Replication of Clinical Measles Virus Strains in Hispid Cotton Rats

Wyde, Philip R.; Moore-Poveda, Donna K.; Daley, Natalie J.; Oshitani, Hitoshi

doi:10.1046/j.1525-1373.1999.d01-54.x

Cited by 22 publications

(6 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The previous data indicated that the measles virus also uses two receptors in cotton rats that are presumably homologous to the human molecules [15]. Previous studies have documented that cotton rats are susceptible to infection by both vaccine and wild-type measles virus without adaptation [12,13]. However, in regards to in vitro infection of CRL cells, the results of this study revealed that wtMeV does not grow efficiently under primary infection, obtaining the ability to adapt after one or more passages.…”

Section: Resultsmentioning

confidence: 99%

“…Cotton rats (Sigmodon hispidus) are unique rodents because of their susceptibility to human pathogens [9][10][11]. Since 1992, the cotton rat has been used in MeV research and previous studies have documented its susceptibility to infection with both vaccine and wtMeV [12,13]. The susceptibility of cotton rat to MeV infection via intranasal inoculation is of value to the investigation of measles pathogenesis and vaccination.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Adaptation of wild-type measles virus to cotton rat lung cells: E89K mutation in matrix protein contributes to its fitness

et al. 2009

View full text Add to dashboard Cite

Wild-type measles virus (wtMeV) adapted well to cotton rat lung (CRL) cells after serial passages. In order to evaluate the contributions of the individual genes of wtMeV for adaptation, whole genome sequences of the adapted and original viruses were determined and analyzed. The results showed that there were two mutations in the whole genome of the adapted virus. One mutation was located at the 265th nucleotide in the open reading frame (ORF) of the M gene, resulting in the substitution of the 89th amino acid from E (glutamate) to K (lysine). The other was a silent mutation located at the 4182nd nucleotide in the ORF of the L gene. It was demonstrated that the E89K mutation in the M protein is responsible for the adaptation of wtMeV MV99Y in CRL cells. Cotton rats were infected with adapted virus and the original strain via intranasal inoculation. Virus titer results showed that adapted strain replicated better than the original strain in cotton rat lungs. It is suggested that the E89K mutation also contributes to the enhancement of wtMeV replication in a cotton rat model infected intranasally. The results revealed that the E89K mutation in the M protein plays a key role in wtMeV adaptation in cotton rat and CRL cells.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Adaptation of wild-type measles virus to cotton rat lung cells: E89K mutation in matrix protein contributes to its fitness

et al. 2009

View full text Add to dashboard Cite

show abstract

“…Although many species of cotton rats have been classified in the wild, only two have been inbred and are currently used for biomedical research, S. hispidus and S. fulviventer . Over the years, S. hispidus has been shown to support replication of a broad spectrum of human respiratory viruses including respiratory syncytial virus (RSV) [1], measles virus [2,3], several adenovirus serotypes [4,5], para-influenza virus type 3 [6], and human metapneumo virus [7-9]. Fewer studies have been performed using S. fulviventer , although there is some suggestion that they are differences in permissiveness and pathogenesis to certain pathogens when compared to S. hispidus [6,10].…”

Section: Introductionmentioning

confidence: 99%

Modeling Human Respiratory Viral Infections in the Cotton Rat (Sigmodon hispidus)

Blanco

Boukhvalova

Pérez

et al. 2014

J Antivir Antiretrovir

View full text Add to dashboard Cite

For over three decades, cotton rats have been a preferred model for human Respiratory Syncytial Virus (RSV) infection and pathogenesis, and a reliable model for an impressive list of human respiratory pathogens including adenoviruses, para influenza virus, measles, and human metapneumo virus. The most significant contribution of the cotton rat to biomedical research has been the development of anti-RSV antibodies for prophylactic use in high-risk infants. More recently, however, the cotton rat model has been further explored as a model for infection with other respiratory viral pathogens including influenza and rhinovirus.Together with RSV, these viruses inflict the greatest impact on the human respiratory health.This review will focus on the characteristics of these new models and their potential contribution to the development of new therapies.

show abstract

“…The cotton rat ( Sigmodon hispidus ) has proven to be a semipermissive (titer of inoculum correlates with titer of output virus) small animal model for the study of MV pathogenesis. MV replicates in lung tissue, mediastinal lymph nodes and spleen and viral RNA can be found in peripheral blood lymphocytes [15] , [16] , [17] , [18] . In addition, there are differences in viral spread and the immune suppressive capacity between wild type and vaccine strains.…”

Section: Introductionmentioning

confidence: 99%

Cotton Rat (Sigmodon hispidus) Signaling Lymphocyte Activation Molecule (CD150) Is an Entry Receptor for Measles Virus

et al. 2014

View full text Add to dashboard Cite

Cotton rats (Sigmodon hispidus) replicate measles virus (MV) after intranasal infection in the respiratory tract and lymphoid tissue. We have cloned the cotton rat signaling lymphocytic activation molecule (CD150, SLAM) in order to investigate its role as a potential receptor for MV. Cotton rat CD150 displays 58% and 78% amino acid homology with human and mouse CD150, respectively. By staining with a newly generated cotton rat CD150 specific monoclonal antibody expression of CD150 was confirmed in cotton rat lymphoid cells and in tissues with a pattern of expression similar to mouse and humans. Previously, binding of MV hemagglutinin has been shown to be dependent on amino acids 60, 61 and 63 in the V region of CD150. The human molecule contains isoleucine, histidine and valine at these positions and binds to MV-H whereas the mouse molecule contains valine, arginine and leucine and does not function as a receptor for MV. In the cotton rat molecule, amino acids 61 and 63 are identical with the mouse molecule and amino acid 60 with the human molecule. After transfection with cotton rat CD150 HEK 293 T cells became susceptible to infection with single cycle VSV pseudotype virus expressing wild type MV glycoproteins and with a MV wildtype virus. After infection, cells expressing cotton rat CD150 replicated virus to lower levels than cells expressing the human molecule and formed smaller plaques. These data might explain why the cotton rat is a semipermissive model for measles virus infection.

show abstract

Replication of Clinical Measles Virus Strains in Hispid Cotton Rats

Cited by 22 publications

References 34 publications

Adaptation of wild-type measles virus to cotton rat lung cells: E89K mutation in matrix protein contributes to its fitness

Adaptation of wild-type measles virus to cotton rat lung cells: E89K mutation in matrix protein contributes to its fitness

Modeling Human Respiratory Viral Infections in the Cotton Rat (Sigmodon hispidus)

Cotton Rat (Sigmodon hispidus) Signaling Lymphocyte Activation Molecule (CD150) Is an Entry Receptor for Measles Virus

Contact Info

Product

Resources

About