Replication of DNA Satellites and Their Role in Viral Pathogenesis

Sattar, Muhammad Naeem; Iqbal, Zafar; Hameed, Amir

doi:10.1007/978-3-030-18248-9_9

Cited by 2 publications

(3 citation statements)

References 100 publications

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“…Betasatellites genome has no specific sequence homology with the begomoviruses except nona-nucleotide sequences (TAATATT/AC), which resembles geminivirus-like nona-nucleotide sequences ( Hanley-Bowdoin et al, 2000 ). Betasatellites are very flexible in their trans -replication by a multiple ranges of begomoviruses or even other geminiviruses ( Kharazmi et al, 2012 , Sattar et al, 2019 ). The Rep protein of most of the helper begomoviruses helps in the replication of multiple betasatellites even though these are the non-cognate to a begomovirus (lacking the associated begomovirus iteron sequences).…”

Section: Discussionmentioning

confidence: 99%

Natural occurrence of mesta yellow vein mosaic virus and DNA-satellites in ornamental sunflower (Helianthus spp.) in Pakistan

Sattar

Iqbal

Ali

et al. 2021

Saudi Journal of Biological Sciences

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Weeds and ornamental plants serve as a reservoir for geminiviruses and contribute to their dissemination, genome recombination and/or satellite capture. Ornamental sunflower ( Helianthus spp.) plants exhibiting mild leaf curl symptoms were subjected to begomovirus and DNA-satellites isolation. The full-length genome of the isolated begomovirus clone (Od1-A) showed 96.8% nucleotide (nt) sequence identity with mesta yellow vein mosaic virus (MeYVMV; accession no. FR772081) whereas, alphasatellite (Od1-a) and betasatellite (Od1-b) clones showed their highest nt sequence identities at 97.4% and 98.2% with ageratum enation alphasatellite (AEA; accession no. FR772085) and papaya leaf curl betasatellite (PaLCuB; accession. no. LN878112), respectively. The evolutionary relationships, average evolutionary divergence and the recombination events were also inferred. The MeYVMV exhibited 9.5% average evolutionary divergence and its CP and Rep had 9.3% and 12.2%, concomitantly; the alphasatellite and the betasatellite had 8.3% and 5.2%, respectively. The nt substitution rates (site -1 year −1 ) were found to be 6.983 × 10 -04 and 5.702 × 10 -05 in the CP and Rep of MeYVMV, respectively. The dN/dS ratio and the Tajima D value of MeYVMV CP demonstrated its possible role in host switching. The absolute quantification of the begomovirus demonstrated that mild symptoms might have a correlation with low virus titer. This is the first identification of MeYVMV and associated DNA-satellites from ornamental sunflower in Pakistan. The role of sequence divergence, recombination and importance of MeYVMV along with DNA-satellites in extending its host range is discussed.

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Section: Discussionmentioning

confidence: 99%

Natural occurrence of mesta yellow vein mosaic virus and DNA-satellites in ornamental sunflower (Helianthus spp.) in Pakistan

Sattar

Iqbal

Ali

et al. 2021

Saudi Journal of Biological Sciences

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“…deltasatellite has been identified. These satellite molecules carry circular and single-stranded DNA genome with almost 1.4 kb genome size [13,14] while the genome size of deltasatellite isalmost 700 bp [15]. Betasatellites have expressed their role in symptoms modulation [16] and alphasatellite has been reported to interrupt the replication process of betasatellites but it exerts no effect on replication of begomovirus while there is clear evidence of alphasatllites' role in pathogenicity [17].…”

Section: Fig 1 Simplified Mechanism Of Replication Of Begomovirus Dnamentioning

confidence: 99%

“…Betasatellites have expressed their role in symptoms modulation [16] and alphasatellite has been reported to interrupt the replication process of betasatellites but it exerts no effect on replication of begomovirus while there is clear evidence of alphasatllites' role in pathogenicity [17]. Although, the precise role of deltasatellite is not clear yet [15] but it has been reported that deltasatellite reduces the accumulation of begomovirus DNA and also contributes in suppressing symptoms of infection caused by helper viruses [14] So, deltasatellite is not required by begomoviruses for disease occurrence [18].…”

Section: Fig 1 Simplified Mechanism Of Replication Of Begomovirus Dnamentioning

confidence: 99%

Identifying Genetic Diversity of Cotton Leaf Curl Virus in Commercial Cotton (Gossypium hirsutum L.)

Hussain

Rizwi

Naqqash

et al. 2021

AJBGMB

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Plant viruses have become a major issue to the crop production around the world. These viruses have become an unavoidable limiting factor by causing rigorous crop deficit for those major crops which are economically important. Among these viruses, begomoviruses belonging to family Geminiviridae, have brought the major devastation to both monocots and dicots in tropical and sub-tropical regions. These begomoviruses are transmitted through white fly and cause diseases like mosaic, yellow mosaic, yellow leaf curling, and yellow vein etc. The objective of this study is to evaluate the association between the genetic diversity of begomoviruses and phenotypic data to identify the ideal cotton genotype for breeding. In this study, 30 leaf samples with CLCuD symptoms were analyzed using rolling circular amplification and PCR. Obtained results expressed the presence of begomovirus with its associated satellites (i.e. alpha and betasatellites) in six varieties, association of begomovirus and betasatellites in four varieties and combination of begomovirus with alphasatellite in six varieties. Severe disease symptoms were exhibited with these combinations in selected varieties but betasatellite-begomovirus complex demonstrated a deadly impact. Few varieties expressed the resistance against the begomoviruses, so these varieties can further be manipulated for breeding.

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Replication of DNA Satellites and Their Role in Viral Pathogenesis

Cited by 2 publications

References 100 publications

Natural occurrence of mesta yellow vein mosaic virus and DNA-satellites in ornamental sunflower (Helianthus spp.) in Pakistan

Natural occurrence of mesta yellow vein mosaic virus and DNA-satellites in ornamental sunflower (Helianthus spp.) in Pakistan

Identifying Genetic Diversity of Cotton Leaf Curl Virus in Commercial Cotton (Gossypium hirsutum L.)

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