“…SECM, which uses a microelectrode for detecting localized electroactive chemical concentration as faradaic current with noninvasive, is an effective tool for estimating oxygen consumption of a single living cell and detecting enzyme activity. [1][2][3][4][5] To evaluate differentiation state of ES cells, we measured intracellular alkaline phosphatase (ALP) expression level. ALP is known as a differentiationmarker and the expression level of ALP decreases during differentiation process.…”