Replication initiator proteins of Acinetobacter baumannii plasmids: An update note

Castro-Jaimes, Semiramis; Guerrero, Gabriela; Bello-López, Elena; Cevallos, Miguel A.

doi:10.1016/j.plasmid.2021.102616

Cited by 8 publications

(6 citation statements)

References 25 publications

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“…plasmids carried replicase genes and all of them belonged to a Rep_3 superfamily (pfam01051). Therefore, plasmid classification was conducted based on nucleotide sequence alignment with already classified replicase genes ( Castro-Jaimes et al, 2022 ; Li et al, 2022 ). Among the tet (X)-positive Rep_3 superfamily plasmids, a total of six homology groups were successfully identified, such as the dominant GR31 (82.1%, 23/28), GR26 (3.6%, 1/28), GR41 (3.6%, 1/28), and GR59 (3.6%, 1/28; Figure 2 ).…”

Section: Resultsmentioning

confidence: 99%

“…A maximum-likelihood phylogenetic tree of replication initiator proteins was constructed by MEGA-X version 10.1.8 and visualized by Evolview version 3.0 ( Kumar et al, 2018 ; Subramanian et al, 2019 ). Plasmid classification was performed according to the A. baumannii replicon typing scheme, with at least 80% nucleotide coverage and at least 75% nucleotide identity of replicase genes in the same group ( Bertini et al, 2010 ; Castro-Jaimes et al, 2022 ; Li et al, 2022 ). Similarly, the bacterial host ranges of different plasmid groups were also evaluated by querying the NCBI database with representative replicase genes (accessed 24 May 2022).…”

Section: Methodsmentioning

confidence: 99%

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Classification and molecular characteristics of tet(X)-carrying plasmids in Acinetobacter species

et al. 2022

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The rapid dissemination of plasmid-mediated tet(X) genes in Acinetobacter species has compromised the clinical effectiveness of tigecycline, one of the last-resort antibiotics. However, the classification strategy and homology group of tet(X)-positive Acinetobacter spp. plasmids remain largely unknown. In this study, we classified them by genome-based replicon typing, followed by analyses of structural characteristics, transferability and in vivo effect. A total of 34 plasmids distributed in at least nine Acinetobacter species were collected, including three tet(X3)-positive plasmids and one tet(X6)-positive plasmid from our genome sequencing results. Among them, there were 28 plasmids carrying Rep_3 superfamily replicase genes and classified into six homology groups, consisting of GR31 (82.1%), GR26 (3.6%), GR41 (3.6%), GR59 (3.6%), and novel groups GR60 (3.6%) and GR61 (3.6%). Our tet(X3)-positive plasmids pYH16040-1, pYH16056-1, and pYH12068-1 belonged to the dominant GR31 group, whereas the tet(X6)-positive plasmid pYH12068-2 was unclassified. Structurally, all tet(X)-positive GR31 plasmids shared similar plasmid replication (repB), stability (parA and parB) and accessory modules [tet(X) and sul2], and 97.6% of plasmid-mediated tet(X) genes in Acinetobacter species were adjacent to ISCR2. Conjugation and susceptibility testing revealed pYH16040-1, pYH16056-1, and pYH12068-2, carrying plasmid transfer modules, were able to mediate the mobilization of multiple antibiotic resistance. Under the treatment of tigecycline, the mortality rate of Galleria mellonella infected by pYH16040-1-mediated tet(X3)-positive Acinetobacter spp. isolate significantly increased when compared with its plasmid-cured strain (p < 0.0001). The spread of such plasmids is of great clinical concern, more effects are needed and will facilitate the future analysis of tet(X)-positive Acinetobacter spp. plasmids.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Classification and molecular characteristics of tet(X)-carrying plasmids in Acinetobacter species

et al. 2022

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“…Plasmids and genetic elements carrying ARGs were analyzed by blastn and Easyfig 2.2.5_win for the 10 isolates sequenced by Oxford Nanopore technology and 8 isolates from NCBI repository having full-assembled genomes (Supplementary Table S2 ) 41 , 42 . Plasmids transferability was tested in vitro 43 using rifampicin resistant A. baumannii strain BM4547 as recipient 44 .…”

Section: Methodsmentioning

confidence: 99%

Multiple host colonization and differential expansion of multidrug-resistant ST25-Acinetobacter baumannii clades

Lupo,

Valot,

Saras

et al. 2023

Sci Rep

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The Acinetobacter baumannii clonal lineage ST25 has been identified in humans and animals and found associated with outbreaks globally. To highlight possible similarities among ST25 A. baumannii of animal and human origins and to gather clues on the dissemination and evolution of the ST25 lineage, we conducted a phylogenetic analysis on n = 106 human and n = 35 animal A. baumannii ST25 genomes, including 44 sequenced for this study. Resistance genes and their genetic background were analyzed, as well. ST25 genomes are clustered into four clades: two are widespread in South America, while the other two are largely distributed in Europe, Asia and America. One particular clade was found to include the most recent strains and the highest number of acquired antibiotic resistance genes. OXA-23-type carbapenemase was the most common. Other resistance genes such as blaNDM-1, blaPER-7, and armA were found embedded in complex chromosomal regions present in human isolates. Genomic similarity among multidrug resistant ST25 isolates of either animal or human origin was revealed, suggesting cross-contaminations between the two sectors. Tracking the clonal complex ST25 between humans and animals should provide new insights into the mode of dissemination of these bacteria, and should help defining strategies for preserving global health.

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“…GR1 to GR19 are classified based on polymerase chain reaction (PCR) typing for the gene encoding RIPs (Bertini et al., 2010 ). Additionally, GR20 to GR61 have been proposed according to the above typing scheme, with at least 80% nucleotide coverage and at least 75% nucleotide identity of RIP genes in the same group (Castro‐Jaimes et al., 2022 ; Chen et al., 2022 ; Li et al., 2022 ). Notably, the genetic content encompassing RIPs and their adjacent DNA sequences within plasmids of Acinetobacter are similar, implying that some of the plasmids are within the same incompatibility group (Salgado‐Camargo et al., 2020 ).…”

Section: General Features Of Representative Mgesmentioning

confidence: 99%

“…Total DNA is directly extracted from the target environment and used as a template for PCR, allowing the detection of the presence or absence of plasmids based on amplification. Several primer sequences enabling multiplex PCR have been used to detect plasmids that are particularly important in the spread of ARGs (Carattoli, 2009 ; Carattoli et al., 2005 , 2014 ; Carloni et al., 2017 ; Castro‐Jaimes et al., 2022 ; Chen et al., 2022 ; Clewell, 2007 ; Li et al., 2022 ; Lozano et al., 2012 ). PCR‐Southern blot and quantitative PCR analyses using primers or probes specific to plasmid sequences have been used to detect the abundance of specific plasmids and their temporal changes in various environmental samples (Blau et al., 2020 ).…”

Section: Behaviours Of Mges In Natural Environmentsmentioning

confidence: 99%

Microbial evolution through horizontal gene transfer by mobile genetic elements

Tokuda,

Shintani

2024

Microbial Biotechnology

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Mobile genetic elements (MGEs) are crucial for horizontal gene transfer (HGT) in bacteria and facilitate their rapid evolution and adaptation. MGEs include plasmids, integrative and conjugative elements, transposons, insertion sequences and bacteriophages. Notably, the spread of antimicrobial resistance genes (ARGs), which poses a serious threat to public health, is primarily attributable to HGT through MGEs. This mini‐review aims to provide an overview of the mechanisms by which MGEs mediate HGT in microbes. Specifically, the behaviour of conjugative plasmids in different environments and conditions was discussed, and recent methodologies for tracing the dynamics of MGEs were summarised. A comprehensive understanding of the mechanisms underlying HGT and the role of MGEs in bacterial evolution and adaptation is important to develop strategies to combat the spread of ARGs.

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Replication initiator proteins of Acinetobacter baumannii plasmids: An update note

Cited by 8 publications

References 25 publications

Classification and molecular characteristics of tet(X)-carrying plasmids in Acinetobacter species

Classification and molecular characteristics of tet(X)-carrying plasmids in Acinetobacter species

Multiple host colonization and differential expansion of multidrug-resistant ST25-Acinetobacter baumannii clades

Microbial evolution through horizontal gene transfer by mobile genetic elements

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