Replicase-Mediated Resistance To Potato Virus Y in Transgenic Tobacco Plants

Audy, Patrice; Palukaitis, Peter; Sa, Slack; Zaitlin, Milton

doi:10.1094/mpmi-7-0015

Cited by 93 publications

(34 citation statements)

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“…Replicase protein mediated resistance against a virus in the transgenic plant was first shown against Tomato mosaic tobamo virus (TMV) in tobacco plants containing a putative rep gene encoding 54 kDa replicase proteins (Golemboski et al, 1990). Similar resistance had developed for several viruses viz., Pea early browning virus (PEBV) (MacFarlane and Davis, 1992), Potato virus Y (PVY) (Audy et al, 1994) and Cucumber mosaic virus (CMV) (Hellwald and Palukaitis, 1995). In plants carrying a transgene derived from the replicase genes of Cowpea mosaic virus (CowMV) (Sijen et al, 1995) and Pepper mild mottle tobamo virus (PMMV) (Tenllado et al, 1996), it is clear with the involvement of a RNA-mediated with homology dependent resistance mechanisms.…”

Section: Expression Of Viral Proteins 211 Replicase Mediated Resistmentioning

confidence: 99%

Banana bunchy top disease (BBTD) symptom expression in banana and strategies for transgenic resistance: A review

Elayabalan

Subramaniam

Selvarajan

2015

Emir. J. Food Agric

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Bananas are one among the world's leading food crops after rice, wheat and maize. Banana cultivation is affected by various diseases. Among them, in globally banana bunchy top disease (BBTD) caused by the banana bunchy top virus (BBTV) and Fusarium wilt caused by the Fusarium oxysporum f.sp. cubense are the most serious diseases. BBTV is an isometric virus with a circular single stranded DNA (ssDNA) genome consisting of at least six components, BBTV DNA-1 to 6. The virus is transmitted by the banana aphid (Pentalonia nigronervosa). In this review paper, we are discussing the global status of BBTD, symptom expression in both vegetative and reproductive stage, resistant strategy and recent BBTV resistant banana clones development.

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Section: Expression Of Viral Proteins 211 Replicase Mediated Resistmentioning

confidence: 99%

Banana bunchy top disease (BBTD) symptom expression in banana and strategies for transgenic resistance: A review

Elayabalan

Subramaniam

Selvarajan

2015

Emir. J. Food Agric

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Section: Introductionmentioning

confidence: 99%

“…In this situation, viral replication within inoculated transgenic leaves or protoplasts is markedly reduced (9, 10). Resistance is thought to be achieved either by the establishment of a dysfunctional replicase complex that impairs protein synthesis (11)(12)(13)(14) or by gene silencing (15).The situation of strain-specific resistance reported for transgenic tobacco plants expressing the truncated CMV 2a (CMV, cucumber mosaic virus) replicase protein (10, 16) may be complex. Chlorotic spots or lesions were occasionally observed on inoculated leaves of one line of transgenic plants that otherwise exhibited resistance to systemic disease and minimal cell-to-cell movement when challenged with high inoculum levels of CMV (10).…”

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confidence: 99%

Viral RNA trafficking is inhibited in replicase-mediated resistant transgenic tobacco plants.

Nguyen

Lucas

Ding

et al. 1996

Proc. Natl. Acad. Sci. U.S.A.

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Transgenic tobacco (Nicotiana tabacum cv. Turkish Samsun NN) plants expressing a truncated replicase gene sequence from RNA-2 of strain Fny of cucumber mosaic virus (CMV) are resistant to systemic CMV disease. This is due to suppression of virus replication and cell-to-cell movement in the inoculated leaves of these plants. In this study, microinjection protocols were used to directly examine cellto-cell trafficking of CMV viral RNA in these resistant plants.CMV RNA fluorescently labeled with the nucleotide-specific TOTO-1 iodide dye, when coinjected with unlabeled CMV 3a movement protein (MP), moved rapidly into the surrounding mesophyll cells in mature tobacco leaves of vector control and untransformed plants. Such trafficking required the presence of functional CMV 3a MP. In contrast, coinjection of CMV 3a MP and CMV TOTO-RNA failed to move in transgenic resistant plants expressing the CMV truncated replicase gene. Furthermore, coinjection of 9.4-kDa fluorescein-conjugated dextran (F-dextran) along with unlabeled CMV 3a MP resulted in cell-to-cell movement of the F-dextran in control plants, but not in the transgenic plants. Similar results were obtained with viral RNA when the 30-kDa MP of tobacco mosaic virus (TMV) was coinjected with TMV TOTO-RNA into replicase-resistant transgenic tobacco expressing the 54-kDa gene sequence of TMV. However, in these transgenic plants, the TMV-MP was still capable of mediating cell-to-cell movement of itself and the 9.4-kDa F-dextran. These results indicate that an inhibition of cell-to-cell viral RNA trafficking is correlated with replicase-mediated resistance. This raises the possibility that the RNA-2 product is potentially involved in the regulation of cell-to-cell movement of viral infectious material during CMV replication.To systemically infect its host, a plant virus must be competent to replicate, move cell-to-cell via plasmodesmata, and both enter and egress the long-distance transport system of the phloem (1). Hence, strategies to develop transgenic plants resistant to viral infection have focused on reducing the efficacy of replication and/or cell-to-cell movement. Such strategies have included expression of viral coat protein (2, 3), or dysfunctional movement protein (MP; refs. 4 and 5), which result in either a delay or an inhibition of the establishment of systemic infection. Additionally, complete resistance for a number of virus diseases has been achieved through the expression of different forms of viral replicase genes (6-8). In this situation, viral replication within inoculated transgenic leaves or protoplasts is markedly reduced (9, 10). Resistance is thought to be achieved either by the establishment of a dysfunctional replicase complex that impairs protein synthesis (11)(12)(13)(14) or by gene silencing (15).The situation of strain-specific resistance reported for transgenic tobacco plants expressing the truncated CMV 2a (CMV, cucumber mosaic virus) replicase protein (10, 16) may be complex. Chlorotic spots or lesions were occasionally obser...

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“…'Samsun' (NN) expressing sense and antisense of PVY-CI (Line 10-E, obtained from H. Barker, SCRI) or PVY-NIb (Line 10-7; Audy et al, 1994) were used to assess recombination between PVA and a PVY transgene. These lines both express only low levels of transgene (unpublished data).…”

Section: Transgenic Plantsmentioning

confidence: 99%

No recombination detected in artificial potyvirus mixed infections and between potyvirus derived transgenes and heterologous challenging potyviruses

Dietrich

Miller²,

McKenzie³

et al. 2007

Environ. Biosafety Res.

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Replicase-Mediated Resistance To Potato Virus Y in Transgenic Tobacco Plants

Cited by 93 publications

References 0 publications

Banana bunchy top disease (BBTD) symptom expression in banana and strategies for transgenic resistance: A review

Banana bunchy top disease (BBTD) symptom expression in banana and strategies for transgenic resistance: A review

Viral RNA trafficking is inhibited in replicase-mediated resistant transgenic tobacco plants.

No recombination detected in artificial potyvirus mixed infections and between potyvirus derived transgenes and heterologous challenging potyviruses

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