Replication Banding in two Mediterranean Moray eels: Chromosomal Characterization and Comparison

Salvadori, Susanna; Coluccia, Elisabetta; Cannas, Rita; Cau, Angelo; Deiana, A. M.

doi:10.1023/b:gene.0000003649.64247.5b

Cited by 18 publications

(18 citation statements)

References 26 publications

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“…, 1991;Maistro et al . , 1999], characterization of homeologous chromosome regions [Giles et al, 1988;Salvadori et al, 2003], heterochromatin replication dynamics, and origin and diversification of sex chromosomes [Bertollo et al, 1997;Molina and Galetti, 2007]. Here, the array of late replicating bands along the chromosomes corresponded to the C-positive heterochromatin distribution for both species.…”

Section: Discussionmentioning

confidence: 83%

Paracentric Inversions Differentiate the Conservative Karyotypes in Two Centropomus Species (Teleostei: Centropomidae)

Borges

Cioffi

Bertollo

et al. 2019

Cytogenet Genome Res

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Centropomus is the sole genus of the Centropomidae family (Teleostei), comprising 12 species widely distributed throughout the Western Atlantic and Eastern Pacific, with 6 of them occurring in the Western Atlantic in extensive sympatry. Their life history and phylogenetic relationships are well characterized; however, aspects of chromosomal evolution are still unknown. Here, cytogenetic analyses of 2 Centropomus species of great economic value (C. undecimalis and C. mexicanus) were performed using conventional (Giemsa, Ag-NOR, and fluorochrome staining, C- and replication banding) and molecular (chromosomal mapping of 18S and 5S rDNA, H2A-H2B and H3 hisDNA, and (TTAGGG)_n repeats) approaches. The karyotypes of both species were composed of 48 solely acrocentric chromosomes (2n = 48; FN = 48), but the single ribosomal site was located in varying positions in the long arms of the second largest chromosome pair. Replication bands were generally similar, although conspicuous differences were observed in some chromosome regions. In both species, the histone H3 genes were located on 3 apparently homeologous chromosome pairs, but the exact position of these clusters differed slightly. Interspecific hisDNA and rDNA site displacements can indicate the occurrence of multiple paracentric inversions during the evolutionary diversification of the Centropomus genomes. Although the karyotypes remained similar in both species, our data demonstrate an unsuspected microstructural reorganization between them, driven most likely by a series of paracentric inversions.

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Section: Discussionmentioning

confidence: 83%

Paracentric Inversions Differentiate the Conservative Karyotypes in Two Centropomus Species (Teleostei: Centropomidae)

Borges

Cioffi

Bertollo

et al. 2019

Cytogenet Genome Res

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“…This failure has been attributed to technical problems in chromosome preparations (Gold et al 1990) or differences in the structure and composition of DNA from fish chromosomes compared to other vertebrates (Medrano et al 1988;Oliveira et al 2002). In spite of what has been mentioned above, karyotype analyses with G and R bands have contributed to comparative analyses between species and chromosome rearrangement detection (Gold et al 1990;Almeida-Toledo et al 1988;Vênere and Galetti 1995;Bertollo et al 1997;Maistro et al 1999;Vasconcelos and Martins-Santos 2000;DanielSilva andAlmeida-Toledo 2001, 2005;Salvadori et al 2003;Boroń 2003).…”

Section: Introductionmentioning

confidence: 99%

Occurrence of two cytotypes in Bryconamericus aff. iheringii (Characidae): karyotype analysis by C- and G-banding and replication bands

et al. 2007

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Cytogenetic analyses of Bryconamericus aff. iheringii specimens from the upper Paraná River basin (State of Paraná, Brazil) are provided. They had 2n = 52 chromosomes and two cytotypes with variations in their karyotypic formulae: cytotype I with 12 metacentric, 18 submetacentric, 8 subtelocentric and 14 acrocentric chromosomes with a fundamental number (FN) of 90; cytotype II with 8 metacentric, 28 submetacentric, 6 subtelocentric and 10 acrocentric chromosomes with a fundamental number (FN) of 94. Differences in C- and G-band patterns between the cytotypes, distinguishing marker chromosomes for each karyotype, were reported. The R-band pattern by 5-bromodeoxyuridine incorporation was obtained in chromosomes of the cytotype II sample. In some metaphases, the second pair of submetacentric chromosomes is distinctive: its short arm is heterochromatic (positive C-band), corresponding to a late replication region. In the same cytotype, a G- and R-band size heteromorphism w as recorded in the long arm of pair 9 (submetacentric). These methodologies revealed an actual karyotypic differentiation in the B. aff. iheringii population analyzed. Morphometrical comparative analyses and a discussion of evolutionary aspects of chromosome diversification in species of this genus are provided as well.

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“…Although scarce, karyotypical descriptions using replication bands in fishes have proved to be very elucidative, facilitating the pairing and the observation of homeologies among closely related species (Giles et al 1988;Almeida-Toledo et al 1988;Salvadori et al 2003), supporting a functional and dynamic chromosomal approach. Studies about replication bands in fishes bearing sex chromosomes are unusual (Bertollo et al 1997), and have not been performed in Anostomidae representatives so far.…”

Section: Introductionmentioning

confidence: 99%

Early replication banding in Leporinus species (Osteichthyes, Characiformes) bearing differentiated sex chromosomes (ZW)

Molina

Galetti

2006

Genetica

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There are few examples of differentiated sex chromosomes in fishes. In the genus Leporinus, seven species present a highly differentiated ZW system, derived from heterochromatinization process. Cytogenetic analyses carried out in three of these fish species, Leporinus obtusidens, L. elongatus and L. reinhardti, through RBG-banding, showed late replication bands, coincident with heterochromatic regions in both Z and W chromosomes. A similar interstitial early replication segment was observed in the complex heterochromatic region along the Wq arms in the three species, which might correspond to a pseudoautosomal segment (SD, sex determining locus). Asynchrony related to the replication pattern among different Z chromosomes was not observed. When the identification of nuclear organizer regions by silver nitrate was performed over chromosomal preparations previously exposed to 5-bromo-2'-deoxyuridine (BrdU), remarkable positive signals at interstitial and telomeric position were observed on the q arms of W chromosomes in the species L. elongatus and L. reinhardti. The absence of 18S ribosomal RNA gene loci in this region, formerly demonstrated by FISH, indicates that this argentophilic behavior is putatively due to heterochromatin decondensation caused by BrdU incorporation, favoring such Ag+ reaction. Early and late replication bands were also observed in the heterochromatic portions of Z and W chromosomes, indicating that euchromatic and heterochromatic regions are interspersed. The present data suggest a significant level of heterochromatic complexity in the sex chromosomes of each species. On the other hand, the replication pattern shared by them supports a monophyletic origin.

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Replication Banding in two Mediterranean Moray eels: Chromosomal Characterization and Comparison

Cited by 18 publications

References 26 publications

Paracentric Inversions Differentiate the Conservative Karyotypes in Two <b><i>Centropomus</i></b> Species (Teleostei: Centropomidae)

Paracentric Inversions Differentiate the Conservative Karyotypes in Two <b><i>Centropomus</i></b> Species (Teleostei: Centropomidae)

Occurrence of two cytotypes in Bryconamericus aff. iheringii (Characidae): karyotype analysis by C- and G-banding and replication bands

Early replication banding in Leporinus species (Osteichthyes, Characiformes) bearing differentiated sex chromosomes (ZW)

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