Repetitive element-polymerase chain reaction for genotyping of clinical and environmental isolates of Legionella spp.

Haroon, Attiya; Koide, Michio; Higa, Futoshi; Hibiya, Kenji; Tateyama, Masao; Fujita, Jiro

doi:10.1016/j.diagmicrobio.2010.04.001

Cited by 7 publications

(6 citation statements)

References 20 publications

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“…PFGE is one of the molecular techniques indicated for comparison of the genomic profiles among Legionella strains of different origin by the National Guidelines (Linee Guida per la Prevenzione e il Controllo della Legionellosi, 2015 ). However, our results suggest that the use of other molecular techniques could be a useful tool that more accurately characterizes the identity of circulating Legionella strains, according to the results found in other studies (Haroon et al, 2010 ; Yamamoto et al, 2003 ; Yan et al, 2008 ).…”

Section: Discussionsupporting

confidence: 86%

“…PFGE, which is known as a laborious and time-consuming technique (Dilger et al, 2016 ), was initially used. This was followed by RAPD, rep-PCR, and SAU-PCR analysis, which are rapid, simple, reproducible, and inexpensive (Haroon et al, 2010 ). Although these techniques are not usually applied to L-np (Haroon et al, 2010 ; Corich et al, 2005 ; Yamamoto et al, 2003 ), they demonstrated a greater discriminative power than PFGE, allowing us to establish that not all of the isolated strains belonged to the same clone.…”

Section: Discussionmentioning

confidence: 99%

“…However, the use of different molecular techniques (e.g., pulsed-field gel electrophoresis (PFGE), RAPD, Rep-PCR, and SAU-PCR) is required to assess whether clinical patient isolates correspond to environmental isolates (Linee Guida per la prevenzione e il Controllo della Legionellosi, 2015 ; Haroon et al, 2010 ; Corich et al, 2005 ; Yamamoto et al, 2003 ).…”

Section: Introductionmentioning

confidence: 99%

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Legionella anisa or Legionella bozemanii? Traditional and molecular techniques as support in the environmental surveillance of a hospital water network

Giglio

D’Ambrosio

Spagnuolo

et al. 2023

Environ Monit Assess

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Understanding the actual distribution of different Legionella species in water networks would help prevent outbreaks. Culture investigations followed by serological agglutination tests, with poly/monovalent antisera, still represent the gold standard for isolation and identification of Legionella strains. However, also MALDI-TOF and mip-gene sequencing are currently used. This study was conducted to genetically correlate strains of Legionella non pneumophila (L-np) isolated during environmental surveillance comparing different molecular techniques. Overall, 346 water samples were collected from the water system of four pavilions located in a hospital of the Apulia Region of Italy. Strains isolated from the samples were then identified by serological tests, MALDI-TOF, and mip-gene sequencing. Overall, 24.9% of water samples were positive for Legionella, among which the majority were Legionella pneumophila (Lpn) 1 (52.3%), followed by Lpn2-15 (20.9%), L-np (17.4%), Lpn1 + Lpn2-15 (7.1%), and L-np + Lpn1 (2.3%). Initially, L-np strains were identified as L. bozemanii by monovalent antiserum, while MALDI-TOF and mip-gene sequencing assigned them to L. anisa. More cold water than hot water samples were contaminated by L. anisa (p < 0.001). PFGE, RAPD, Rep-PCR, and SAU-PCR were performed to correlate L. anisa strains. Eleven out of 14 strains identified in all four pavilions showed 100% of similarity upon PFGE analysis. RAPD, Rep-PCR, and SAU-PCR showed greater discriminative power than PFGE.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Legionella anisa or Legionella bozemanii? Traditional and molecular techniques as support in the environmental surveillance of a hospital water network

Giglio

D’Ambrosio

Spagnuolo

et al. 2023

Environ Monit Assess

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“…Repetitive element-polymerase chain reaction (rep-PCR) [9] was used to compare clinical and environmental L. pneumophila isolates. The clinical strain and only the ones isolated from home shower showed identical restriction pattern (Figure 1).…”

Section: Environmental Investigationmentioning

confidence: 99%

A Culture-Proven Case of Community-AcquiredLegionellaPneumonia Apparently Classified as Nosocomial: Diagnostic and Public Health Implications

Bargellini

Marchesi²,

Marchegiano³

et al. 2013

Case Reports in Medicine

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We report a case of Legionella pneumonia in a 78-year-old patient affected by cerebellar haemangioblastoma continuously hospitalised for 24 days prior to the onset of overt symptoms. According to the established case definition, this woman should have been definitely classified as a nosocomial case (patient spending all of the ten days in hospital before onset of symptoms). Water samples from the oncology ward were negative, notably the patient's room and the oxygen bubbler, and the revision of the case history induced us to verify possible contamination in water samples collected at home. We found that the clinical strain had identical rep-PCR fingerprint of L. pneumophila serogroup 1 isolated at home. The description of this culture-proven case of Legionnaires' disease has major clinical, legal, and public health consequences as the complexity of hospitalised patients poses limitations to the rule-of-thumb surveillance definition of nosocomial pneumonia based on 2–10-day incubation period.

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“…Fifty-nine strains of different Legionella species were used in this study, which included 36 strains of L. pneumophila (1 type strain and 35 clinical strains) [6]. The clinical L. pneumophila strains were collected in our laboratory and included 27 strains of the L. pneumophila serogroup (SG) 1, two strains of SG2, two strains of SG3, three strains of SG5, and one strain of SG10 (Table 1).…”

mentioning

confidence: 99%

Identification of Legionella pneumophila serogroups and other Legionella species by mip gene sequencing

Haroon

Koide

Higa

et al. 2012

Journal of Infection and Chemotherapy

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Repetitive element-polymerase chain reaction for genotyping of clinical and environmental isolates of Legionella spp.

Cited by 7 publications

References 20 publications

Legionella anisa or Legionella bozemanii? Traditional and molecular techniques as support in the environmental surveillance of a hospital water network

Legionella anisa or Legionella bozemanii? Traditional and molecular techniques as support in the environmental surveillance of a hospital water network

A Culture-Proven Case of Community-AcquiredLegionellaPneumonia Apparently Classified as Nosocomial: Diagnostic and Public Health Implications

Identification of Legionella pneumophila serogroups and other Legionella species by mip gene sequencing

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