Replication functions of pC194 are necessary for efficient plasmid transduction by M13 phage.

Dagert, M.; Jones, Ian M.; Goze, Albert; Romac, Stanka; Niaudet, B.; Ehrlich, S. Dusko

doi:10.1002/j.1460-2075.1984.tb01764.x

Cited by 47 publications

(34 citation statements)

References 37 publications

(19 reference statements)

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“…Of the three other plasmids from S. aureus that have been completely sequenced, both pC194 and pE194 contain open reading frames potentially coding polypeptides other than those responsible for antibiotic resistance Weisblum, 1982a, 1982b;Dagert et al, 1984). Furthermore, regions indispensable for replication have been identified on both plasmids and show striking similarities in their structural elements: a short G-C rich inverted complementary repeat (12-14 bp) adjacent to a longer inverted complementary repeat sequence (106-124 bp).…”

Section: Resultsmentioning

confidence: 99%

“…A high-copy number mutant, cop-6, of pE194 was attributed to a single base change (G to A) which mapped -100 bp from the shorter inverted complementary repeat. Although studies by Goze and Ehrlich (1980), and by Horinouchi and Weisblum (1982a) suggested that pE194-encoded polypeptides were unlikely to be required for replication, there is ample evidence to implicate a plasmid-specified protein in the replication of pC194 (Iordanescu and Surdeanu, 1983;Dagert et al, 1984).…”

Section: Resultsmentioning

confidence: 99%

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The use of synthetic oligonucleotides with universal templates for rapid DNA sequencing: results with staphylococcal replicon pC221.

Brenner¹,

Shaw²

1985

The EMBO Journal

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A rapid sequencing strategy has been devised and applied to determine the complete nucleotide sequence (4555 bp) of Staphylococcus aureus plasmid pC221. The entire replicon was cloned into phage M13mp8 in both orientations to provide 'universal templates' for primed DNA synthesis from internally-sited oligonucleotide primers. The latter were synthesized by a modification of a recently described paper disc method which employs phosphotriester chemistry. Less than 4 weeks was required for the synthesis of the required primers and for the sequencing experiments. Plasmid pC221 bears a substrate-inducible chloramphenicol acetyltransferase (CAT) gene that shares much homology with its counterparts in pC194 (S. aureus) and the chromosomal cat-86 gene of Bacillus pumilus, both in coding regions and upstream sequences believed to be involved in the induction phenomenon. A second plasmid-specified protein, REP D, has an 81% identity in the REP C polypeptide that has been shown to be essential for the replication of staphylococcal plasmid pT181. The 5' flanking region of rep D shows striking similarities with its counterpart in rep C that determines copy number and incompatibility. The nucleotide sequence reveals two additional and overlapping open reading frames that may specify proteins that play roles in plasmid relaxation and transfer.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

The use of synthetic oligonucleotides with universal templates for rapid DNA sequencing: results with staphylococcal replicon pC221.

Brenner¹,

Shaw²

1985

The EMBO Journal

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“…Standard transformation techniques were used in all cases. Plasmids pUC19 (41), pC194 (6,19), and pUB110 (25) have all been sequenced; the published nucleotide numbering has been maintained in this work (pC194 as described in reference 6). Plasmid pHV1160 was derived from pHV653 (26), by inserting a polylinker present in pGC2 (28) Fig.…”

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confidence: 99%

Replication origins of single-stranded-DNA plasmid pUB110

et al. 1989

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The two replication origins of plasmid pUB110 have been characterized. The site of initiation of DNA replication at the plus origin was mapped to within an 8-base-pair sequence. DNA synthesis initiated at the origin was made to terminate precociously in an inserted sequence of 18 base pairs that is homologous to a sequence in the origin. This suggests that pUB110 replicates as a rolling circle. The minus origin of plasmid pUB10 has been characterized, and the minimal sequence required for function has been determined. As with other minus origins, activity is orientation specific with respect to the direction of replication. Its activity is sensitive to rifampin in vivo, suggesting that RNA polymnerase catalyzes single-strand to double-strand conversion. Unlike all other plasmids of gram-positive bacteria thus far described, the pUB110 minus origin is functional in more than one host.

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“…Although in other experiments [12] insertion ofpBR322 DNAin the same HpuII site of pC 194 did not entail a loss of autonomous replication, both the transforming activity and the copy number of this hybrid plasmid were decreased. These experiments can hardly be compared to those reported in the present work.…”

Section: Discussionmentioning

confidence: 65%

Cloning of DNA segments of phage 2C, which allows autonomous plasmid replication in Bacillus subtilis

Lannoy¹,

Hoet²,

Cocito³

1985

Eur J Biochem

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The chromosome of Bacillus suhtilis phage 2 C is a 100-MDa double-stranded DNA molecule, containing hydroxymethyluracil in place of thymine and carrying redundant ends each encoinpassing 10% of the genome. 2C DNA was cleaved with EcoRI and HindIII, and cloned in the shuttle plasmids pSC540 and pCP 11 5, both containing segments originating from B. suhtilis and Escherichia coli plasmids. These chimaerical plasmids, carrying the chloramphenicol resistance gene, were unable to replicate in B. subtilis; this ability was restored, however, after the insertion of viral DNA segments. Physical maps of the recombinant plasmids were made; a large deletion of the E. coli-derived segment of pSC540 was observed (which paralleled a loss of replication in this host), whereas addition of 2C DNA segments in pCPll5 was not accompanied by deletion (replication in E. coli was conserved in this case). Cloned viral segments mapped mostly, but not exclusively, within the redundant ends of 2C DNA. It is suggested that the thirteen recombinant clones carried the replication origin region of phage 2C DNA, and that these sequences originated within or close to the redundant extremities of the viral chromosome.Isolation of the replication origin of bacterial and viral chromosomes is a prerequisite for elucidating their unique property as binding sites for specific enzymes and inhibitors. Additional features of the replication origin are its dependence on both transcriptional and translational specific polymerization processes, and its connection with the cell membrane [I]. The replication origins from bacteria, phages and plasmids show few structural homologies [l].The replication origin can be identified through its ability to confer autonomous replication to a nucleotide sequence (carrying a suitable antibiotic resistance marker) to which it is joined. In Escherichia coli, a plasmid capable of autonomous replication was constructed in this way, and the sequence acting as replication origin was identified [2-41. A similar approach in Bacillus subtilis led to the isolation of the replication origin of the defective bacteriophage PBSX 151. The failure to isolate an autonomous replicating unit from chromosomal B. subtilis DNA is attributed to the presence of two promoters in a ribosomal RNA operon; such a structure prevented the replication of the plasmid [6]. Sequences carrying replication functions were isolated from B. subtilis cryptic plasmids by joining them to appropriate selective markers [7].In addition to the origin of replication, transcription and translation of specific plasmidic sequences have been shown to be required for replication and stability of plasmids. The regulation of colEI plasmid replication indeed involved specific RNA transcripts hybridizing with the RNA primer at the origin of replication [8]. Plasniid-coded proteins are

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Replication functions of pC194 are necessary for efficient plasmid transduction by M13 phage.

Cited by 47 publications

References 37 publications

The use of synthetic oligonucleotides with universal templates for rapid DNA sequencing: results with staphylococcal replicon pC221.

The use of synthetic oligonucleotides with universal templates for rapid DNA sequencing: results with staphylococcal replicon pC221.

Replication origins of single-stranded-DNA plasmid pUB110

Cloning of DNA segments of phage 2C, which allows autonomous plasmid replication in Bacillus subtilis

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