Replacement of fishmeal with plant protein in the diets of juvenile lumpfish (Cyclopterus lumpus, L. 1758): Effects on digestive enzymes and microscopic structure of the digestive tract

Willora, Florence Perera; Vatsos, Ioannis N.; Mallioris, Panagiotis; Bordignon, Francesco; Keizer, Sven; Martínez‐Llorens, Silvia; Sørensen, Mette; Hagen, Ørjan

doi:10.1016/j.aquaculture.2022.738601

Cited by 22 publications

(9 citation statements)

References 94 publications

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“…Indeed, the SeE-SP5 and SeE-SP10 diets significantly influenced IFh and IGCn, indicating that these diets enhanced intestinal health. The PCA revealed that the fish meal replacement with soy protein concentrate at a level of 50% did not affect the growth of the juvenile lumpfish ( Cyclopterus lumpus ) and up to 70% did not affect the IFH and IGCn [ 70 ]. However, in our study, the SeE-SP supplementation at rates of SeE-SP5 and SeE-SP10 was observed to positively connect to IFH and IGCn, while the control and SeE-SP20 negatively impacted these variables.…”

Section: Discussionmentioning

confidence: 99%

Selenium-Enriched Spirulina (SeE-SP) Enhance Antioxidant Response, Immunity, and Disease Resistance in Juvenile Asian Seabass, Lates calcarifer

et al. 2022

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The present study examined the efficacy of dietary selenium-enriched spirulina (SeE-SP) on growth performance, antioxidant response, liver and intestinal health, immunity and disease resistance of Asian seabass, Lates calcarifer. A total of 480 seabass juveniles with an initial weight of 9.22 ± 0.09 g/fish were randomly assigned to four dietary groups. The fish were fed a fishmeal protein replacement diets with SeE-SP at 5%, 10%, and 20%, namely SeE-SP5, SeE-SP10, and SeE-SP20, and a fishmeal-based diet as control for 8 weeks. The results indicated that seabass juveniles fed SeE-SP5 and SeE-SP10 diets grew at the same rate as the fish fed a fishmeal-based control diet after 8 weeks of feeding, while SeE-SP20 grew at a significantly lower rate than the control (p < 0.05). Although most of the measured biochemical parameters were not influenced by the Se-SP diets, serum antioxidant-enzyme glutathione peroxidase (GPx) and immunological indices, such as lysozyme activity and immunoglobulin-M, were found significantly higher in the SeE-SP5 and SeE-SP10 diets compared to control. In addition, the fish fed the SeE-SP5 diet showed significantly lower mortalities after the 14-day of bacterial challenge with V. harveyi. These outcomes indicated that up to 10% inclusion of SeE-SP in the diet of juvenile Asian seabass does not compromise growth, while SeE-SP5 enhanced disease resistance in juvenile seabass.

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Section: Discussionmentioning

confidence: 99%

Selenium-Enriched Spirulina (SeE-SP) Enhance Antioxidant Response, Immunity, and Disease Resistance in Juvenile Asian Seabass, Lates calcarifer

et al. 2022

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“…Plant-based ingredients are detrimental to the intestinal absorption of carnivorous fish, and may induce intestinal inflammation ( Liu et al, 2018 ; Willora et al, 2022 ; Zhang et al, 2023 ). In addition to the effect of ANFs, it is suspected that a variety of other factors combine to influence the maintenance of normal intestinal physiology.…”

Section: Discussionmentioning

confidence: 99%

Effects of fermented soybean meal substitution for fish meal on intestinal flora and intestinal health in pearl gentian grouper

Pang

Peng

Xie³

et al. 2023

Front. Physiol.

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This study explored the role of replacing fish meal protein with fermented soybean meal (FSBM) protein on the growth performance and intestinal morphology, immunity, and microbiota of the pearl gentian grouper (Epinephelus fuscoguttatus♀ × E. lanceolatus♂). Three isonitrogenous and isolipidic diets with increasing levels of FSBM (0%, 20% and 40%; referred to as FM, FSBM20 and FSBM40 diets, respectively) as a replacement for fish meal were selected for this study. The pearl gentian grouper were fed these diets for 10 weeks. The findings revealed that the growth of fish fed the FSBM diets (FSBM20 and FSBM40) were remarkably lower than the fish fed the FM diet. Pathological manifestations of intestinal inflammation, such as shortened intestinal mucosal folds and thickened lamina propria, were observed in the fish fed the FSBM diets. Moreover, the gene expression levels of IL1β, IL12, IL17, and TNFα were remarkably upregulated in fish fed the FSBM40 diet, in contrast to the gene expression levels of IL4, IL5, IL10, and TGFβ1, which were remarkably downregulated (p < 0.05). The FSBM diets significantly affected the stability of the fish gut microbiota. Photobacterium was the dominant phylum in all experimental groups, and the proportion of these bacteria gradually decreased with increasing FSBM substitution. The composition of intestinal flora at the genus level was not the same in the three experimental groups, with a richer composition of intestinal bacteria detected in the FSBM20 and FSBM40 groups (p < 0.05). The correlation between intestinal flora balance and immune gene expression revealed that only Photobacterium was negatively correlated with the above upregulated genes, while other bacteria were positively correlated with these pro-inflammatory factors (p < 0.05). Photobacterium was positively correlated with the above downregulated genes, while other bacteria were negatively correlated with these anti-inflammatory factors (p < 0.05). In conclusion, high levels of substitution of FSBM for fish meal causes intestinal inflammation in pearl gentian grouper. This is likely associated with changes to the intestinal flora. More attention should be paid to the negative role of dietary FSBM on intestinal flora.

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“…A healthy digestive tract is important for maintaining proper immunity and growth in fish, and the health of the digestive tract is regulated by dietary ingredients [ 42 , 43 ]. Oxidative damage is an unavoidable part of the physiological activity of aerobic organisms, due to the continuous production of oxygen radicals during their physiological metabolism [ 44 ].…”

Section: Discussionmentioning

confidence: 99%

High Dietary Histamine Induces Digestive Tract Oxidative Damage in Juvenile Striped Catfish (Pangasianodon hypophthalmus)

Liu

Huang

et al. 2022

Antioxidants

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A 56-day feeding trial investigated the effects of dietary histamine on the antioxidant capacity, gastric and intestinal barrier functions, and growth performance of striped catfish (Pangasianodon hypophthalmus). Seven isonitrogenous (34.0% crude protein) and isolipidic (10.5% crude lipid) diets were formulated with supplemental 0, 15, 30, 60, 120, 240, and 480 mg/kg of histamine, named H0, H15, H30, H60, H120, H240, and H480 group, respectively. Results showed that the weight gain rate, specific growth rate, relative intestinal length in the H240 and H480 groups, and the condition factors in the H480 group were significantly lower than those in the H0 group. Intestinal total antioxidant capacity, peroxidase, catalase, superoxide dismutase, glutathione peroxidase, and glutathione reductase activities in the H480 group were significantly lower than those in the H0 group, whereas intestinal malondialdehyde content exhibited the opposite trend. Intestinal complement 3, complement 4, immunoglobulin M, and Recombinant Mucin 2 in the H480 group were significantly lower than those in the H0 group, in contrast to intestinal lipopolysaccharide content. Intestinal IL-10 gene expression in the H480 group was significantly lower than that in the H0 group, whereas the TNF-α, IL-1, IL-6, and IL-8 gene expression exhibited opposite results. Scanning and transmission electron microscopic observation of the gastrointestinal tract revealed severe damage to the gastric mucosa and intestinal epithelium in the H480 group. The abundance of Treponema in the histamine groups was significantly higher than that in the H0 group. These results indicated that high dietary histamine decreases intestinal immunity and antioxidant capacity, inducing digestive tract oxidative damage and ultimately decreasing the growth of striped catfish.

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Replacement of fishmeal with plant protein in the diets of juvenile lumpfish (Cyclopterus lumpus, L. 1758): Effects on digestive enzymes and microscopic structure of the digestive tract

Cited by 22 publications

References 94 publications

Selenium-Enriched Spirulina (SeE-SP) Enhance Antioxidant Response, Immunity, and Disease Resistance in Juvenile Asian Seabass, Lates calcarifer

Selenium-Enriched Spirulina (SeE-SP) Enhance Antioxidant Response, Immunity, and Disease Resistance in Juvenile Asian Seabass, Lates calcarifer

Effects of fermented soybean meal substitution for fish meal on intestinal flora and intestinal health in pearl gentian grouper

High Dietary Histamine Induces Digestive Tract Oxidative Damage in Juvenile Striped Catfish (Pangasianodon hypophthalmus)

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