Repetitive transcranial magnetic stimulation promotes functional recovery and differentiation of human neural stem cells in rats after ischemic stroke

Peng, Jinghui; Sha, Rong; Li, Mingxing; Chen, Luting; Han, Xiaohua; Guo, Feng; Chen, Hong; Huang, Xiaolin

doi:10.1016/j.expneurol.2018.12.002

Cited by 33 publications

(16 citation statements)

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“…Cell transplantation was performed seven days after 2VO as described previously [ 25 ]. In brief, MGE neural progenitors were dissociated with Accutase and prepared at approximately 1 × 10 5 cells/ μ L in the medium.…”

Section: Methodsmentioning

confidence: 99%

Electroacupuncture Promotes the Survival of the Grafted Human MGE Neural Progenitors in Rats with Cerebral Ischemia by Promoting Angiogenesis and Inhibiting Inflammation

Chen

et al. 2021

Neural Plasticity

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Stem cells have the potential as a regenerative therapy for cerebral ischemia by improving functional outcomes. However, cell transplantation has some limitations, including a low rate of the grafted cell survival. There is still a major challenge of promoting the harmonious symbiosis between grafted cells and the host. Acupuncture can effectively improve the functional outcome after cerebral ischemia. The present study evaluated the therapeutic effects and explored the mechanism of combined medial ganglionic eminence (MGE) neural progenitors differentiated from human embryonic stem cells (hESCs) with electroacupuncture (EA) in a bilateral common carotid artery occlusion (2VO) rat model. The results showed that EA could promote the survival of the grafted MGE neural progenitors differentiated from hESCs and alleviate learning and memory impairment in rats with cerebral ischemia. This may have partially resulted from inhibited expression of TNF-α and IL-1β and increased vascular endothelial growth factor (VEGF) expression and blood vessel density in the hippocampus. Our findings indicated that EA could promote the survival of the grafted MGE neural progenitors and enhance transplantation therapy’s efficacy by promoting angiogenesis and inhibiting inflammation.

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Section: Methodsmentioning

confidence: 99%

Electroacupuncture Promotes the Survival of the Grafted Human MGE Neural Progenitors in Rats with Cerebral Ischemia by Promoting Angiogenesis and Inhibiting Inflammation

Chen

et al. 2021

Neural Plasticity

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“…The RMT was defined as the lowest intensity of the TMS able to elicit a motor-evoked potential with amplitudes ≥15 µv at least 5/10 times ( 36 ). The rTMS parameters were set based on a previous study according to their efficacy and feasibility ( 37 ).…”

Section: Methodsmentioning

confidence: 99%

“…P0013B; Beyotime Institute of Biotechnology). The bicinchoninic acid (BCA) protein assay kit (Beyotime Institute of Biotechnology) was employed to estimate the protein concentrations, as detailed in a previous study ( 37 ). A total of 40 µg protein per lane was separated using 10% SDS-PAGE gels and then transferred onto PVDF membranes.…”

Section: Methodsmentioning

confidence: 99%

Repetitive transcranial magnetic stimulation increases neurological function and endogenous neural stem cell migration via the SDF‑1α/CXCR4 axis after cerebral infarction in rats

et al. 2021

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Neural stem cell (NSC) migration is closely associated with brain development and is reportedly involved during recovery from ischaemic stroke. Chemokine signalling mediated by stromal cell-derived factor 1α (SDF-1α) and its receptor CXC chemokine receptor 4 (CXCR4) has been previously documented to guide the migration of NSCs. Although repetitive transcranial magnetic stimulation (rTMS) can increase neurological function in a rat stroke model, its effects on the migration of NSCs and associated underlying mechanism remain unclear. Therefore, the present study investigated the effects of rTMS on ischaemic stroke following middle cerebral artery occlusion (MCAO). All rats underwent rTMS treatment 24 h after MCAO. Neurological function, using modified Neurological Severity Scores and grip strength test and NSC migration, which were measured using immunofluorescence staining, were analysed at 7 and 14 days after MCAO, before the protein expression levels of the SDF-1α/CXCR4 axis was evaluated using western blot analysis. AMD3100, a CXCR4 inhibitor, was used to assess the effects of SDF-1α/CXCR4 signalling. In addition, neuronal survival was investigated using Nissl staining at 14 days after MCAO. It was revealed that rTMS increased the neurological recovery of rats with MCAO, facilitated the migration of NSC, augmented the expression levels of the SDF-1α/CXCR4 axis and decreased neuronal loss. Furthermore, the rTMS-induced positive responses were significantly abolished by AMD3100. Overall, these results indicated that rTMS conferred therapeutic neuroprotective properties, which can restore neurological function after ischaemic stroke, in a manner that may be associated with the activation of the SDF-1α/CXCR4 axis.

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“…The study did not perform neurological evaluations, making it difficult to determine whether cell transplantation itself was carried out effectively, but the study did suggest that the TMS stimulation condition, which is generally too strong in intensity, may have had an adverse effect on the transplanted cells. Lastly, Peng et al studied the therapeutic effects of combining high-frequency rTMS with the transplantation of 2.5 × 10 5 hNSCs into the striatum four days after infarction in MCAO model rats [92]. The rTMS was started the day after cell transplantation and was administered without anesthesia to the ipsilesional hemisphere at an intensity of 26% of the maximum output and a frequency of 10 Hz for a 3 s duration, 10 times, with an interval of 50 s between each administration (300 pulses/day).…”

Section: Therapeutic Effects Of Combining Cell Transplantation and Brmentioning

confidence: 99%

Regenerative Rehabilitation for Stroke Recovery by Inducing Synergistic Effects of Cell Therapy and Neurorehabilitation on Motor Function: A Narrative Review of Pre-Clinical Studies

Ito

Kubo

Liang

et al. 2020

IJMS

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Neurological diseases severely affect the quality of life of patients. Although existing treatments including rehabilitative therapy aim to facilitate the recovery of motor function, achieving complete recovery remains a challenge. In recent years, regenerative therapy has been considered as a potential candidate that could yield complete functional recovery. However, to achieve desirable results, integration of transplanted cells into neural networks and generation of appropriate microenvironments are essential. Furthermore, considering the nascent state of research in this area, we must understand certain aspects about regenerative therapy, including specific effects, nature of interaction when administered in combination with rehabilitative therapy (regenerative rehabilitation), and optimal conditions. Herein, we review the current status of research in the field of regenerative therapy, discuss the findings that could hold the key to resolving the challenges associated with regenerative rehabilitation, and outline the challenges to be addressed with future studies. The current state of research emphasizes the importance of determining the independent effect of regenerative and rehabilitative therapies before exploring their combined effects. Furthermore, the current review highlights the progression in the treatment perspective from a state of compensation of lost function to that of a possibility of complete functional recovery.

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Repetitive transcranial magnetic stimulation promotes functional recovery and differentiation of human neural stem cells in rats after ischemic stroke

Cited by 33 publications

References 50 publications

Electroacupuncture Promotes the Survival of the Grafted Human MGE Neural Progenitors in Rats with Cerebral Ischemia by Promoting Angiogenesis and Inhibiting Inflammation

Electroacupuncture Promotes the Survival of the Grafted Human MGE Neural Progenitors in Rats with Cerebral Ischemia by Promoting Angiogenesis and Inhibiting Inflammation

Repetitive transcranial magnetic stimulation increases neurological function and endogenous neural stem cell migration via the SDF‑1α/CXCR4 axis after cerebral infarction in rats

Regenerative Rehabilitation for Stroke Recovery by Inducing Synergistic Effects of Cell Therapy and Neurorehabilitation on Motor Function: A Narrative Review of Pre-Clinical Studies

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