Repopulation of a human alveolar matrix by adult rat type II pneumocytes in vitro

Lwebuga‐Mukasa, Jamson S.; Ingbar, David H.; Madri, Joseph A.

doi:10.1016/0014-4827(86)90347-2

Cited by 109 publications

(68 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The PBS was removed and replaced with 20 ml of complete medium (DMEM, 10% FBS, and 300 IU/ml penicillin, 300 IU/ml streptomycin, and 75 μg/ml amphotericin B) and shaken overnight at room temperature. This decellularization procedure is similar to a previously described procedure (50). Tissue slices were incubated (37°C, 5% CO2) for 1 hour prior to seeding with cells.…”

Section: Discussionmentioning

confidence: 99%

Fibrotic extracellular matrix activates a profibrotic positive feedback loop

Parker¹,

Rossi²,

Peterson³

et al. 2014

J. Clin. Invest.

466

416

View full text Add to dashboard Cite

Pathological remodeling of the extracellular matrix (ECM) by fibroblasts leads to organ failure. Development of idiopathic pulmonary fibrosis (IPF) is characterized by a progressive fibrotic scarring in the lung that ultimately leads to asphyxiation; however, the cascade of events that promote IPF are not well defined. Here, we examined how the interplay between the ECM and fibroblasts affects both the transcriptome and translatome by culturing primary fibroblasts generated from IPF patient lung tissue or nonfibrotic lung tissue on decellularized lung ECM from either IPF or control patients. Surprisingly, the origin of the ECM had a greater impact on gene expression than did cell origin, and differences in translational control were more prominent than alterations in transcriptional regulation. Strikingly, genes that were translationally activated by IPF-derived ECM were enriched for those encoding ECM proteins detected in IPF tissue. We determined that genes encoding IPF-associated ECM proteins are targets for miR-29, which was downregulated in fibroblasts grown on IPF-derived ECM, and baseline expression of ECM targets could be restored by overexpression of miR-29. Our data support a model in which fibroblasts are activated to pathologically remodel the ECM in IPF via a positive feedback loop between fibroblasts and aberrant ECM. Interrupting this loop may be a strategy for IPF treatment.

show abstract

Section: Discussionmentioning

confidence: 99%

Fibrotic extracellular matrix activates a profibrotic positive feedback loop

Parker¹,

Rossi²,

Peterson³

et al. 2014

J. Clin. Invest.

466

416

View full text Add to dashboard Cite

show abstract

“…The concept of decellularization was introduced in the late 1980's in biopsy material where rat alveolar matrix was prepared to study the role of basement membrane on alveolar epithelial cells. 9 The first successful whole organ decellularization was achieved in 2008 by the group of Doris Taylor demonstrating a 3-dimentional structure of cadaveric hearts, which were repopulated with epithelial and cardiac cells. 10 Momentarily, intricate decellularization protocols have been developed for nearly all organs.…”

Section: Matricesmentioning

confidence: 99%

Tissue Engineering of the Trachea: What is the Hold-up?

Siddiqi¹,

Wit²,

Timman³

et al. 2017

MOJCSR

View full text Add to dashboard Cite

Tissue-engineering of the trachea is called for based on the clinical consequences of airway pathology. Recent years have witnessed various reports from the tissueengineering field including a few cases of clinical patients receiving an engineered airway. Multiple fields have accelerated in parallel with the concept of tissue decellularization, isolation of stem cells and possible insights in the biology of different stem cells. Yet, a clinical trial using a tissue-engineered trachea is yet to be initiated. What is the hold-up? Are the hurdles predominantly in the matrix field or cellular regeneration field or both? This systematic review is an attempt to evoke question on what the hold-up might be in the journey of successful regeneration of a functional airway.

show abstract

“…Given the important functional requirement for a reseeded lung to oxygenate and ventilate, there has been appropriate focus on the alveolar epithelium, which consists of two major types: squamous alveolar epithelial type I (ATI) cells, which occupy a majority of the alveolar surface area in the native lung, and surfactant-producing cuboidal type II (ATII) cells, which occupy a small area but constitute Ͼ65% of the epithelial cell population (105). Transformation of ATII cells to ATI cells upon reseeding onto an acellular human alveolar matrix was demonstrated a number of years ago (105). Fetal cells (typically derived from the native organ of interest, and in nonhuman species) have been reported in several studies for tissue bioengineering and seeding of scaffolds (156, 174, 182).…”

Section: Recellularization In Lung Bioengineeringmentioning

confidence: 99%