Repopulation of the immunosuppressed retrorsine‐treated infant rat liver with human hepatocytes

Abstract

Xenogeneic hepatocytes were able to engraft rat liver and grow well therein for at least 3 weeks post-transplantation in rats when immunosuppression was combined appropriately with liver injury at comparable levels to the well-characterized humanized chimeric mouse model.

Transplantation of a human iPSC-derived hepatocyte sheet increases survival in mice with acute liver failure

“…Experimental investigations using rodent models are expected to contribute technical improvements to the clinical methods of hepatocyte transplantation. In previous reports, human hepatocytes and human iPS-HLCs were transplanted into the rodents with liver injury by either intrasplenic or portal venous infusion [6][7][8]. These hepatocyte transplantation methods have some disadvantages, including difficulties with the regulation of engraftment efficiency and the potential for engraftment in other organs, since the transplanted hepatocytes are distributed into other organs as well as the liver following delivery through blood circulation [9,10].…”

The Development of Humanized Liver With Rag1 Knockout Rats

“…Humanized liver animals are useful for testing of drug metabolism and toxicity in pre-clinical studies. It is reported in mouse, which the liver is repopulated more than 90% with human-hepatocytes (1), but in rat the target is far from being reached (2)(3)(4). Since the rat has historically been the preferred animal model for biomedical research, with physiology closer to humans and with larger sample volumes, to develop a humanized liver model with rat has an increased need.…”

Liver cell therapy: is this the end of the beginning?

“…LPCs, PHH, and human iPS-HLCs have been transplanted into the rodents with liver injury, by intrasplenic or portal venous infusion [ 166 , 167 ]. A suspension of BM-derived HLCs (0.5 × 10 6 –2 × 10 8 ) has been also injected intravenously, intrasplenically or intrahepatically with ultrasonographic guidance [ 124 ].…”