Replacement of DHEA in Aging Men and Women

Yen, S. S. C.; Morales, Arlene J.; Khorram, Omid

doi:10.1111/j.1749-6632.1995.tb17377.x

Cited by 217 publications

(99 citation statements)

References 35 publications

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“…The antiproliferative effects of DHEA in SV cells from human adipose tissue corresponds with previous data from our laboratory, demonstrating the antiproliferative effects of DHEA in pig and rat SV preadipocytes 19 and in the 3T3-L1 preadipocyte cell line. 18 Furthermore, several clinical trials have demonstrated that treatment with DHEA reduces body fat 12,13 and increases lean body mass and muscle strength 14,15 in mature adults. Moreover, low levels of serum DHEA(S) have been correlated with high levels of body fat in both men 24,25 and women.…”

Section: Discussionmentioning

confidence: 99%

“…Indeed, many studies have shown that administration of DHEA, the intracellular form of the steroid, to rodents reduces adipose tissue mass. 2 ± 11 Moreover, several clinical trials have demonstrated that DHEA treatment reduces body fat 12,13 and increases both lean body mass 13 and muscle strength 14,15 in mature adults. Furthermore, recent studies by our group indicate that DHEAS is more effective than DHEA in reducing adipose tissue mass and cellularity in rats.…”

Section: Introductionmentioning

confidence: 99%

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Dehydroepiandrosterone alters the growth of stromal vascular cells from human adipose tissue

et al. 1999

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OBJECTIVE: The purpose of this study was to determine if the antiobesity actions of dehydroepiandrosterone (DHEA) observed in vivo are due to an in¯uence on the proliferation and differentiation of primary cultures of stromal-vascular (SV) cells isolated from human adipose tissue. DESIGN: SV cells were isolated from subcutaneous adipose tissue obtained from a young adult female undergoing elective liposuction. For the proliferation assay (Experiment 1), cultures were fed proliferation media containing 0, 5, 25 or 100 mM DHEA for 3 d. At the end of this treatment period, cultures were either prepared for counting or for determining their metabolic activity using the Alamar Blue staining procedure. For the differentiation assays (Experiment 2), cultures were fed differentiation media containing 0, 25 or 50 mM DHEA for 20 d. At the end of this treatment period, cultures were either prepared for lipid staining using Oil Red O or for marker enzyme analysis (glycerol-3-phosphate dehydrogenase activity; GPDH). To determine if the stimulatory effects of DHEA on SV cell differentiation were dependent on the presence of thiazolidinediones (Experiment 3), cultures of differentiating SV cells were incubated in the presence and absence of BRL 49653 and either 0, 25 or 50 mM DHEA. RESULTS: In Experiment 1, cultures treated with 25 and 100 mM DHEA had fewer cells than cultures treated with either 0 or 5 mM DHEA. Alamar Blue staining decreased as the level of DHEA in the cultures increased. In Experiment 2, cultures treated with DHEA had more lipid and GPDH activity than control cultures. In Experiment 3, cultures treated with BRL 49653 had more triglyceride than cultures treated without BRL 49653. Likewise, cultures treated with DHEA had more triglyceride than their non-DHEA controls. Regardless of the BRL status, cultures supplemented with DHEA had more triglyceride than control cultures. CONCLUSION: These data suggest that in cultures of SV cells from human adipose tissue, DHEA supplementation attenuates proliferation and enhances differentiation. These data support the hypothesis that DHEA directly attenuates preadipocyte proliferation in humans as we previously demonstrated in primary cultures of pig and rat SV cells and in cultures of 3T3-L1 preadipocytes. In contrast, DHEA stimulated the differentiation of human preadipocytes, which is contrary to its actions in differentiating cultures of preadipocytes from animals.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Dehydroepiandrosterone alters the growth of stromal vascular cells from human adipose tissue

et al. 1999

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“…Because there are marked declines in DHEAS with age (Orentreich et al, 1984;Š ulcová et al, 1997), researchers suspect it may be a marker of the aging process and potential longevity (Lopez, 1984;Kalimi and Regelson, 1999;Roth et al, 2002;Rotter et al, 1985;Rudman et al, 1990;Thomas et al, 1994;Yen, 2001). Viewed by some as a potential fountain of youth, DHEA supplementation has been reputed to prevent or ameliorate a myriad of illnesses and to enhance wellbeing (Baulieu et al, 2000;Morales et al, 1994;Shealy, 1995;South, 2002;Wolkowitz et al, 1997;Yen et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

Dehydroepiandrosterone sulfate (DHEAS) and health: does the relationship differ by sex?

Glei

Goldman

Weinstein

et al. 2004

Experimental Gerontology

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This study uses data from a large, nationally representative sample of older Taiwanese (aged 54 and older in 2000) to investigate sex differences in the relationship between DHEAS and various health outcomes. Data collection included an individual interview, a physical examination, and samples of blood and (12-h) urine. Regression models of health outcomes on DHEAS are estimated in two steps: first, including only controls for age and sex as well as an interaction between DHEAS and sex; and second, adding covariates likely to be related to both DHEAS and health outcomes (e.g. smoking). Results reveal that higher levels of DHEAS are associated with fewer mobility limitations (especially for women), better cognitive function (among women but not men), and better self-rated health (significant only for men but of similar magnitude for women). These findings are in contrast to previous studies conducted in the US and Europe that generally find stronger associations for men than women. Also unlike previous studies, which often demonstrate a negative relationship between DHEAS and depressive symptoms at least for women, we find little evidence of such a relationship for either sex. q 2004 Elsevier Inc. All rights reserved.

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“…13±15 Furthermore, low plasma levels of DHEAS have been inversely related to mortality among men; 16 the same was not found for women. 17 Moreover, several clinical trials have demonstrated that DHEA treatment reduces body fat 18,19 and increases lean body mass 19 and muscle strength 20,21 in mature adults.…”

Section: Introductionmentioning

confidence: 99%

Effects of acute administration of dehydroepiandrosterone-sulfate on adipose tissue mass and cellularity in male rats

1997

Int J Obes

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Objective: To determine if short term (2 week) treatment of growing male rats with low levels of dehydroepiandrosterone-sulfate (DHEAS) can reduce adiposity and serum triglycerides. Design: Rats were administered either normal drinking water or drinking water supplemented with 10 (D10) or 100 (D100) mg/ml DHEAS for 14 d. Subjects: Twenty-one male Sprague-Dawley rats (initial body weight 280 g). Measurements: Adipocyte mass, size and number from three major fat depots (retroperitoneal, epididymal, inguinal); serum levels of triglycerides, insulin, IGF-1 and DHEAS; brown adipose tissue (BAT) mass, uncoupling protein content and enzyme activity; body weight gain, food and water consumption; carcass composition. Results: DHEAS treatment had no effect on weight gain, food consumption or water intake. In contrast, rats treated with both levels of DHEAS had lighter fat pads, fewer epididymal and retroperitoneal adipocytes, less carcass lipid, lower levels of serum triglycerides and greater BAT mass and UCP content than control rats. Moreover, rats administered 100 mg/ml DHEAS had smaller and fewer epididymal adipocytes and fewer inguinal adipocytes than the D10 and the control rats. Conclusion: Acute treatment of growing male rats with low levels (10 mg/ml drinking water or 0.7 mg/kg body wt/d) of DHEAS reduces carcass lipid, adipose tissue mass and cellularity as well as serum triglycerides without altering food intake and body weight gain or causing hepatomegaly.

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Replacement of DHEA in Aging Men and Women

Cited by 217 publications

References 35 publications

Dehydroepiandrosterone alters the growth of stromal vascular cells from human adipose tissue

Dehydroepiandrosterone alters the growth of stromal vascular cells from human adipose tissue

Dehydroepiandrosterone sulfate (DHEAS) and health: does the relationship differ by sex?

Effects of acute administration of dehydroepiandrosterone-sulfate on adipose tissue mass and cellularity in male rats

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