Report of the committee on the genetic constitution of chromosomes 13 to 22

Ferguson-Smith, M.A.; Westerveld, A.

doi:10.1159/000130922

Cited by 4 publications

(4 citation statements)

References 1 publication

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“…Chromosomes 25 of Aotus K-VI and 2 of Aotus K-V arc homologous to HSA 19, MML 19, and CCA 8, in that they all share the gene locus coding for GPI Creau-Goldberg et al, 1981;Ferguson-Smith and Westerveld, 1981). However, the remarkable conservation in morphology among HSA 19, MML 19, and CCA 8 is not shared by the two Aotus homologs.…”

Section: Discussionmentioning

confidence: 98%

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Comparative gene mapping of the owl monkey, <i>Aotus, </i>with karyotypes V (2n = 46) and VI (2n = 49, 50)

Ma¹

1983

Cytogenet Genome Res

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Biochemical analysis of cytogenetically defined rodent-owl monkey hybrid cell lines segregating owl monkey chromosomes allowed the assignment of 14 structural gene markers to seven owl monkey karyotype V (K-V, 2n = 46) chromosomes. Comparative study of assigned gene loci to chromosomes of owl monkey karyotype V (K-V, 2n = 46) and karyotype VI (K-VI, 2n = 49/50) provides the first evidence supporting some of the previously proposed mechanics of chromosomal rearrangements responsible for the great karyotypic diversity in owl monkeys. In addition, chromosome homologies deduced from gene loci of Aotus and other primates are compared here to determine the conservation or modification of chromosome morphology in the course of primate evolution. The data demonstrate the usefulness of gene mapping as an alternative to banding patterns for obtaining evidence on chromosome evolution.

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Section: Discussionmentioning

confidence: 98%

“…The gene loci coding for MPI, PKM2, and SORD have been assigned to HSA 15, whereas NP is located on HSA 14 (Ferguson-Smith and Westerveld, 1981). The synteny of NP with PKM2, which has been assigned to chromosome 7 of Pupio papio (PPA) and chromosome 11 of Aotus K-V and K-VI.…”

Section: Discussionmentioning

confidence: 99%

Comparative gene mapping of the owl monkey, <i>Aotus, </i>with karyotypes V (2n = 46) and VI (2n = 49, 50)

Ma¹

1983

Cytogenet Genome Res

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“…To provide further evidence of the identity of the small human chromosome responsible for the expression of the highleucine-transport phenotype, several high-and low-leucinetransport clones, obtained from the segregation procedures, were analyzed for the presence of human chromosome markers. We concentrated these studies on isozyme analyses of the specific markers for human chromosome 20, ADA, and 21, cytoplasmic form of superoxide dismutase (8,23), which were the two small human chromosomes tentatively assigned to the hybrid cell line 158CnpT-1, (Fig. 5, 6).…”

Section: Methodsmentioning

confidence: 99%

Characterization of a Chinese hamster-human hybrid cell line with increased system L amino acid transport activity.

Lobatón¹,

Moreno²,

Oxender³

1984

Mol. Cell. Biol.

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We have studied leucine transport in several Chinese hamster-human hybrid cell lines obtained by fusion of a temperature-sensitive line of Chinese hamster ovary cells, tsO25C1, and normal human leukocytes. A hybrid cell line exhibiting a twofold increase in L-leucine uptake over that in the parental cell line was found. This hybrid cell line, 158CnpT-1, was temperature resistant, whereas the parental Chinese hamster ovary mutant, tsO25C1, contained a temperature-sensitive leucyl-tRNA synthetase mutation. An examination of the different amino acid transport systems in this hybrid cell line revealed a specific increase of system L activity with no significant changes in systems A and ASC. The Vmax for L-leucine uptake exhibited by the hybrid 158CnpT-1 was twice that in the CHO parental mutant, ts025C1. Cytogenetic analysis showed that the hybrid 158CnpT-1 contains four complete human chromosomes (numbers 4, 5, 10, and 21) and three interspecific chromosomal translocations in a total complement of 34 chromosomes. Biochemical and cytogenetic analysis of segregant clones obtained from hybrid 158CnpT-1 showed that the primary temperature resistance and high system L transport phenotypes can be segregated from this hybrid independently. The loss of the primary temperature resistance was associated with the loss of the human chromosome 5, as previously reported by other laboratories, whereas the loss of the high leucine transport phenotype, which is associated with a lesser degree of temperature resistance, was correlated with the loss of human chromosome 20. Amino acid accumulation in mammalian cells is carried out by several distinct transport systems. The major components for neutral amino acid uptake in mammalian cells are systems A, ASC, and L, which were first identified in Ehrlich ascites tumor cells (5, 26). System A is Na+ dependent and serves mainly for the uptake of amino acids with short, polar, or linear side chains. System L is Na+ independent, shows reactivity toward branched-chain and aromatic amino acids, and exhibits rapid exchange between intracellular and extracellular substrates. System ASC is Na+ dependent and shows a strong preference for alanine, serine, and cysteine. Similar systems have been characterized in a number of different cell lines (1,4,9,13,16,27). Recently, transport systems A, ASC, and L have been characterized in Chinese hamster ovary (CHO) cells (2,34). Studies on system L regulation has also been carried out in these cells (24,33,(35)(36)(37) by using a temperature-sensitive leucyl-tRNA synthetase mutant, the CHO tsHl line. When this mutant is incubated at marginally permissive temperatures, a significant increase in leucine transport activity occurs, indicating that the cell responds to leucine limitation resulting from the defective leucyl-tRNA synthetase. Elevated leucine levels in the growth medium, however, allow CHO tsHl cells to grow at nonpermissive temperatures, indicating that increased uptake of leucine is associated with temperature resistance in this mutant. In ad...

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“…1, lanes 3 and 14) showed bands of 10.0, 6.0, 3.1, and 2.65 kb. Each cell hybrid DNA contains mouse bands; however, the human signal segregates, with some hybrids lacking (lanes 5, 7,9,11,19,20), and some containing all three human bands. Co-segregation of the human bands indicated they originated from the same chromosome.…”

Section: Resultsmentioning

confidence: 99%

Regional location of T cell receptor gene Ti alpha on human chromosome 14.

Barker¹,

Royer²,

Ruddle³

et al. 1985

The Journal of Experimental Medicine

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The human T cell receptor is a molecular complex consisting of a clonotypic, disulfide-linked heterodimer, termed Ti, and three noncovalently associated monomorphic T3 components (1). The Ti molecule that presumably serves as the binding site for nominal antigen and the major histocompatibility complex (MHC) is composed of a 49-54 kilodalton (kD) a subunit and a 43 kD/3 subunit (1). In contrast, the 20-25 kD T3 proteins are likely involved in signal transduction after ligand binding to Ti (1). Sequence analysis at both the protein and DNA level indicates that the Ti o~ and/3 subunits are comprised of immunoglobulin-like domains and that each contains variable, joining, and constant (V, J, C) region encoding segments that rearrange during T lineage ontogeny (1).Recent chromosomal mapping studies (2-4) localized the human T cell receptor/3 chain gene (TiB) to human chromosome 7, although its regional position remains unclear. Here, we demonstrate that human chromosome region 14pter>q21 contains the human Tio~ gene. This is of particular cytogenetic interest, since chromosomal inversions and translocations involving this region have recently been observed in human T cell malignancies (5). Furthermore, the apparent lack of genetic linkage between the Tio~ gene and the IgH cluster in humans can now be explained by the great physical distance between these loci on human chromosome 14. Materials and MethodsCell hybrid analysis (6) and blot hybridization experiments (3, 6) followed established methods except that the electro-eluted insert was labeled by the calf thymus primer protocol (7).Isolation of Tic~ REX cDNA Clones. Based on the previously determined amino acid sequence of the Tia REX subunit, we have synthesized an oligonucleotide of 23 bases in length with a complexity of 256 (1). This oligonucleotide pool was used to screen the previously described REX lambda gtl0 cDNA library (1). Positives were isolated and inserts subcloned in pBR322 (paREX) (8). DNA sequence analysis of paREX indicated that it encoded a portion of the V, J, and C regions of the REX human Tia subunit (~400 nucleotides long).

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Report of the committee on the genetic constitution of chromosomes 13 to 22

Cited by 4 publications

References 1 publication

Comparative gene mapping of the owl monkey, <i>Aotus, </i>with karyotypes V (2n = 46) and VI (2n = 49, 50)

Comparative gene mapping of the owl monkey, <i>Aotus, </i>with karyotypes V (2n = 46) and VI (2n = 49, 50)

Characterization of a Chinese hamster-human hybrid cell line with increased system L amino acid transport activity.

Regional location of T cell receptor gene Ti alpha on human chromosome 14.

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