Reply: Supplementation with Extracellular Vesicles Derived from Adipose-Derived Stem Cells Increases Fat Graft Survival and Browning in Mice: A Cell-Free Approach to Construct Beige Fat from White Fat Grafting
Abstract:of inguinal fat and fat injection. The authors injected fat particles with a 19-gauge needle. We usually use a 16-gauge rather than a 19-gauge needle based on the following considerations: on the one hand, the fine needle produces high shear force, which impairs graft viability. 2 On the other hand, the fat particles need to be cut smaller to pass through the fine needle, leading to long extracorporeal ischemia time.
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