Replicate hybrid zones suggest a limited role of plumage in reproductive isolation among subspecies of the Variable Seedeater (Sporophila corvina)

Abstract

After establishing secondary contact, recently diverged populations may remain reproductively isolated or hybridize to a varying extent depending on factors such as hybrid fitness and the strength of assortative mating. Replicated contact zones between hybridizing taxa offer a unique opportunity to explore how different factors interact to shape patterns of hybridization. Here, we used genomic and phenotypic data from three independent contact zones between subspecies of the Variable Seedeater (Sporophila corvina), to examine how coloration and genetic divergence shape patterns of hybridization. We found that plumage coloration has limited introgression across contact zones, but the degree of plumage divergence does not explain overall patterns of introgression. Across two parallel contact zones between populations with divergent phenotypes (entirely black vs. pied plumage) populations hybridized extensively across one contact zone but not the other, suggesting that plumage divergence is not sufficient to maintain reproductive isolation. Where subspecies hybridized, hybrid zones were wide and formed by later-generation hybrids, suggesting that hybrids present similar or higher fitness than parental subspecies. Moreover, contemporary gene flow has played an important role in shaping patterns of genetic diversity between populations. Overall, our results demonstrate that divergence in plumage coloration is important in reducing gene flow but insufficient in maintaining reproductive isolation in this clade, and that other factors such as divergence in song and time since secondary contact may also play an important role in driving patterns of reduced hybridization and gene flow.

Replicate contact zones suggest a limited role of plumage in reproductive isolation among subspecies of the variable seedeater (Sporophila corvina)

“…SNPs and phenotypic data are available on the Dryad data repository (Ocampo et al, 2023). “Scorvina_SNPs_1.vcf” and “Scorvina_SNPs_2.vcf” contain the SNP data sets according to our two filtering criteria.…”

triangulaR:an R package for identifying AIMs and building triangle plots using SNP data from hybrid zones

“…thousands) SNPs from which AIMs can be identified, but practices for sampling parental populations and identifying AIMs vary widely (e.g. Del-Rio et al, 2022;Ocampo et al, 2023;Preckler-Quisquater et al, 2023). The sample size needed for each parental group is an important consideration because any method for identifying AIMs depends on identifying genomic sites that reliably show differentiation between the parental groups (Rosenberg et al, 2003).…”