Replacement of the Meniscus with a Collagen Implant (CMI)

Linke, Ralf D.; Ulmer, M.; Imhoff, Andreas B.

doi:10.1007/s00068-007-2188-7

Cited by 27 publications

(50 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Clinical findings have been inconclusive with regard to chondroprotection. [25][26][27][28][29] A key choice in the development of a tissue-engineered implant is whether to engineer a cellularized construct. [29][30][31][32][33][34][35][36] Cellularized scaffolds may be particularly important when the cells are necessary for maintenance of the surrounding matrix or have an important function, for example, preservation of the antithrombogenicity of a vascular conduit via endothelization or simply to aid continual growth in young recipients.…”

Section: Discussionmentioning

confidence: 99%

Investigation of the Regenerative Capacity of an Acellular Porcine Medial Meniscus for Tissue Engineering Applications

Stapleton

Ingram

Fisher

et al. 2011

Tissue Engineering Part A

View full text Add to dashboard Cite

Previously, we have described the development of an acellular porcine meniscal scaffold. The aims of this study were to determine the immunocompatibility of the scaffold and capacity for cellular attachment and infiltration to gain insight into its potential for meniscal repair and replacement. Porcine menisci were decellularized by exposing the tissue to freeze-thaw cycles, incubation in hypotonic tris buffer, 0.1% (w/v) sodium dodecyl sulfate in hypotonic buffer plus protease inhibitors, nucleases, hypertonic buffer followed by disinfection using 0.1% (v/v) peracetic, and final washing in phosphate-buffered saline. In vivo immunocompatibility was assessed after implantation of the acellular meniscal scaffold subcutaneously into galactosyltransferase knockout mice for 3 months in comparison to fresh and acellular tissue treated with a-galactosidase (negative control). The cellular infiltrates in the explants were assessed by histology and characterized using monoclonal antibodies against: CD3, CD4, CD34, F4/80, and C3c. Static culture was used to assess the potential of acellular porcine meniscal scaffold to support the attachment and infiltration of primary human dermal fibroblasts and primary porcine meniscal cells in vitro. The explants were surrounded by capsules that were more pronounced for the fresh meniscal tissue compared to the acellular tissues. Cellular infiltrates compromised mononuclear phagocytes, CD34-positive cells, and nonlabeled fibroblastic cells. T-lymphocytes were sparse in all explanted tissue types and there was no evidence of C3c deposition. The analysis revealed an absence of a specific immune response to all of the implanted tissues. Acellular porcine meniscus was shown to be capable of supporting the attachment and infiltration of primary human fibroblasts and primary porcine meniscal cells. In conclusion, acellular porcine meniscal tissue exhibits excellent immunocompatibility and potential for cellular regeneration in the longer term.

show abstract

Section: Discussionmentioning

confidence: 99%

Investigation of the Regenerative Capacity of an Acellular Porcine Medial Meniscus for Tissue Engineering Applications

Stapleton

Ingram

Fisher

et al. 2011

Tissue Engineering Part A

View full text Add to dashboard Cite

show abstract

“…In vivo results have been promising with human meniscal cartilage-like tissue growing into the collagen scaffold. 1,[11][12][13][14][15] However, initial biomechanics of the CMI are known to be poor. 16 This has been attributed to the random orientation of the CMI collagen fibers, which prevents the applied load being converted into circumferentially directed forces and associated hoop tensile stresses which are usually restrained by the circumferential collagen fibers found in native menisci.…”

Section: Introduction T He Meniscus Is a Fibrocartilage Structure Foundmentioning

confidence: 99%

Development and Characterization of an Acellular Porcine Medial Meniscus for Use in Tissue Engineering

Stapleton

Ingram²,

Katta³

et al. 2008

Tissue Engineering Part A

152

149

View full text Add to dashboard Cite

The objectives of this study were to characterize fresh porcine menisci and develop a decellularization protocol with a view to the generation of a biocompatible and biomechanically functional scaffold for use in tissue engineering/regeneration of the meniscus. Menisci were decellularized by exposing the tissue to freeze-thaw cycles, incubation in hypotonic tris buffer, 0.1% (w/v) sodium dodecyl sulfate in hypotonic buffer plus protease inhibitors, nucleases, hypertonic buffer followed by disinfection using 0.1% (v/v) peracetic acid and final washing in phosphate-buffered saline. Histological, immunohistochemical, and biochemical analyses of the decellularized tissue confirmed the retention of the major structural proteins. There was, however, a 59.4% loss of glycosaminoglycans. The histoarchitecture was unchanged, and there was no evidence of the expression of the major xenogeneic epitope, galactose-alpha-1,3-galactose. Biocompatibility of the acellular scaffold was determined by using contact cytotoxicity and extract cytotoxicity tests. Decellularized tissue and extracts were not cytotoxic to cells. Biomechanical properties were determined by indentation and tensile tests, which confirmed the retention of biomechanical properties following decellularization. In conclusion, this study has generated data on the production of a biocompatible, biomechanically functional scaffold for use in meniscal repair.

show abstract

“…Those patients showed more bone marrow edema and fewer good clinical results, measured by the Lysholm score, than with isolated CMI at 1 year of follow-up. Linke et al (2006) performed a controlled study involving 60 patients with varus morphotype and medial meniscus loss. The control group underwent high tibial valgus osteotomy (HTVO) while the treatment group underwent HTVO combined with medial CMI.…”

Section: Results Of Associated Proceduresmentioning

confidence: 99%