Report of a new <i>meq</i> gene size: The first study on genetic characterisation of Marek’s disease viruses circulating in Iranian commercial layer and backyard chicken

Molouki, Aidin; Ghalyanchilangeroudi, Arash; Abdoshah, Mohammad; Shoushtari, A; Abtin, Alireza; Eshtartabadi, Fatemeh; Akhijahani, Mohsen Mahmoudzadeh; ZiafatiKafi, Zahra; Babaeimarzango, S. S.; Allahyari, Esmaeel; Ahmadzadeh, L.; Mehrabadi, Mohammad Hossein Fallah; Lim, Swee Hua Erin; Rouhani, K.; Hosseini, Hossein; Nair, Venugopal

doi:10.1080/00071668.2021.1963677

Cited by 4 publications

(4 citation statements)

References 39 publications

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“…A 64-aa deletion was observed in the transactivation domain of Kc-c1 VS-Meq compared to Meq. This deletion was similar to that observed in a previously reported Japanese isolate (Sit-c1); however, the size of the deletion was different from that observed in a 99-35 Iranian strain (74-aa deletion) [26], MSB1 and MTB1 cells (92-aa deletion), and T cell lines derived from chickens with MD [29]. The number of PPPP/PXPP sequences in VS-Meq (99-35, and Kc-c1) was lower than that in Meq, and one PPPP sequence was present in the proline-rich regions of VS-Meq (Table 2, Figure 1B).…”

Section: Comparison Of Amino Acid Sequences Of Meqsupporting

confidence: 83%

“…Kc-c1 VS-Meq contained a 64-aa deletion in the transactivation domain of Meq, resulting in a decreased number of PPPP sequences. An MDV strain encoding VS-Meq has been previously detected in unvaccinated backyard chickens in Iran [ 26 ]. Kc-c1 VS-Meq was detected in the MDVs of unvaccinated chickens.…”

Section: Discussionmentioning

confidence: 99%

“…Most MDVs with S-Meq were isolated from unvaccinated chickens or turkeys, and S-Meq contains a 41-amino-acid (aa) deletion in the proline-rich region. In 2022, a very short isoform of Meq (VS-Meq) was reported for the first time in unvaccinated backyard chickens in Iran as a field isolate [ 26 ]. The VS-Meq of the Iranian MDV strains contained one PPPP sequence, whereas the S-Meq contained two or three PPPP sequences.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of a Very Short Meq Protein Isoform in a Marek’s Disease Virus Strain in Japan

Motai,

Murata,

Sato

et al. 2024

Veterinary Sciences

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Marek’s disease virus (MDV) causes malignant lymphoma (Marek’s disease; MD) in chickens. The Meq protein is essential for tumorigenesis since it regulates the expression of host and viral genes. Previously, we reported that the deletion of the short isoform of Meq (S-Meq) decreases the pathogenicity of MDV. Recently, we identified a further short isoform of Meq (very short isoform of Meq, VS-Meq) in chickens with MD in Japan. A 64-amino-acid deletion was confirmed at the C-terminus of VS-Meq. We measured the transcriptional regulation by VS-Meq in three gene promoters to investigate the effect of VS-Meq on protein function. Wild-type VS-Meq decreased the transrepression of the pp38 promoter but did not alter the transactivation activity of the Meq and Bcl-2 promoters. The deletion in VS-Meq did not affect the activity of the pp38 promoter but enhanced the transactivation activities of the Meq and Bcl-2 promoters. Collectively, the deletion of VS-Meq potentially enhanced the activity of the Meq promoter, while other amino acid sequences in wild-type VS-Meq seemed to affect the weak transrepression of the pp38 promoter. Further investigation is required to clarify the effects of these changes on pathogenicity.

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Section: Comparison Of Amino Acid Sequences Of Meqsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Characterization of a Very Short Meq Protein Isoform in a Marek’s Disease Virus Strain in Japan

Motai,

Murata,

Sato

et al. 2024

Veterinary Sciences

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“…MDV strains encoding L-Meq were originally found in field strains from the 1960s and 1970s with low virulence in the US and an attenuated vaccine strain, CVI988 [19], whereas MDV strains with S-Meq have been reported in the field since the 2010s. Moreover, a very short isoform of Meq, which encodes one copy of PRR in the transactivation domain, was reported in a virulent MDV strain circulating in Iran [45]. Although the pathogenicity of such field strains having the S-Meq is uncertain, MDV strains with short isoforms of Meq have emerged more recently than MDV strains with L-Meq and Meq along the timeline.…”

Section: Discussionmentioning

confidence: 99%

Effect of Insertion and Deletion in the Meq Protein Encoded by Highly Oncogenic Marek’s Disease Virus on Transactivation Activity and Virulence

Sato

Shiro

Yang

et al. 2022

Viruses

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Marek’s disease virus (MDV) causes malignant lymphoma in chickens (Marek’s disease, MD). Although MD is currently controlled by vaccination, MDV strains have continuously increased in virulence over the recent decades. Polymorphisms in Meq, an MDV-encoded oncoprotein that serves as a transcription factor, have been associated with the enhanced virulence of the virus. In addition, insertions and deletions in Meq have been observed in MDV strains of higher virulence, but their contribution to said virulence remains elusive. In this study, we investigated the contribution of an insertion (L-Meq) and a deletion in the Meq gene (S-Meq) to its functions and MDV pathogenicity. Reporter assays revealed that both insertion and deletion enhanced the transactivation potential of Meq. Additionally, we generated RB-1B-based recombinant MDVs (rMDVs) encoding each Meq isoform and analyzed their pathogenic potential. rMDV encoding L-Meq indueced the highest mortality and tumor incidence in infected animals, whereas the rMDV encoding S-Meq exhibited the lowest pathogenicity. Thus, insertion enhanced the transactivation activity of Meq and MDV pathogenicity, whereas deletion reduced pathogenicity despite having increased transactivation activity. These data suggest that other functions of Meq affect MDV virulence. These data improve our understanding of the mechanisms underlying the evolution of MDV virulence.

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Phylogenetic analyses on Marek’s disease virus circulating in Iranian backyard and commercial poultry indicate viruses of different origin

et al. 2022

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Report of a new meq gene size: The first study on genetic characterisation of Marek’s disease viruses circulating in Iranian commercial layer and backyard chicken

Cited by 4 publications

References 39 publications

Characterization of a Very Short Meq Protein Isoform in a Marek’s Disease Virus Strain in Japan

Characterization of a Very Short Meq Protein Isoform in a Marek’s Disease Virus Strain in Japan

Effect of Insertion and Deletion in the Meq Protein Encoded by Highly Oncogenic Marek’s Disease Virus on Transactivation Activity and Virulence

Phylogenetic analyses on Marek’s disease virus circulating in Iranian backyard and commercial poultry indicate viruses of different origin

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