Replication of the bacteriocinogenic plasmid Clo DF13: Action of the plasmid protein cloacin DF13 on Clo DF13 DNA

Veltkamp, E.; Pols, Kees; Ee, Jan H. van; Nijkamp, H. J. J.

doi:10.1016/0022-2836(76)90301-6

Cited by 10 publications

(3 citation statements)

References 47 publications

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“…However, 2 partial fragments could be observed with molecular weights of about 0.70 and 0.53 x 10 daltons (data not shown), indicating that EcoRI-5 is flanked by two Hind II sites at a distance of about 0.11 and 0.28 x 10 daltons, respectively. The piece of 0.11 x 10 daltons can only be placed at the left of EcoRI-5 in the double digestion map, since together with Hind II-4 (a,b) and Hind II (7) 022 (8) 037(6) Figure 4. A physical map of the cleavage products generated by redigestion of the Hind II fragments with EcoRI and double digestion of yeast rDNA with a combination of EcoRI and Hind II.…”

Section: Complete Digestion Of Yeast Rdna With Ecorimentioning

confidence: 99%

Topographical analysis of yeast ribosomal DNA by cleavage with restriction endonucleases. II. The physical map of EcoRI fragments

Meyerink

Retèl

1976

Nucleic Acids Research

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Yeast ribosomal DNA (rDNA) was digested with the restriction endonuclease EcoRI. Eight distinct fragments were obtained with a molecular weight of 4.35 (1), 1.75 (2), 1.45 (3), 1.07 (4), 0.42 (5), 0.37 (6), 0.26 (7) and 0.22 x 10(6) (8) daltons, respectively. Except for fragment 1 with a molecular weight of 4.35 x 10(6) daltons, all fragments are derived from the multiple ribosomal transcription units. The 'spacer' sequences, on the other hand, gave rise to digestion products which are very heterogeneous in size. By analysis of the partial digestion products which are very heterogeneous in size. By analysis of the partial digestion products, together with the data obtained by digestion with a combination of two restriction enzymes (EcoRI and Hind II or Hind III) and redigestion of the Hind II-and Hind III-fragments with EcoRI, the physical map of the EcoRI cleavage sites in the ribosomal transcription unit could be established.

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Section: Complete Digestion Of Yeast Rdna With Ecorimentioning

confidence: 99%

Topographical analysis of yeast ribosomal DNA by cleavage with restriction endonucleases. II. The physical map of EcoRI fragments

Meyerink

Retèl

1976

Nucleic Acids Research

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“…Even Instances of in vivo crypticity of bacterial gene products have resulted previously from the absence of genes that encode accessory proteins required for phenotypic expression of the primary product (33) or from complex formation of the primary product with a second protein (34). A recent report indicates that the Clo DF13 plasmid enclodes a site-specific endonuclease that cleaves Clo DF13 DNA in vitro, but that the Clo DF13 immunity product forms a complex with the endonuclease to neutralize its activity in vvo (34). Other observations indicate that the site-specific endonuclease made by Bacillus subtilis strain Marburg 168 can cleave in vitro the DNA of a bacteriophage grown on the same strain (35).…”

Section: Methodsmentioning

confidence: 99%

Phenotypically cryptic EcoRI endonuclease activity specified by the ColE1 plasmid.

Miller¹,

Cohen²

1978

Proc. Natl. Acad. Sci. U.S.A.

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(1), is encoded by genes located on a bacterial plasmid that has been designated pMB1 (2); a second site-specific enzyme, which methylates the adenosine at the center of the nucleotide sequence comprising the EcoRI endonuclease cleavage site (3) thereby rendering the site insusceptible to cleavage, is also specified by the pMB1 plasmid. It has been reported that a large segment of the pMB1 plasmid is homologous with another widely studied bacterial plasmid, ColEl (2). Since ColEl DNA is cleaved in vitro by the EcoRI endonuclease (4), and because bacterial cells carrying ColEl are not known to be associated with restriction activity, earlier investigators concluded that the EcoRI restriction and modification genes are located on a 1.95-kilobase segment of DNA that is present in pMB1 but not within ColEl (2). During in vitro studies of possible site-specific DNA cleavage associated with the Tn3 transposon, we observed that bacterial cells carrying the ColEl plasmid itself produce an endonuclease having EcoRI restriction specificity. However ColEl-containing cells do not express EcoRI restriction or modification functions in vivo and plasmid or phage DNA isolated from such cells is cleaved in vitro by the EcoRI endonuclease. The present report describes these findings.MATERIALS AND METHODS Bacterial Strains and Plasmids. E. coli strains RY13, C600, and HB101 (pMB4) were obtained from H. W. Boyer (University of California, San Francisco). E. coli strain 1200 [endo I-, RNase-, rK+mK+ (5)] was provided by M. Pearson. The ColEl-K30 plasmid was obtained from D. Helinski (6), and the Tn3 transposon (7) was introduced onto this plasmid fromThe costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U. S. C. §1734 solely to indicate this fact. 1265 pSC204 by the selected translocation procedure (8). The pMB3 plasmid and the Ri drd-19 plasmid were isolated from E. coli RY13 and purified by a cycle of transformation (9). The pMB3 and pMB4 (a spontaneous deletion mutant of pMB3) plasmids, which specify the EcoRI restriction and modification edzymes, are derived from the clinical isolate pMB1 (2). pCM100 is a spontaneously occurring mutant (isolated in our laboratory) of pMB3 that has lost the ability to restrict or modify DNA in vvo. Bacteriophages Xmr and XCI83 (10) and plasmid pSC355 (11) were used for detection of phenotypically expressed restriction and modification functions. Purified X DNA was isolated (12) from Xplac MS505S7 phage (13). Isolation of Enzyme. Bacterial cells used for isolation of endonuclease and methylase were grown in L broth (14) containing appropriate antibiotics, at concentrations of 25 Ag/ml for ampicillin, and 10,gg/ml for kanamycin, chloramphenicol, tetracycline, and streptomycin. Bacterial cultures of 500 ml or less were grown at 370 in shaking water baths to ODW0 = 1.0 and chilled by transfer of flasks to an ice water bath. Cells were collected by centrifugation, suspended (fin...

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“…The enzymatic similarity between these previously considered disparate bacteriocin activities has recently been augmented by the demonstration of endonucleolytic cleavage of the Clo DF13 plasmid by cloacin DF13 (24).…”

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confidence: 99%

Mode of Action of Morganocin 174

Williams

Krizsanovich-Williams

1977

Antimicrob Agents Chemother

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Morganocin 174-induced lethality was characterized by one-hit kinetics. Although it induced simultaneous inhibition of deoxyribonucleic acid, ribonucleic acid, and protein syntheses, the most striking effect of morganocin was a rapid reduction of intracellular adenosine 5'-triphosphate to less than 10% of the initial values within 2 min of addition. Accumulation of thymidine, uridine, glutamine, and proline was also inhibited. A gradual efflux of K+ was observed, but the lethal effects of morganocin 174 could not be prevented by maintaining a high K+ or Mg2+ concentration. The Morl74 plasmid codes for an immunity substance that protects cells against the effect of morganocin 174.

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Replication of the bacteriocinogenic plasmid Clo DF13: Action of the plasmid protein cloacin DF13 on Clo DF13 DNA

Cited by 10 publications

References 47 publications

Topographical analysis of yeast ribosomal DNA by cleavage with restriction endonucleases. II. The physical map of EcoRI fragments

Topographical analysis of yeast ribosomal DNA by cleavage with restriction endonucleases. II. The physical map of EcoRI fragments

Phenotypically cryptic EcoRI endonuclease activity specified by the ColE1 plasmid.

Mode of Action of Morganocin 174

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