Report of Interaction Between Calf Thymus DNA and Pyrimidine-Annulated Spiro-Dihydrofuran

Abstract

The study of binding interaction between pyrimidine-annulated spiro-dihydrofuran (PSDF) with calf thymus DNA (CTDNA) using UV absorption, fluorescence, circular dichroism (CD), and molecular docking methods. The experimental results revealed that PSDF preferred to bind to the groove of CTDNA with the binding constant (K) of 1.51×10 2 L/mol at 293 K. Based on the signs and magnitudes of the enthalpy change (ΔH = -33.25 kJ/mol) and entropy change (ΔS= -71.58 J/mol/K) in the binding process it can be concluded that the main interaction forces between PSDF and CTDNA in the binding process were van der Waals force and hydrogen bonding interaction. The results of CD experiments revealed that PSDF did not disturb native conformation of CTDNA and the significant binding of PSDF in PSDF-CTDNA complex was observed from the molecular docking results.

One‐potandtwo‐potmethods for chalcone derived pyrimidines synthesis and applications

“…[210] Another quinolinyl pyrimidine series (66) achieved from the reaction of chalcone (63) with guanidine hydrochloride in DMF in the presence of NaH at reflux, achieved 45%-68% yield. [211] One more quinolinepyrimidine series (67) successfully synthesized from (64) and guanidine nitrate in basic media with 78%-84% yield [212] (Scheme 16). Both 65 and 67 exhibited mild to moderate antimicrobial activities that probably increased due to electron-withdrawing groups ( NO 2 , Cl, Br) specifically at phenolic ring.…”

“…[18] In therapeutic industry, pyrimidine derivatives have also been reported to have a broad spectrum of biological properties, that is, anticancer, [19][20][21][22][23][24][25][26][27][28][29][30] antihistamine, [31,32] antinociceptive, [33] antilipase, [34] antifilarial, [35] antiparasitic, [36] antioxidant, [37,38] antituberculosis, [39,40] antihypertensive, [41] antimicrobial, [42][43][44][45][46][47] anticonvulsant, [48,49] antiallergic, [50,51] antipyretic, [52] anti-inflammatory. [53,54] antifungal, [55,56] antiviral, [57][58][59] antibacterial, [60] antimalarial, [61] anti-HIV, [62,63] antiulcer, [64] insecticide, …”

Interaction of Two Flavonoids With Calf Thymus Dna: A Multi - Spectroscopic, Electrochemical and Molecular Modelling Approach

“…Groove binding, however, does not put so much impact on the CD signal in this report. [44][45][46][47] …”