Replication and infectivity of the single-embedded nuclear polyhedrosis virus, Baculovirus heliothis, in homologous cell lines

An entomopoxvirus homologue of the vaccinia virus D13L-encoded 'rifampicin resistance' protein

“…Identical methods were employed here; larvae of both H. armigera (Ha) and Chrysodeixis argentifera (Ca; Lep., Noctuidae), a species previously recorded as susceptible to infection (Teakle, 1990), were used for in vivo HaEPV propagation. Techniques associated with in vitro propagation of HaEPV in Helicoverpa zea cells [BCIRL-Hz-AM1 (Hz-AM1); McIntosh & Ignoffo, 1981] or their derivatives, and Spodopterafrugiperda cells (Sf9; ATCC CRL 1711) are described elsewhere (Fernon et al, 1995). Unless otherwise noted, in vitro infections used a mixed population of HaEPV originating from Ha-derived virus (Fernon et al, 1995), and propagated in Hz-AM1 cells.…”

Production of Insecticidal Baculoviruses in Insect Cell Cultures: Potential and Limitations

“…In general, these lines were established from tissues of the natural host of the baculovirus to replicate, because viral yields tend to be higher in infected cultures of these homologous cell lines than in the heterologous ones. For instance, the cell lines saUFL-AG-286 (Anticarsia gemmatalis) (Sieburth & Maruniak, 1988), BM5 (Bombyx mori) (Grace, 1967), BCIRL-HZ-AM1 (Heliothis zea) (McIntosh & Ignoffo, 1981) and IPLB-LdEIta (Lymantria dispar) (Lynn et al, 1988) have been used to produce specifically the viruses AgMNPV, BmNPV, HaSNPV and LdMNPV, respectively. However, these cell lines are not as well characterized as the most widely used lines, and their technological properties (adaptation to suspension, ability to grow in serum-free culture media) are less remarkable or yet unknown.…”

Importance of virus–medium interactions on the biological activity of wild-type Heliothine nucleopolyhedroviruses propagated via suspension insect cell cultures

“…Better polyhedra yields can be obtained by rigorous screening of available cell lines, whether they be heterologous or homologous, heterogeneous or cloned (Lynn and Shapiro, 1998;McIntosh and Ignoffo, 1981;Rice et al, 1989;Sohi et al, 1984). Furthermore, the cell line itself may be a source of virus instability in the form of transposon-mediated mutagenesis (Fraser et al, 1985).…”

“…HaSNPV-UQ and HzSNPV-CY were provided courtesy of the Queensland Department of Primary Industries (Long Pocket Laboratories, Brisbane, Australia), and the American Cyanamid Company (Princeton, NJ), respectively. The H. zea cell line used in this study is BCIRL-HZ-AM1, which was originally derived from pupal ovarian tissue (McIntosh and Ignoffo, 1981) and was adapted to suspension cultures in both SFM for at least 10 passages before utilisation in experiments. VPM is an in-house SFM with a proprietary low-cost formulation.…”