Replacement of the coat protein gene of plum pox potyvirus with that of zucchini yellow mosaic potyvirus: characterization of the hybrid potyvirus

Tóbiás, István; Palkovics, László; Tzekova, Lilia; Balázs, Ervin

doi:10.1016/s0168-1702(01)00241-6

Cited by 17 publications

(3 citation statements)

References 36 publications

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“…Thus, the N-terminus is likely relevant to the virus systemic movement perhaps via compromising the host defense response. Interestingly, the replacement of the CP gene in PPV with that of Zuccini yellow mosaic potyvirus (ZYMV) had no effect on the host range, with 85% similarity between CPs of PPV and ZYMV (Tóbiás et al, 2001). Also, pathogenicity, stability and replication capacity of the hybrid PPV were nearly identical to the parental viruses.…”

Section: Cps and Virus Host Rangementioning

confidence: 99%

Multiple functions of capsid proteins in (+) stranded RNA viruses during plant–virus interactions

Weber

Bujarski

2015

Virus Research

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Section: Cps and Virus Host Rangementioning

confidence: 99%

Multiple functions of capsid proteins in (+) stranded RNA viruses during plant–virus interactions

Weber

Bujarski

2015

Virus Research

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“…Most of the studies with PPV have been carried out to characterize the nucleotide sequence, identify the pathogenicity determinants and characterize the hybrid potyviruses at the morphological level, studying their replication and mapping for PPV resistance (Martín and García 1991, Sáenz et al 2000, Tóbiás et al 2001, Hurtado et al 2002). However, no data concerning the response of antioxidant systems to PPV infection at the subcellular level have been reported for woody plants.…”

Section: Introductionmentioning

confidence: 99%

Oxidative stress induced by long‐term plum pox virus infection in peach (Prunus persica)

Hernández

Rubio

Olmos

et al. 2004

Physiologia Plantarum

104

132

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In this study, the effect of long‐term plum pox virus (PPV) infection on the response of certain antioxidant enzymes at the subcellular level was studied in peach plants (Prunus persica (L.) Batch) (cv. GF305), which are characterized by great susceptibility to the virus. In infected plants, a decrease in the efficiency of excitation energy capture by PSII (Fv′/Fm′) was observed, which was accompanied by a decrease in non‐photochemical quenching (NPQ). p‐Hydroxy‐mercury benzoic acid (pHMB)‐insensitive ascorbate peroxidase (APX) activity (class III peroxidase) was detected in both chloroplast and soluble fractions. In soluble fractions from inoculated peaches, a significant increase in pHMB‐sensitive APX activity and a significant decrease in superoxide dismutase (SOD) activity were observed. These changes were correlated with the observations in isolated chloroplasts, where an increase in both pHMB‐sensitive and pHMB‐insensitive APX activities was observed, whereas significant decreases in SOD, monodehydroascorbate reductase (MDHAR) and glutathione reductase (GR) activities were produced. According to these results, as a consequence of PPV infection, an oxidative stress, indicated by an increase in lipid peroxidation and protein oxidation, was produced in peach leaves, which was monitored by the diaminobenzidine (DAB) peroxidase‐coupled H2O2 probe. PPV infection produced an alteration in chloroplast ultrastructure, giving rise to dilated thylakoid membranes. PPV‐infected peach leaves showed a decreased amount of starch in chloroplasts from palisade parenchyma, as well as an increase in the number and size of plastoglobuli, in relation to control plants. The results suggest that long‐term PPV infection produces an oxidative stress, and that an antioxidative metabolism imbalance may be related to the progress of PPV infection and symptoms in peach plants.

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“…25d ). Capability of the potyviral CP to encapsidate heterologous viral RNA under certain conditions in vivo was reported before 51 , 52 . While recombinant CP shows limited specificity, it is expected that in plants, in order to prevent wasting viral resources, the interplay between the viral and/or host factors is dictating packaging of the viral ssRNA into stable virions 11 , 53 .…”

Section: Discussionmentioning

confidence: 72%

From structural polymorphism to structural metamorphosis of the coat protein of flexuous filamentous potato virus Y

Kavčič,

Kežar,

Koritnik

et al. 2024

Commun Chem

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The structural diversity and tunability of the capsid proteins (CPs) of various icosahedral and rod-shaped viruses have been well studied and exploited in the development of smart hybrid nanoparticles. However, the potential of CPs of the wide-spread flexuous filamentous plant viruses remains to be explored. Here, we show that we can control the shape, size, RNA encapsidation ability, symmetry, stability and surface functionalization of nanoparticles through structure-based design of CP from potato virus Y (PVY). We provide high-resolution insight into CP-based self-assemblies, ranging from large polymorphic or monomorphic filaments to smaller annular, cubic or spherical particles. Furthermore, we show that we can prevent CP self-assembly in bacteria by fusion with a cleavable protein, enabling controlled nanoparticle formation in vitro. Understanding the remarkable structural diversity of PVY CP not only provides possibilities for the production of biodegradable nanoparticles, but may also advance future studies of CP’s polymorphism in a biological context.

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Replacement of the coat protein gene of plum pox potyvirus with that of zucchini yellow mosaic potyvirus: characterization of the hybrid potyvirus

Cited by 17 publications

References 36 publications

Multiple functions of capsid proteins in (+) stranded RNA viruses during plant–virus interactions

Multiple functions of capsid proteins in (+) stranded RNA viruses during plant–virus interactions

Oxidative stress induced by long‐term plum pox virus infection in peach (Prunus persica)

From structural polymorphism to structural metamorphosis of the coat protein of flexuous filamentous potato virus Y

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