Repopulation of an auricular cartilage scaffold, AuriScaff, perforated with an enzyme combination

Nürnberger, Sylvia; Schneider, C.; Osch, Gerjo van; Keibl, Claudia; Rieder, Bernhard; Monforte, Xavier; Teuschl, Andreas; Mühleder, Severin; Holnthoner, Wolfgang; Schädl, Barbara; Gahleitner, Claudia; Redl, Heinz; Wolbank, Susanne

doi:10.1016/j.actbio.2018.12.035

Cited by 29 publications

(27 citation statements)

References 73 publications

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“…Conversely, all the reviewed MSC cell lines immortalized with SV40LT maintained their adipogenic potential. The adipose-tissue-derived ASC/TERT1 cell line showed an increase in adipogenic potential after immortalization with hTERT [22]. Once more, iSUPS differentiation potential towards the adipogenic lineage was increased when SV40LT was removed [50].…”

Section: Adipogenic Potentialmentioning

confidence: 99%

“…tested in pellet culture [15] Positive for OROS and PPARγ upregulation [15] ASC/TERT1 Positive for VKS and ALP activity [84] Positive for ABS, trichrome staining and ColII immunostaining in 3D scaffolds; reduced cartilage quality in comparison with chondrocytes [22] Positive for OROS and PPARγ upregulation; adipogenic potential increased compared with primary cells [84] hASCs-T Positive for APS; reduced osteogenic potential in comparison with primary cells [85] Not tested Tested by OROS, but almost no lipid droplets detected [85] GB/hTERT MSCs Positive for ARS; reduced compared with primary cells [86] Not tested Positive for OROS [86] SDP11 Positive for BMP-2 and ALP upregulation [88] Not tested Positive for OROS but not shown [88] Pelt cells…”

Section: Y201mentioning

confidence: 99%

“…In many cases, the chondrogenic potential of iMSCs has been scarcely analyzed by one single histochemical staining, either alcian blue staining (ABS) or toluidine blue staining (TBS) [14,54,55,71,91,101]. Although chondrogenic transcription factor Sox9 and type II collagen upregulation have been detected in chondrogenic-induced iMSCs, these cells showed the same proneness to hypertrophy as primary MSCs, with type X collagen expression [15,35] and low-quality cartilage production [22] with scarce exceptions [41,94], even when three-dimensional culture was performed [15,22,35]. This low-quality cartilage generation is relatively common among MSCs.…”

Section: Chondrogenic Potentialmentioning

confidence: 99%

“…Nowadays, a high number of MSC lines that display specific characteristics and differentiation capabilities have been generated and are valuable tools as part of models of disease and tissue repairing strategies. Different MSC lines have been employed for testing [17,[20][21][22][23][24][25] or producing [26,27] engineered scaffolds for skeletal applications, and for both investigating the MSC differentiation process [28][29][30][31][32][33][34] and finding new ways to improve it [35][36][37][38][39]. Additionally, these cell lines have also been used for analyzing functional makers [19,40] or even for exploring their roles in different diseases, such as osteoarthritis [41,42].…”

Section: Introductionmentioning

confidence: 99%

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Usefulness of Mesenchymal Cell Lines for Bone and Cartilage Regeneration Research

Piñeiro-Ramil

Sanjurjo‐Rodríguez

Castro-Viñuelas

et al. 2019

IJMS

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The unavailability of sufficient numbers of human primary cells is a major roadblock for in vitro repair of bone and/or cartilage, and for performing disease modelling experiments. Immortalized mesenchymal stromal cells (iMSCs) may be employed as a research tool for avoiding these problems. The purpose of this review was to revise the available literature on the characteristics of the iMSC lines, paying special attention to the maintenance of the phenotype of the primary cells from which they were derived, and whether they are effectively useful for in vitro disease modeling and cell therapy purposes. This review was performed by searching on Web of Science, Scopus, and PubMed databases from 1 January 2015 to 30 September 2019. The keywords used were ALL = (mesenchymal AND ("cell line" OR immortal*) AND (cartilage OR chondrogenesis OR bone OR osteogenesis) AND human). Only original research studies in which a human iMSC line was employed for osteogenesis or chondrogenesis experiments were included. After describing the success of the immortalization protocol, we focused on the iMSCs maintenance of the parental phenotype and multipotency. According to the literature revised, it seems that the maintenance of these characteristics is not guaranteed by immortalization, and that careful selection and validation of clones with particular characteristics is necessary for taking advantage of the full potential of iMSC to be employed in bone and cartilage-related research.

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Section: Adipogenic Potentialmentioning

confidence: 99%

Section: Y201mentioning

confidence: 99%

Section: Chondrogenic Potentialmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Usefulness of Mesenchymal Cell Lines for Bone and Cartilage Regeneration Research

Piñeiro-Ramil

Sanjurjo‐Rodríguez

Castro-Viñuelas

et al. 2019

IJMS

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“…To examine the performance of CartiScaff in vivo in a cartilage defect environment, reseeded scaffolds were implanted into experimental cartilage defects in osteochondral plugs and kept subcutaneously in nude mice [45] .…”

Section: Methodsmentioning

confidence: 99%

Repopulation of decellularised articular cartilage by laser-based matrix engraving

et al. 2021

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Background In spite of advances in the treatment of cartilage defects using cell and scaffold-based therapeutic strategies, the long-term outcome is still not satisfying since clinical scores decline years after treatment. Scaffold materials currently used in clinical settings have shown limitations in providing suitable biomechanical properties and an authentic and protective environment for regenerative cells. To tackle this problem, we developed a scaffold material based on decellularised human articular cartilage. Methods Human articular cartilage matrix was engraved using a CO 2 laser and treated for decellularisation and glycosaminoglycan removal. Characterisation of the resulting scaffold was performed via mechanical testing, DNA and GAG quantification and in vitro cultivation with adipose-derived stromal cells (ASC). Cell vitality, adhesion and chondrogenic differentiation were assessed. An ectopic, unloaded mouse model was used for the assessment of the in vivo performance of the scaffold in combination with ASC and human as well as bovine chondrocytes. The novel scaffold was compared to a commercial collagen type I/III scaffold. Findings Crossed line engravings of the matrix allowed for a most regular and ubiquitous distribution of cells and chemical as well as enzymatic matrix treatment was performed to increase cell adhesion. The biomechanical characteristics of this novel scaffold that we term CartiScaff were found to be superior to those of commercially available materials. Neo-tissue was integrated excellently into the scaffold matrix and new collagen fibres were guided by the laser incisions towards a vertical alignment, a typical feature of native cartilage important for nutrition and biomechanics. In an ectopic, unloaded in vivo model, chondrocytes and mesenchymal stromal cells differentiated within the incisions despite the lack of growth factors and load, indicating a strong chondrogenic microenvironment within the scaffold incisions. Cells, most noticeably bone marrow-derived cells, were able to repopulate the empty chondrocyte lacunae inside the scaffold matrix. Interpretation Due to the better load-bearing, its chondrogenic effect and the ability to guide matrix-deposition, CartiScaff is a promising biomaterial to accelerate rehabilitation and to improve long term clinical success of cartilage defect treatment. Funding Austrian Research Promotion Agency FFG (“CartiScaff” #842455), Lorenz Böhler Fonds (16/13), City of Vienna Competence Team Project Signaltissue (MA23, #18-08)

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Nature‐Inspired Strategies for the Treatment of Osteoarthritis

Wu,

Zheng,

et al. 2023

Adv Funct Materials

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Articular cartilage is devoid of nerves and blood vessels, and its nutrients must be obtained from the joint fluid; therefore, its ability to repair itself is limited. Manufactured materials such as artificial cartilage or synthetic materials are typically used in traditional approaches for knee cartilage repair. However, durability, postimplant rejection, and tissue incompatibility are the problems associated with these materials. In recent decades, tissue engineering and regenerative medicine have focused on the development of functional substitutes, particularly those based on naturally inspired biopolymers. This review focuses on sustainably produced biopolymers based on materials derived from natural sources. Furthermore, these materials have many advantages, including low antigenicity, biocompatibility, and degradability. Of course, there are also many challenges associated with natural materials, such as the lack of clinical studies and long‐term follow‐up data, unstable mechanical properties of the materials, and high demands placed on preparation and molding techniques. In this review, an overview of natural and nature‐inspired polymers that are the subject of research to date, as well as their structural designs and product performances is provided. This review provides scientific guidance for enhancing the development of naturally inspired materials for treating cartilage injuries.

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Repopulation of an auricular cartilage scaffold, AuriScaff, perforated with an enzyme combination

Cited by 29 publications

References 73 publications

Usefulness of Mesenchymal Cell Lines for Bone and Cartilage Regeneration Research

Usefulness of Mesenchymal Cell Lines for Bone and Cartilage Regeneration Research

Repopulation of decellularised articular cartilage by laser-based matrix engraving

Nature‐Inspired Strategies for the Treatment of Osteoarthritis

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