Report of the First International Workshop on Equine Leucocyte Antigens, Cambridge, UK, July 1991

Kydd, Julia H.; Antczak, Douglas F.; Allen, W. R.; Barbis, Dina P.; Butcher, Geoffrey W.; Davis, William C.; Duffus, W. P. H.; Edington, N.; Grünig, Gabriele; Holmes, Mark A.; Lunn, D.P.; McCulloch, J.; O'Brien, A D; Perryman, Lance E.; Tavernor, Angela S.; Williamson, Stanley; Zhang, C.

doi:10.1016/0165-2427(94)90088-4

Cited by 85 publications

(58 citation statements)

References 38 publications

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“…Embora existam relatos de imunofenotipagem de linfócitos no sangue periférico de eqüinos adultos (LUNN et al, 1991;BLANCHARD-CHANNEL et al, 1994;KYDD et al, 1994;AKENS et al, 1997;BENDALI-AHCÈNE et al, 1995;1997) BENDALI-AHCÈNE et al (1997).…”

Section: Discussionunclassified

“…Estudos fazem referência à imunofenotipagem de linfócitos do sangue periférico de eqüinos (LUNN et al, 1991;BLANCHARD-CHANNEL et al, 1994;KYDD et al, 1994;AKENS et al, 1997;BENDALI-AHCÈNE et al, 1997;LUNN et al, 1998). Entretanto, as características imunofenotípicas das células linfocitárias do SCU da referida espécie ainda não foram avaliadas.…”

Section: Introductionunclassified

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Quantificação de subpopulações linfocitárias no sangue do cordão umbilical de eqüinos

et al. 2007

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Section: Discussionunclassified

Section: Introductionunclassified

Quantificação de subpopulações linfocitárias no sangue do cordão umbilical de eqüinos

et al. 2007

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“…For the analysis of cell phenotype, a total of 10 6 cells were stimulated in vitro with sAgs at the indicated concentrations or medium alone as negative controls. After 3 days of culture at 37°C, surface labeling was performed with antibodies that recognize equine CD4 (clone HB61A), equine CD5 (clone HB19a; CD5 is not expressed on equine B lymphocytes [12,18]), and equine CD8␣ (clone 73/6.9.1), used at 1 g per 10 6 PBMC. Control isotype antibodies were obtained from DakoCytomation (Glostrup, Denmark).…”

Section: Methodsmentioning

confidence: 99%

Contribution of Each of Four Superantigens toStreptococcus equi-Induced Mitogenicity, Gamma Interferon Synthesis, and Immunity

et al. 2010

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Streptococcus equi is the causative agent of strangles, the most frequently diagnosed infectious disease of horses worldwide. The disease is characterized by abscessation and swelling of the lymph nodes of the head and neck, which can literally strangle the horse to death. S. equi produces four recently acquired phage-associated bacterial superantigens (sAgs; SeeH, SeeI, SeeL, and SeeM) that share homology with the mitogenic toxins of Streptococcus pyogenes. The aim of this study was to characterize the contribution of each of these S. equi sAgs to mitogenic activity in vitro and quantify the sAg-neutralizing capacity of sera from naturally infected horses in order to better understand their role in pathogenicity. Each of the sAgs was successfully cloned, and soluble proteins were produced in Escherichia coli. SeeI, SeeL, and SeeM induced a dose-dependent proliferative response in equine CD4 T lymphocytes and synthesis of gamma interferon (IFN-␥). SeeH did not stimulate equine peripheral blood mononuclear cells (PBMC) but induced proliferation of asinine PBMC. Allelic replacement mutants of S. equi strain 4047 with sequential deletion of the superantigen genes were generated. Deletion of seeI, seeL, and seeM completely abrogated the mitogenic activity and synthesis of IFN-␥, in equine PBMC, of the strain 4047 culture supernatant. Sera from naturally infected convalescent horses had only limited sAg-neutralizing activities. We propose that S. equi sAgs play an important role in S. equi pathogenicity by stimulating an overzealous and inappropriate Th1 response that may interfere with the development of an effective immune response.

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“…Homologous and heterologous target cells for these assays were EIAV WSU5 -infected and noninfected EK cells (3). Fluorescent flow cytometry was performed (25) using the following murine anti-equine monoclonal antibodies: ETC91A (25) and HT14A (anti-CD8); HB61A (anti-CD4); and F66 (anti-CD3) (26).…”

Section: Preparation Of Viral-specific Lymphocytesmentioning

confidence: 99%

Immune Reconstitution Prevents Continuous Equine Infectious Anemia Virus Replication in an Arabian Foal with Severe Combined Immunodeficiency: Lessons for Control of Lentiviruses

Mealey

Fraser

Oaks

et al. 2001

Clinical Immunology

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Acute infection with equine infectious anemia virus (EIAV), a lentivirus of horses, results in a persistent high-level viremia in Arabian foals affected with severe combined immunodeficiency (SCID). This observation argues against the idea that the transient nature of acute lentiviral viremia is solely a function of viral population dynamics. To extend these studies, EIAV-specific immune reconstitution was attempted prior to EIAV challenge in 2 SCID foals, using adoptively transferred virus-stimulated lymphocytes derived from persistently EIAV-infected half sibling donors. Following transfer, lymphocyte engraftment occurred in 1 foal, and EIAV-specific cytotoxic T lymphocytes as well as neutralizing antibody activity developed. Following a brief period of plasma viremia in this foal, EIAV replication was controlled and plasma virus could not be detected by RT-PCR or culture. These results provide further direct evidence that a specific immune response is required for termination of plasma viremia in acute lentiviral infections.

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Report of the First International Workshop on Equine Leucocyte Antigens, Cambridge, UK, July 1991

Cited by 85 publications

References 38 publications

Quantificação de subpopulações linfocitárias no sangue do cordão umbilical de eqüinos

Quantificação de subpopulações linfocitárias no sangue do cordão umbilical de eqüinos

Contribution of Each of Four Superantigens toStreptococcus equi-Induced Mitogenicity, Gamma Interferon Synthesis, and Immunity

Immune Reconstitution Prevents Continuous Equine Infectious Anemia Virus Replication in an Arabian Foal with Severe Combined Immunodeficiency: Lessons for Control of Lentiviruses

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