Repetitive sequences originating from the centromere constitute large-scale heterochromatin in the telomere region in the siamang, a small ape

Koga, Akihiko; Hirai, Yohei; Hara, Toru; Hirai, Hirohisa

doi:10.1038/hdy.2012.28

Cited by 20 publications

(48 citation statements)

References 30 publications

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“…These tandem repeats could provide sites for the activation of new centromeres, in accordance with the scenario proposed by Villasante et al [2007] on centromeres originating from telomeres. This evidence may be further supported by the correlation recently observed between sequences associated with telomeres and alpha satellite DNA found at centromeres [Adegoke et al, 1993;Garrido-Ramos et al, 1998;Cellamare et al, 2009;Koga et al, 2012;Prakhongcheep et al, 2013;Schneider et al, 2013].…”

supporting

confidence: 69%

Distribution of Interstitial Telomeric Sequences in Primates and the Pygmy Tree Shrew (Scandentia)

Mazzoleni

Schillaci²,

Sineo³

et al. 2017

Cytogenet Genome Res

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It has been hypothesized that interstitial telomeric sequences (ITSs), i.e., repeated telomeric DNA sequences found at intrachromosomal sites in many vertebrates, could be correlated to chromosomal rearrangements and plasticity. To test this hypothesis, we hybridized a telomeric PNA probe through FISH on representative species of 2 primate infraorders, Strepsirrhini (Lemur catta, Otolemur garnettii, Nycticebus coucang) and Catarrhini (Erythrocebus patas, Cercopithecus petaurista, Chlorocebus aethiops, Colobus guereza), as well as on 1 species of the order Scandentia, Tupaia minor, used as an outgroup for primates in phylogenetic reconstructions. In almost all primate species analyzed, we found a telomeric pattern only. In Tupaia, the hybridization revealed many bright ITSs on at least 11 chromosome pairs, both biarmed and acrocentric. These ITS signals in Tupaia correspond to fusion points of ancestral human syntenic associations, but are also present in other chromosomes showing synteny to only a single human chromosome. This distribution pattern was compared to that of the heterochromatin regions detected through sequential C-banding performed after FISH. Our results in the analyzed species, compared with literature data on ITSs in primates, allowed us to discuss different mechanisms responsible for the origin and distribution of ITSs, supporting the correlation between rearrangements and ITSs.

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supporting

confidence: 69%

Distribution of Interstitial Telomeric Sequences in Primates and the Pygmy Tree Shrew (Scandentia)

Mazzoleni

Schillaci²,

Sineo³

et al. 2017

Cytogenet Genome Res

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“…The whole fosmid DNAs of clones 14 and 22 were labelled and hybridized with the owl monkey chromosomes. The methods and conditions were the same as those in our previous work, 16 including the labelling reagents for the green (OwlAlp1) and red (OwlAlp2) colours. The bar represents 10 μm.…”

Section: Resultsmentioning

confidence: 99%

“…16–18 Briefly, a genomic library was prepared, individual clones were amplified in 96-well plates, transferred to membranes, hybridized with labelled genomic DNA, and clones responsible for strong signals were selected. The vector for the library was the fosmid pCC1FOS, and insert DNAs were 40- to 44-kb genomic DNA fragments produced by mechanical shearing and subsequent recovery from an electrophoresis gel.…”

Section: Methodsmentioning

confidence: 99%

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Two Types of Alpha Satellite DNA in Distinct Chromosomal Locations in Azara's Owl Monkey

et al. 2013

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Alpha satellite DNA is a repetitive sequence known to be a major DNA component of centromeres in primates (order Primates). New World monkeys form one major taxon (parvorder Platyrrhini) of primates, and their alpha satellite DNA is known to comprise repeat units of around 340 bp. In one species (Azara's owl monkey Aotus azarae) of this taxon, we identified two types of alpha satellite DNA consisting of 185- and 344-bp repeat units that we designated as OwlAlp1 and OwlAlp2, respectively. OwlAlp2 exhibits similarity throughout its entire sequence to the alpha satellite DNA of other New World monkeys. The chromosomal locations of the two types of sequence are markedly distinct: OwlAlp1 was observed at the centromeric constrictions, whereas OwlAlp2 was found in the pericentric regions. From these results, we inferred that OwlAlp1 was derived from OwlAlp2 and rapidly replaced OwlAlp2 as the principal alpha satellite DNA on a short time scale at the speciation level. A less likely alternative explanation is also discussed.

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“…11 The vector was the 8.1-kb fosmid pCC1FOS and the insert was 40-44 kb of genomic DNA fragments that had been generated by mechanical shearing and isolated by gel electrophoresis and subsequent recovery from a gel piece. This library was screened by the modified CGH technique 8 for highly repetitive sequences.…”

Section: Experiments Involving Dna Manipulationsmentioning

confidence: 99%

Tandem repeat sequences evolutionarily related to SVA-type retrotransposons are expanded in the centromere region of the western hoolock gibbon, a small ape

et al. 2012

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Hoolock hoolock (the western hoolock gibbon) is a species of the family Hylobatidae (small apes), which constitutes the superfamily Hominoidea (hominoids) together with Hominidae (great apes and human). Here, we report that centromeres or their vicinities in this gibbon species contain tandem repeat sequences that consist of 35-50-bp repeat units, and exhibit a sequence similarity with the variable number of tandem repeat (VNTR) region of the SVA, LAVA and PVA transposons. SVA is a composite retrotransposon thought to have been formed by fusion of three solo elements in the common ancestor of hominoids. LAVA and PVA are recently identified retrotransposons that have the same basic structure as SVA. Thus, the large-scale tandem repeats in the centromere region may have been derived from one or more of SVA-type transposons, including the three mentioned above and other yet unknown elements, or the repeat sequences could have served as a source for such elements. Amplification of VNTR-related sequences in another gibbon species, Hoolock leuconedys (eastern hoolock gibbon), has recently been reported, but it is yet to be examined whether the large-scale tandem repeats observed in the two species originated from a single event that occurred in their common ancestor. The repeat sequences in the western hoolock gibbon are mostly 40 kb or more in length, are present in 28 of the 38 chromosomes of the somatic cells, and are homozygous for chromosomal presence/ absence.

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Repetitive sequences originating from the centromere constitute large-scale heterochromatin in the telomere region in the siamang, a small ape

Cited by 20 publications

References 30 publications

Distribution of Interstitial Telomeric Sequences in Primates and the Pygmy Tree Shrew (Scandentia)

Distribution of Interstitial Telomeric Sequences in Primates and the Pygmy Tree Shrew (Scandentia)

Two Types of Alpha Satellite DNA in Distinct Chromosomal Locations in Azara's Owl Monkey

Tandem repeat sequences evolutionarily related to SVA-type retrotransposons are expanded in the centromere region of the western hoolock gibbon, a small ape

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