Repetitive Sequences in Eukaryotic DNA and their Expression

Jelinek, Warren R.; Schmid, Carl W.

doi:10.1146/annurev.bi.51.070182.004121

Cited by 575 publications

(284 citation statements)

References 2 publications

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“…Thus, an oligonucleotide probe of 16 nucleotides would be expected to be matched with and to hybridize to a human genome only once. However, it is well known that many repeated sequences, such as moderately repetitive DNAs, highly repetitive DNAs, RNA genes (rRNA, tRNA), satellite DNA, histone gene and a large amount of poly A sequences, constitute over 50 ~ of human genome (Britten and Davidson, 1971 ;Darnell, 1976Darnell, , 1983Pardue and Gall, 1970;Singer and Skowronski, 1985;Sinclair and Brown, 1971;Jelinek and Schmid, 1982). For example, repeated sequences with four identical nucleotides (AAAA or GGGG or CCCC or TTTT) can be matched 9.1 x 105 times in the total DNA sequence (8.3 x 107 bases) entered in GenBank (see Release 71.0 of GenBank, Bilofsky et al, 1986).…”

Section: Resultsmentioning

confidence: 99%

A mathematically designed STS primer without any mismatches for direct sequencing of cosmid DNA clones

et al. 1993

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SummaryWe report here a new method for the direct sequencing of large DNA inserts of cosmid clones from human chromosomes using STS primers of 14 nucleotides without any mismatches, which are designed from results of a mathematical analysis. It is clear that STS primer of 14 nucleotides is optimum for direct sequencing of cosmid recombinant DNA clones. We also provide examples of direct sequencing of cosmid clones of human chromosome 21 using these STS primers.

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Section: Resultsmentioning

confidence: 99%

A mathematically designed STS primer without any mismatches for direct sequencing of cosmid DNA clones

et al. 1993

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“…By comparing the three sequences, we mapped the crossover in tpr-met within one of three A residues at position 525 to 527 of the met gene ( Fig. 2A and C (12).…”

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confidence: 99%

“…Other members of the Alii family are, on average, 90% homologous (12). Alu repeats are often followed by long stretches of A residues (12), and the tpr-met arrangement occurs at the end of the poly(A) stretch following the tpr Alui sequence.…”

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Characterization of the rearranged tpr-met oncogene breakpoint.

Dean¹,

Park²,

Woude³

1987

Mol. Cell. Biol.

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We determined the nucleotide sequence of the rearranged trp-met genomic locus and the corresponding portions of the unrearranged tpr and met genomic fragments. The breakpoints occur at one end of a stretch of 21 A residues that follow an Alu repetitive sequence in the tpr locus and within a group of 3 A residues in the met proto-oncogene locus. We concluded that the fusion between the tpr locus on chromosome 1 and the met locus on chromosome 7 resulted from a recombination event.

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“…El parásito contiene 14 cromosomas que tienen una estructura lineal convencional, caracterizada por presentar homologia entre sus repeticiones teloméricas (l,2); los cromosomas se encuentran funcionalmente comparlimentalizados en regiones conservadas de dominio central y regiones polimórficas de dominio terminal. Los genes constitutivos (housekeeping genes) están localizados en la región central conservada del Algunas de estas secuencias son transcritas en pequeñas moléculas de ARN que tienen modificaciones en los extremos 3' y 5' (15). Se han propuesto algunas posibles funciones para el ADN repetitivo, por ejemplo, que el incremento en el volumen nuclear pueda aumentar la duración del ciclo de vida de la célula y que los elementos repetitivos causen recombinación ilegítima, introduzcan senales de regulación para la transcripción o nuevos sitios para el ~rocesamiento o maduración del mARN (1 3.16).…”

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Ubicación de la secuencia repetitiva PFCOL692 en fragmentos genómicos de Plasmodíum falciparum

Alvarado¹,

Rojas²,

Guerrero³

et al. 2000

biomedica

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ResumenLas secuencias repetitivas son componentes estructurales de los genomas eucariotes. Se desconoce la función de la mayoría de ellas, pero, al parecer, no son simplemente ADN egoísta sino que intervienen en procesos de recombinación y regulación génica. En este estudio se estableció la localización subtelomérica d e l a secuencia repetitiva PFCOL692 en los cromosomas de Plasmodium falciparum, a través de la caracterización de cuatro clones de la genoteca hEMBL4lPFCOL692, que contenían insertos entre 15 y 23Kb de ADN genómico del parásito; esta caracterización se hizo mediante análisis de restricción y la posible ubicación de PFCOL692 en el genoma se exploró utilizando sondas específicas para las regiones telomérica (pTB4.1) y subtelomérica (pRep20) de los cromosomas de P falciparum. El análisis de los mapas de restricción obtenidos permitió plantear una posible ubicación de PFCOL692 en el extremo de los cromosomas del parásito, donde las copias de esta secuencia se encuentran agrupadas en un segmento del subtelómero y con una posición ~0 n s e~a d a con respecto a la secuencia pRep20 Se sugiere, además, que PFCOL692 no se encuentra en el limite entre el subtelómero y el telómero.Palabras clave: Plasmodium falciparum, secuencias repetitivas, región subtelomérica Location o f the repetitive sequence PFCOL692 in genomic fragments of Plasmodium falciparum AbstractIn this paper we established the subtelomeric localization of the repetitive sequence PFCOL692 of Plasmodium falciparum by the characterization of four clones from a hEMBL41 PFCOL692 genomic library, which contains 15-23Kbp long inserts of parasite genomic DNA. Characterization was made by restriction analysis and the PFCOL692 localization in the genome was explored using specific probes for telomeric (pTB4.1) and subtelomeric regions (pRep20) of P falciparum chromosomes. We proposed a possible organization for the parasite chromosomes ends, where the copies of PFCOL692 are clusters in the subtelomeric region and their localization is conserved with respect to the pRep20 sequence. In addition, we established that PFCOL692 is not located next to the telomere.

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Repetitive Sequences in Eukaryotic DNA and their Expression

Cited by 575 publications

References 2 publications

A mathematically designed STS primer without any mismatches for direct sequencing of cosmid DNA clones

A mathematically designed STS primer without any mismatches for direct sequencing of cosmid DNA clones

Characterization of the rearranged tpr-met oncogene breakpoint.

Ubicación de la secuencia repetitiva PFCOL692 en fragmentos genómicos de Plasmodíum falciparum

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