Replication banding studies in two cyprinid fishes

Hellmer, Alexander; Voiculescu, I.; Schempp, Werner

doi:10.1007/bf00352203

Cited by 27 publications

(21 citation statements)

References 43 publications

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“…According to our results, and as proposed by some authors (Giles et al 1988, Hellmer et al 1991, the BrdU replication banding technique is the best method for chromosome identification in fish, and could be used for chromosome mapping studies in zebrafish. …”

Section: Resultssupporting

confidence: 55%

Chromosome complement, C-banding, Ag-NOR and replication banding in the zebrafishDanio reio

1996

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The chromosome complement of Danio rerio was investigated by Giemsa staining and C-banding, Ag-NORs and replication banding. The diploid number of this species is 2n = 50 and the arm number (NF) = 100. Constitutive heterochromatin was located at the centromeric position of all chromosome pairs. Nucleolus organizer regions appeared in the terminal position of the long arms of chromosomes 1, 2 and 8. Replication banding pattern allowed the identification of each chromosome pair.

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Section: Resultssupporting

confidence: 55%

Chromosome complement, C-banding, Ag-NOR and replication banding in the zebrafishDanio reio

1996

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“…Replication banding patterns have been obtained for several fish species (Delany and Bloom, 1984;AlmeidaToledo et al, 1988;Giles et al, 1988;Gold et al, 1990;Hellmer et al, 1991;Sánchez et al, 1993;Bertollo et al, 1997) after brief treatment with BrdU. A replication banding pattern was obtained in chromosome preparations of A. scabripinnis paranae using this technique (Figures 1a and 2a).…”

Section: Resultsmentioning

confidence: 87%

R- and G-band patterns in Astyanax scabripinnis paranae (Pisces, Characiformes, Characidae)

1999

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The absence of longitudinal bands in fish chromosomes has been associated with technical problems in chromosome preparations or the absence of a structural compartmentalization in the fish genome. In the present study, a R-banding pattern was obtained using a replication banding technique by in vivo treatment with 5-bromodeoxyuridine (5-BrdU). G-banding patterns were obtained after trypsin treatment and also after chromosome cleavage by in situ treatment with the restriction endonuclease BamHI. A similar G-banding pattern was also obtained after cleavage with the endonuclease HinfI. Presence of a resolute R- and G-banding patterns shows that Astyanax scabripinnis paranae chromosomes could present an isochore-like structure similar to that found in other vertebrates.
A ausência de bandas longitudinais em cromossomos de peixes tem sido associada a problemas técnicos nas preparações cromossômicas ou à ausência de uma compartimentalização estrutural no genoma dos peixes. No presente estudo, um padrão de bandas R foi obtido usando a técnica de bandamento de replicação pelo tratamento in vivo com 5-BrdU. Padrões de bandamento G foram obtidos após tratamento dos cromossomos com tripsina e também após clivagem in situ com a endonuclease de restrição BamHI. Um padrão similar de bandamento G também foi obtido após clivagem dos cromossomos com a endonuclease HinfI. A presença de um resolutível padrão de bandamento R e G em Astyanax scabripinnis paranae mostra que seus cromossomos parecem apresentar estrutura "isochore" similar àquela encontrada em outros vertebrados

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“…The fact that not all the chromosome pairs of the karyotypes of these salmonid species can be distinguished may not be too surprising since these species are of polyploid origin (Hartley, 1987). Nevertheless, the consistency and reproducibility of these banding patterns reinforce that the BrdU replication banding technique is one of the most sensitive methods for chromosomal analysis in fishes (Hellmer, Voiculescu & Schempp, 1991;Amores et al, 1995).…”

Section: Discussionmentioning

confidence: 98%

“…This problem can be overcome by means of 5-bromodeoxyuridine (BrdU) treatment. Replication banding patterns can be induced in species that do not show euchromatic bands (Sumner, 1990), including fishes (Almeida Toledo et al, 1998;Giles, Thode & Alvarez, 1988;Hellmer, Voiculescu & Schempp, 1991;Amores, Bejar & Alvarez, 1995;Daga, Thode & Almores, 1996).…”

Section: Introductionmentioning

confidence: 99%

Chromosomal evolution in salmonids: a comparison of Atlantic salmon, brown trout, and rainbow trout R-band chromosomes

Amaro

Abuín²,

Sánchez

1996

Genetica

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Replication banding technique was applied to the chromosomes of Salmo salar, Salmo trutta, and Oncorhynchus mykiss. The in vitro technique has proved more advantageous than the in vivo technique due to a higher number of bands obtained. The comparison of these replication banding patterns has revealed that some chromosomes of Salmo trutta karyotype appeared associated with Salmo salar and Oncorhynchus mykiss karyotypes by single chromosomal rearrangements.

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Replication banding studies in two cyprinid fishes

Cited by 27 publications

References 43 publications

Chromosome complement, C-banding, Ag-NOR and replication banding in the zebrafishDanio reio

Chromosome complement, C-banding, Ag-NOR and replication banding in the zebrafishDanio reio

R- and G-band patterns in Astyanax scabripinnis paranae (Pisces, Characiformes, Characidae)

Chromosomal evolution in salmonids: a comparison of Atlantic salmon, brown trout, and rainbow trout R-band chromosomes

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