed minor morphological changes and antigen expression levels were rather conserved at days 1, 15 and 120, indicating a high stability of the freeze-dried product. These stabilized cells have been proved to react specifically with human sera containing alloantibodies.ConclusionsBoth stabilization methods allow long-term preservation of the transfected cells antigenic properties and may facilitate their distribution and use as reagent-cells expressing low-incidence antigens, overcoming the limited availability of such rare RBCs.

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Report on the 13th International Society of Blood Transfusion Platelet Immunology Workshop

Cited by 11 publications

References 7 publications

Severe neonatal alloimmune thrombocytopaenia with anaemia

Severe neonatal alloimmune thrombocytopaenia with anaemia

Interlaboratory variation in the detection of HPA‐specific alloantibodies and in molecular HPA typing

Stabilization of Transfected Cells Expressing Low-Incidence Blood Group Antigens: Novel Methods Facilitating Their Use as Reagent-Cells

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