Report of the Committee on the Genetic Constitution of the X Chromosome

Ps, Gerald; Ja, Brown

doi:10.1159/000130228

Cited by 29 publications

(11 citation statements)

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“…Ricciuti & Ruddle (63, 64) using hybrids between mouse cells and a human cell line carrying an X-14 translocation ft(l4q-Xq)] showed that Gpd could be assigned to Xq. A number of subsequent studies confi rmed this and further localized Gpd distal to band Xq26 (305,306).…”

Section: 122)mentioning

confidence: 72%

“…Further studies with somatic cell hybrids using human cell lines with transloca tions involving the X chromosome have localized Pgk to bands Xq 12-Xq21 (305,306).…”

Section: 122)mentioning

confidence: 99%

“…Hp rt was localized to the long arm of the X chromosome by Ricciuti & Ruddle (63, 64) using hybrids between mouse cells and human cells with an X-14 translocation. Sub sequent studies have indicated that Hp rt can be assigned to bands Xq24-Xq26 (305,306).…”

Section: 122)mentioning

confidence: 99%

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Parasexual Approaches to the Genetics of Man

1975

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Section: 122)mentioning

confidence: 72%

“…Further studies with somatic cell hybrids using human cell lines with transloca tions involving the X chromosome have localized Pgk to bands Xq 12-Xq21 (305,306).…”

Section: 122)mentioning

confidence: 99%

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Parasexual Approaches to the Genetics of Man

1975

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“…These conflicting reports demonstrated a need to use different X-autosome translocations to determine the X chromosome map. P. S. Gerald and G. Bruns (personal communication), employing cells with an X/19 translocation, have cell hybrid segregation data to support the assignment of HPRT and G6PD to the distal half (q24-qter) of the X long arm (28). Pearson et al (31), in a preliminary report, have utilized cells with an X/22 (Breda) translocation, which also supports the assignment of HPRT and G6PD to the distal half (q23-qter) of the X long arm.…”

Section: Resultsmentioning

confidence: 95%

“…One hundred-A. X/9 CHROMOSOME MARKERS (11,28). For nonselected linkage groups, 4-6% discordancy was observed in these hybrids (11,26).…”

Section: Resultsmentioning

confidence: 97%

Human X-Linked genes regionally mapped utilizing X-autosome translocations and somatic cell hybrids.

Shows¹,

Brown²

1975

Proc. Natl. Acad. Sci. U.S.A.

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Human genes coding for hypoxanthine phosphoribosyltransferase (HPRT, EC 2.4.2.8; IMP:pyrophosphate phosphoribosyltransferase), glucose-6-phosphate dehydrogenase (G6PD, EC 1.1.1.49; D-glucose-6-phosphate: NADP+ 1-oxidoreductase), and phosphoglycerate kinase (PGK, EC 2.7.2.3; ATP:3-phospho-D-glycerate 1-phosphotransferase) have been assigned to specific regions on the long arm of the X chromosome by somatic cell genetic techniques. Gene assignment and linear order were determined by employing human somatic cells possessing an X/9 translocation or an X/22 translocation in man-mouse cell hybridization studies. The X/9 translocation involved the majority of the X long arm translocated to chromosome 9 and the X/22 translocation involved the distal half of the X long arm translocated to 22. In each case these rearrangements appeared to be reciprocal. Concordant segregation of X-linked enzymes and segments of the X chromosome generated by the translocations indicated assignment of the PGK gene to a proximal long arm region (q12-q22) and the HPRT and G6PD genes to the distal half (q22-qter) of the X long arm. Further evidence suggests a gene order on the X long arm of centromere-PGK-HPRT-G6PD.Man-rodent somatic cell hybrids have proved important for mapping the human genome (1, 2). Human genes can be assigned to specific chromosomes, since human chromosomes are preferentially lost in these cell hybrids. By employing cells with deleted or translocated human chromosomes in cell hybrid studies, one can determine gene order and assignment to a specific chromosomal region (3-6). The human X chromosome is of interest to map, since it is involved in sex determination; it is functionally inactivated in females, resulting in the same gene dosage as in males (7); and X-linked genes are involved in several inherited metabolic defects.We employed two inherited X-autosome translocations and somatic cell hybrids to map genes coding for hypoxanthine phosphoribosyltransferase (HPRT, EC 2.4.2.8; IMP: pyrophosphate phosphoribosyltransferase), glucose-6-phosphate dehydrogenase (G6PD, EC 1.1.1.49; D-glucose-6-phosphate: NADP+ 1-oxidoreductase), and phosphoglycerate kinase (PGK, EC 2.7.2.3; ATP:3-phospho-D-glycerate 1-phosphotransferase) to specific regions of the X long arm. These three genes have been shown to be X-linked by family and somatic cell genetic studies (3,(8)(9)(10)(11)(12), and enzyme deficiencies of HPRT, G6PD, and PGK in man have been associated, respectively, with Lesch-Nyhan disease (8), drug-induced hemolytic anemia (13), and chronic hemolytic anemia (14).Abbreviations: HPRT, hypoxanthine phosphoribosyl transferase; G6PD, glucose-6-phosphate dehydrogenase; PGK, phosphoglycerate kinase; HAT, hypoxanthine/aminopterin/thymidine; DMEM, Dulbecco's modified Eagle's medium. 2125Mapping the X chromosome in cell hybrids is aided by a system to select for or against hybrid cells possessing human HRPT (15). This procedure, in concert with an X/9 and an X/22 translocation involving different segments of the X long arm translo...

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Gene Localizations and Other Properties of Human Chromosomes

King

1975

Handbook of Genetics

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Report of the Committee on the Genetic Constitution of the X Chromosome

Cited by 29 publications

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Parasexual Approaches to the Genetics of Man

Parasexual Approaches to the Genetics of Man

Human X-Linked genes regionally mapped utilizing X-autosome translocations and somatic cell hybrids.

Gene Localizations and Other Properties of Human Chromosomes

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