Report of the Texas peanut root-knot nematode, Meloidogyne haplanaria (Tylenchida: Meloidogynidae) from American pitcher plants (Sarracenia sp.) in California

Abstract

During the winter and spring of 2021, the root-knot nematodes were extracted from samples of galled roots of potted American pitcher plants (Sarracenia sp.). Samples were collected from a botanical garden nursery in Los Angeles County, California. The root-knot nematode was identified by molecular methods as Meloidogyne haplanaria. In the USA, M. haplanaria was initially found in Texas, and subsequently reported from Arkansas and Florida. Molecular characterization of the Californian M. haplanaria isolate was done using the analysis of the D2-D3 of 28S rRNA, ITS rRNA, mitochondrial l-rRNA, COI, and nad5 gene sequences. Some rRNA gene clusters of M. haplanaria were similar with those of M. arenaria. Possible hybridization events within mitotic parthenogenetic root-knot nematodes are discussed. This study confirmed that reliable diagnostics of M. haplanaria should be based on mtDNA sequence analysis. This is a first report of M. haplanaria from Sarracenia sp. and California. Consequently, this nematode was considered to be eradicated from this botanical garden nursery and the State of California.

First report of barley root-knot nematode, Meloidogyne naasi from turfgrass in Idaho, with multigene molecular characterization

“…Molecular markers continue to be a useful tool for distinguishing Meloidogyne species, particularly ribosomal large subunit (LSU) D2-D3 segments of the 28S rDNA (Skantar et al, 2021;Trinh et al, 2022;Zeng et al, 2022) and the mitochondrial DNA interval that spans the region between genes encoding cytochrome oxidase subunit II (COII) and 16S rRNA (Powers and Harris, 1993;Subbotin, 2021;Subbotin and Burbridge, 2021). IGS-2 has been sequenced from several RKN species, but not from M. naasi.…”