Replication of Reovirus

Biochemistry and Immunology of Infectious Bursal Disease Virus

“…Initial steps of viral replication ofdsRNA viruses have been extensively studied with reovirus (Shatkin, 1969) which utilizes a virion-associated RNA polymerase for transcription of one strand of the viral RNA. Similar results have been published with other dsRNA viruses such as bluetongue virus (Martin & Zweerink, 1972;Verwoerd et al, 1972) and cytoplasmic polyhedrosis virus (Lewandowski et al, 1969).…”

Viral Diseases of the Digestive System

“…Currently, knowledge of reovirus replication on biochemical and biophysical planes is as extensive as that available for any other virus and is, for the most part, outside the scope of this chapter. Interested readers are therefore referred to reviews by Shatkin (1969) and Joklik (1974) for extended literature coverage and detailed dis cussion and to Andrewes et al (1978) for a condensed over view.…”

Viruses with segmented ribonucleic acid genomes: multiplication of influenza versus reovirus.

“…Reovirus genome RNA. Double-stranded viral RNA synthesis can be detected in reovirus type 3-infected mouse L cells at about the same time as the increase in viral mRNA formation (48, 134,135). Genome RNA comprises about onefourth of the total virus-directed RNA synthesized during replication.…”

“…These were later supported by the finding that RNA extracted from influenza and other myxoviruses consists of a mixture of RNA fragments of molecular weight lower than that of the total viral RNA estimated chemically (3, 13, 38, 40a, 101, 109). It is now known that the double-stranded RNA genomes of reoviruses and similar animal and plant viruses are made up of distinct segments (47,48,70,134,135,156). Recent studies of a variety of singlestranded RNA viruses including paramyxoviruses (Newcastle disease virus, NDV; reference 75), rhabdoviruses (vesicular stomatitis virus, VSV; references 67, 97,104,121), arboviruses (Sindbis virus; reference 36), and the RNA tumor viruses (4, 40) have shown that viral RNA genomes, or their complement in infected cells, are often found as subunits.…”