1972
DOI: 10.1073/pnas.69.3.737
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Replication of Mitochondrial DNA. Circular Replicative Intermediates in Mouse L Cells

Abstract: The frequency, composition, and structure of circular replicating forms of mitochondrial DNA, including two new forms described here, suggest a scheme for the mode of replication of this DNA.The closed-circular mitochondrial DNA (mtDNA) isolated from exponentially growing mouse L cells contains a substantial fraction of displacement-loop molecules (1). These molecules, formed by displacement synthesis in the first discrete stage of DNA replication, contain a short progeny strand hydrogen-bonded at a unique sit… Show more

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Cited by 187 publications
(134 citation statements)
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“…The most widely accepted "orthodox" hypothesis explaining the mode of mtDNA replication is the so called strand displacement model (Robberson et al, 1972;Goddard and Wolstenholme, 1980;Clayton, 1982). We can give here only a brief outline and refer to the reviews by Shadel and Clayton (1997) and Taanman (1999) for much more detailed description of the replication process.…”
Section: Model(s) Of Replicationmentioning
confidence: 99%
“…The most widely accepted "orthodox" hypothesis explaining the mode of mtDNA replication is the so called strand displacement model (Robberson et al, 1972;Goddard and Wolstenholme, 1980;Clayton, 1982). We can give here only a brief outline and refer to the reviews by Shadel and Clayton (1997) and Taanman (1999) for much more detailed description of the replication process.…”
Section: Model(s) Of Replicationmentioning
confidence: 99%
“…Thus, the most reasonable explanation for all these data is that mtDNA molecules that contain pyrimidine dimers are not repaired but replicate until a dimer is encountered on a parental strand and then replication ceases. If this occurs early in replication [before synthesis of 0.6 genome lengths (8)], the molecule will be arrested as an expanded D-loop molecule. If this occurs late in replication, then the daughter molecules can separate and are isolated as gapped circular molecules that cannot complete duplex synthesis and subsequent closure.…”
Section: Methodsmentioning
confidence: 99%
“…We were therefore interested in determining whether or not mammalian cells contain a repair mechanism(s) for removal of ultraviolet light-induced pyrimidine dimers in mitochondrial DNA (mtDNA). In addition, recent work has established that these DNA molecules replicate in situ and the mechanism of replication has been described (8)(9)(10)(11). If mammalian mitochondria could be shown to carry out repair of damaged DNA, it is possible that some of the previously described presumptive replicating forms of mtDNA might in part be repair intermediates.…”
mentioning
confidence: 99%
“…DNA synthesis from O H is unidirectional and proceeds to displace the parental heavy strand (H-strand). After leading-strand synthesis has reached two-thirds of the genome, it activates O L and lagging-strand DNA synthesis then initiates in the opposite direction (2)(3)(4). Recently, analysis of mtDNA replication intermediates by atomic force microscopy revealed that the strand-displacement model is an oversimplification and that additional initiation sites for lagging-strand mtDNA synthesis exist on the light strand (L-strand) (5).…”
mentioning
confidence: 99%